Strain Detail Sheet

Strain Name: B6;129S4-Myh9^{tm2(GFP/MYH10)Rsad}/Mmnc

Stock Number: 034321-UNC

Other Names:

Gene Details: (provided by MGI)

Target: Myh9^{tm2(GFP/MYH10)Rsad}
Name: targeted mutation 2, Robert S Adelstein
Alteration at locus: Knock-In

Genetic Alterations:
cDNA encoding GFP- nonmuscle myosin IIB heavy chain was inserted into exon2 of the nonmuscle myosin IIA heavy chain (Myh9) gene locus, placing nonmuscle myosin IIB under control of the IIA promoter, expressing GFP-IIB in place of IIA and ablating IIA

Genotype Determination:

The genotyping protocol for this strain is currently not available. The MMRRC will develop a protocol if this strain is ordered.

ES Cell Line: v6.5

Strain Description

Phenotype
Homozygous phenotype: Homozygous mice die at E10.5-11.5 with defects in placenta formation and angiogenesis.

Heterozygous phenotype: Heterozygous mice are normal for all tested phenotypes and are fertile.

Mammalian Phenotype Terms:(provided by MGI)

Extend all MPTs
assigned by genotype

Myh9^{tm2(GFP/MYH10)Rsad}/Myh9^{tm2(GFP/MYH10)Rsad}

involves: 129S4/SvJae * C57BL/6

mortality/aging
- complete embryonic lethality during organogenesis (MGI Ref ID J:163702)
  - die between E9.5 and E10.5
embryogenesis phenotype
- embryogenesis phenotype (MGI Ref ID J:163702)
  - unlike null mice, knock in embryos undergo gastrulation
  - abnormal placenta morphology (MGI Ref ID J:163702)
    - placentas are composed predominantly of clusters of cuboidal trophoblasts
    - abnormal placenta labyrinth morphology (MGI Ref ID J:163702)
      - the labyrinth layer is difficult to discern
      - abnormal placental labyrinth vasculature morphology (MGI Ref ID J:163702)
        
        embryonic blood vessels fail to invade the labyrinth layer
    - abnormal placenta size (MGI Ref ID J:163702)
      - thinner and more compact compared to controls
    - abnormal spongiotrophoblast layer morphology (MGI Ref ID J:163702)
      - the spongiotrophoblast layer is difficult to discern
  - abnormal vitelline vasculature morphology (MGI Ref ID J:163702)
    - fewer visible vessels at E9.5 and E10.5
  - embryonic growth retardation (MGI Ref ID J:163702)
    - display growth retardation that becomes more pronounced with age
  - pale yolk sac (MGI Ref ID J:163702)
    - at E9.5 and E10.5
cardiovascular system phenotype
- abnormal brain vasculature morphology (MGI Ref ID J:163702)
  - the vascular network is less intricate at E10.5
- abnormal vascular branching morphogenesis (MGI Ref ID J:163702)
  - the vascular networks of the head and trunk are less intricate at E10.5
- abnormal vitelline vasculature morphology (MGI Ref ID J:163702)
  - fewer visible vessels at E9.5 and E10.5
cellular phenotype
- abnormal cell adhesion (MGI Ref ID J:163702)
  - fewer and smaller focal adhesions are detected in MEFs and the stress fibers in the focal adhesions are in disarray
- abnormal cell content or morphology (MGI Ref ID J:163702)
  - in MEFs actin filaments are thin, disorganized and often fail to align with the direction of migration
- abnormal cell migration (MGI Ref ID J:163702)
  - in a wound healing assay MEFs close the wound more rapidly compared to wild-type controls
  - a transwell assay indicates that directional cell migration is impaired in MEFs
growth/size phenotype
- embryonic growth retardation (MGI Ref ID J:163702)
  - display growth retarda tion that becomes more pronounced with age
nervous system phenotype
- abnormal brain vasculature morphology (MGI Ref ID J:163702)
  - the vascular network is less intricate at E10.5

Strain genetic background: B6;129

Strain Development: 129 embryonic stem cells were injected into C57BL/6 blastocysts and chimeras were born. Chimeras were mated to C57BL/6 wild type mice to generate heterozygotes. Strain is maintained as heterozygotes.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC Strain		Reference Strains		Genomic makeup (Mb)
Strain Name	MMRRC ID #	Reference 1	Reference 2	Ref 1	Ref 2	Het	Unknown
B6;129S4-Myh9^{tm2(GFP/MYH10)Rsad}/Mmnc	MMRRC:034321-UNC	C57BL/6J	129S4/Ak7	1653	0	931	0

Suggested Control Mice:

Wildtype littermates

Research Applications

Cell Biology
Developmental Biology
Reproduction
Research Tools

Strain Origin

Donor: Robert S. Adelstein, M.D., NIH/NHLBI

Primary Reference:

Wang A; Ma X; Conti MA; Liu C; Kawamoto S; Adelstein RS, Nonmuscle myosin II isoform and domain specificity during early mouse development., Proc Natl Acad Sci U S A 2010 Aug 17;107(33):14645-50 (Medline PMID: 20679233)

Special Considerations

Strain is maintained as heterozygotes.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level:

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution:

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees:

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

- Products for this strain are Not Yet Available for Ordering -
If you register interest in this strain, you will be notified when it becomes available for ordering.

MMRRC Item #	Description

Distribution
Fee/unit (US $)

Units

Notes

034321-UNC-RESUS

Litter recovered from cryo-archive

$2,022.00

Non-Profit

Litter

Recovered litter¹; additional fees for any special requests.

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).