Strain Name: B6.129P2-Gt(ROSA)26Sortm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk/Mmmh
Stock Number: 034320-MU
Other Names:
Gene Details: (provided by MGI)
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Target: Gt(ROSA)26Sortm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk Name: Alteration at locus: Knock-In |
Genetic Alterations:
E14Tg2A.4 mouse embryonic stem cells were electroporated with a Rosa26 vector carrying the combined EF1a-tTA and TetO-mCh-Rs1 transgenes in a single construct. ES cells remain on the 129P2/OlaHsd background.
Genotype Determination:
ES Cell Line: E14 derived from B6.129P2
Strain Description
Phenotype
Hemizygous mice have increased mineralization in calvaria.
Founder genetic background: 129P2/OlaHsd
Strain genetic background: B6.129P2
Strain Development: E14Tg2A.4 mouse embryonic stem cells were electroporated with a Rosa26 vector carrying the combined EF1a-tTA and TetO-mCh-Rs1 transgenes in a single construct. Mice were generated by blastocyst injection, and chimeras were backcrossed to C57BL/6.
Suggested Control Mice: Wild-type littermates or C57BL/6
Research Applications
- Cell Biology
- Developmental Biology
- Endocrine Deficiency
- Metabolism
Strain Origin
Donor: Bruce Conklin, M.D., Gladstone Institute of Cardiovascular Disease
Primary Reference:
Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Stem Cell Res Ther. 2011 Mar 4;2(2):11. (Medline PMID: 21375737).
Colony and Husbandry Information
Appearance
Coat color: Black
Other:
Breeding
MMRRC Breeding System: Backcross
Breeding Scheme(s):
- C57BL/6 female x Hemizygous male or reciprocol mating.
- Note: Donor reports that attemts to backross to 129P2/OlaHsd produced poor litter sizes.
Generation: N6
Overall Breeding Performance: Good
Reproductive Statistics
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
| Viability and Fertility: | Female | Male | |
|---|---|---|---|
| Homozygotes are viable: | Unknown | Unknown | |
| Homozygotes are fertile: | Unknown | Unknown | |
| Hetero/Hemizygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size: 7
Recommended wean age: 3 weeks
Special Considerations
Doxycycline diet = BioServ DoxDiet 200 mg/kg. No embryonic lethality was identifed when mated off of doxycycline (with transgenes induced). However, the donor recommends maintining the mouse line on doxycycline (or maintaining the ES cell line in doxy-containing media) to prevent any unexpected effects from Rs1 expression.
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
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Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
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| Aliquot | Approximate quantity.2 | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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