Strain Name:
BLSW/Ntra/Mmmh
Stock Number:
034409-MU
Citation ID:
RRID:MMRRC_034409-MU

Strain Information

Gipc3ahl5-Black Swiss
Name: GIPC PDZ domain containing family, member 3; age related hearing loss 5-Black Swiss
Synonyms: Gipc3343A
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Spontaneous Mutation
Gipc3
Name: GIPC PDZ domain containing family, member 3
Synonyms: Gipc3, Rgs19ip3, Ahl5
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Spontaneous Mutation
NCBI: 209047
Homologene: 77068
Genetic Alterations
BLSW/Ntra carries a spontaneous mutation initially identified as age related hearing loss 5 (ahl5), which confers hearing impairment. The mutation is located in the Gipc3 gene and is a G>A transition at nucleotide position c.343. This allele is a strain characteristic of BLSW/Ntra.
Genotype Determination
Phenotype
Homozygous: Thr ahl5 alle is an amino acid replacement (glycine to arginine) in the PDZ domain of Gipc3. It causes progressive sensorineural hearing loss and audiogenic seizures in young adults.

Heterozygous: The strain is not available as heterozygous mutation.
Mammalian Phenotype Terms
Allelic Composition: Gipc3ahl5-BlackSwiss/Gipc3ahl5-BlackSwiss (Genetic Background: involves: Black Swiss * CAST/EiJ )

MeSH Terms
  • Age Factors
  • Analysis of Variance
  • Animals
  • Animals, Outbred Strains
  • Auditory Threshold/physiology
  • Cadherins/genetics
  • Chromosome Mapping/methods
  • Cluster Analysis
  • Evoked Potentials, Auditory, Brain Stem/physiology
  • Genetic Linkage
  • Genetic Predisposition to Disease
  • Hearing Loss, Sensorineural/genetics
  • Mice
  • Normal Distribution
  • Polymorphism, Single Nucleotide
  • Protein Precursors/genetics
  • Quantitative Trait, Heritable
  • Acoustic Stimulation
  • Carrier Proteins/genetics
  • Crosses, Genetic
  • DNA Mutational Analysis
  • Genetic Predisposition to Disease/genetics
  • Hair Cells, Auditory/metabolism
  • Hearing Tests
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Mechanotransduction, Cellular/genetics
  • Mice, Transgenic
  • Mutation, Missense/genetics
  • Antigens, CD
  • Antigens, Neoplasm
  • Antigens, Surface
  • Auditory Threshold
  • Avian Proteins
  • Basigin
  • Blood Proteins
  • Disease Models, Animal
  • Epilepsy, Reflex/chemically induced
  • Epilepsy, Reflex/genetics
  • Evoked Potentials, Auditory, Brain Stem/genetics
  • Gene Expression Regulation, Developmental
  • Genes, Recessive
  • Genotype
  • Membrane Glycoproteins/genetics
  • Mice, Knockout
  • Mice, Mutant Strains
  • Microsatellite Repeats/genetics
  • N-Methylaspartate
  • Physical Chromosome Mapping
  • Picrotoxin
  • Vesicular Transport Proteins
Strain Development
Black Swiss mice were initially obtained from Taconic. By strict brother x sister matings the current F11 population was obtained.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Models for Human Disease
  • Neurobiology
  • Sensorineural
Donor
Konrad Noben-Trauth, Ph.D., National Institute on Deafness and Other Communication Disorders/National Institutes of Health 
Primary Reference
  • Drayton M; Noben-Trauth K, Mapping quantitative trait loci for hearing loss in Black Swiss mice., Hear Res. 2006 Feb;212(1-2):128-39 (Medline PMID: 16426780)
  • Charizopoulou N, Lelli A, Schraders M, Ray K, Hildebrand MS, Ramesh A, Srisailapathy CR, Oostrik J, Admiraal RJ, Neely HR, Latoche JR, Smith RJ, Northup JK, Kremer H, Holt JR, Noben-Trauth K.,Gipc3 mutations associated with audiogenic seizures and sensorineural hearing loss in mouse and human. Nat Commun. 2011 Feb;2:201. (Medline PMID: 21326233)
  • Misawa H; Sherr EH; Lee DJ; Chetkovich DM; Tan A; Schreiner CE; Bredt DS, Identification of a Monogenic Locus (jams1) Causing Juvenile Audiogenic Seizures in Mice., J Neurosci. 2002 Dec 1;22(23):10088-93 (Medline PMID: 12451109)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034409-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
034409-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034409-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.