Name: targeted mutation 3, Robert S Adelstein
Alteration at locus: Knock-In
cDNA encoding GFP- nonmuscle myosin IIA head fused to IIB rod was inserted into exon2 of the nonmuscle myosin IIA heavy chain (Myh9) gene locus, placing a chimeric nonmuscle myosin IIA/B under control of the IIA promoter. Neo cassette was placed into intron.
ES Cell Line: v6.5
Homozygous phenotype: Homozygous mice die at E10.5-11.5 with defects in placenta formation and angiogenesis.
Heterozygous phenotype: Heterozygous mice are normal for all tested phenotypes and are fertile.
Strain genetic background: B6;129
Strain Development: V6.5 embryonic stem cells were injected
into C57BL/6 blastocysts and chimeras were born. Chimeras were mated to
C57BL/6 wild type mice to generate heterozygotes. Strain is maintained as
Strain Genetic Background Validation:
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
|MMRRC Strain||Reference Strains||Genomic makeup (Mb)|
|Strain Name||MMRRC ID #||Reference 1||Reference 2||Ref 1||Ref 2||Het||Unknown|
Suggested Control Mice:
Donor: Robert S. Adelstein, M.D., NIH/NHLBI
Wang A; Ma X; Conti MA; Liu C; Kawamoto S; Adelstein RS, Nonmuscle myosin II isoform and domain specificity during early mouse development., Proc Natl Acad Sci U S A 2010 Aug 17;107(33):14645-50 (Medline PMID: 20679233)
Strain is maintained as heterozygotes.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email firstname.lastname@example.org.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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If you register interest in this strain, you will be notified when it becomes available for ordering.
Fee/unit (US $)
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).
The MMRRC is a collaborative effort, funded by grants from the NIH