Strain Name: C57BL/6J-Irf8m1Btlr/Mmucd
Stock Number: 034294-UCD
Other Names:
- Gemini, C57BL/6J-Irf8m1Btlr/Mmcd
Gene Details: (provided by MGI)
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Allele Symbol: Irf8m1Btlr Name: mutation 1, Bruce Beutler Alteration at locus: Chemically Induced |
Genetic Alterations:
The Gemini
mutation corresponds to a C to T transition at position 387 of the Irf8
transcript in exon 3 of 9 total exons using Genbank record NM_008320.
This converts a glutamine at amino acid 118 to a stop codon removing 307
amino acids from the C-terminus of the encoded protein.
Genotype Determination:
ES Cell Line: Not applicable
Strain Description
Phenotype
Homozygous phenotype: In the spleen,
there is a reduction in the frequency of mature follicular B cells and an
expansion of the marginal zone B cell compartment. Complete absence of
monocytes and mature macrophages in the blood, peritoneal cavity, and
bone marrow. There is also expansion of granulocytes, most likely
neutrophils, in the blood, spleen, and bone marrow. The mice are unable
to make type I interferon (IFN) after CpG DNA challenge in vivo. Animals
are susceptible to non-lethal doses of MCMV.
Heterozygous phenotype: Heterozygous animals may have an intermediate phenotype but this has not
been confirmed.
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
Irf8m1Btlr/Irf8m1Btlr
C57BL/6J-Irf8<m1Btlr>- hematopoietic system phenotype
- immune system phenotype
- abnormal interferon secretion (MGI Ref ID J:165017)
- non-secretion of type I interferon by plasmacytoid dendritic cells (pDCs) in response to in vivo stimulation by the toll-like receptor ligand, CpG
- decreased macrophage cell number (MGI Ref ID J:165017)
- decreased numbers of mature macrophages
- decreased monocyte cell number (MGI Ref ID J:165017)
- increased neutrophil cell number (MGI Ref ID J:165017)
- abnormal interferon secretion (MGI Ref ID J:165017)
Founder genetic background: C57BL/6J
Strain genetic background: C57BL/6J
Strain Development: The Gemini strain was generated by ENU on a C57BL/6J background and was maintained on this background.
Suggested Control Mice:
- Wildtype littermates
Research Applications
Strain Origin
Donor: Bruce Beutler, M.D. The Scripps Research Institute.
Primary Reference:
- Blasius AL, Arnold CN, Georgel P, Rutschmann S, Xia Y, Lin P, Ross C, Li X, Smart NG, Beutler B. Slc15a4, AP-3, and Hermansky-Pudlak syndrome proteins are required for Toll-like receptor signaling in plasmacytoid dendritic cells, Proc Natl Acad Sci U S A 2010;107(46):19973-8 (Medline PMID: 21045126)
- For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute, La Jolla, CA.
Special Considerations
Random intra-strain mating.
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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