Strain Detail Sheet

Strain Name: BLSW/Ntra/Mmmh

Stock Number: 034409-MU

Other Names:

Gene Details: (provided by MGI)

Allele Symbol: Gipc3ahl5-Black Swiss
Name: age related hearing loss 5-Black Swiss
Alteration at locus: Spontaneous Mutation

Genetic Alterations:
BLSW/Ntra carries a spontaneous mutation initially identified as age related hearing loss 5 (ahl5), which confers hearing impairment. The mutation is located in the Gipc3 gene and is a G>A transition at nucleotide position c.343. This allele is a strain characteristic of BLSW/Ntra.

Genotype Determination:

    ES Cell Line: Not applicable

    Strain Description

    Phenotype
    Homozygous phenotype: Thr ahl5 alle is an amino acid replacement (glycine to arginine) in the PDZ domain of Gipc3. It causes progressive sensorineural hearing loss and audiogenic seizures in young adults.

    Heterozygous phenotype: The strain is not available as heterozygous mutation.

    Founder genetic background: Black Swiss

    Strain genetic background: Black Swiss

    Strain Development: Black Swiss mice were initially obtained from Taconic. By strict brother x sister matings the current F11 population was obtained.

    Suggested Control Mice:

    • Wildtype littermates

    Research Applications

    • Cell Biology
    • Models for Human Disease
    • Neurobiology
    • Sensorineural

    Strain Origin

    Donor: Konrad Noben-Trauth, PhD, NIDCD/NIH

    Primary Reference:

    • Drayton M; Noben-Trauth K, Mapping quantitative trait loci for hearing loss in Black Swiss mice., Hear Res 2006 Feb;212(1-2):128-39 (Medline PMID: 16426780)
    • Charizopoulou N, Lelli A, Schraders M, Ray K, Hildebrand MS, Ramesh A, Srisailapathy CR, Oostrik J, Admiraal RJ, Neely HR, Latoche JR, Smith RJ, Northup JK, Kremer H, Holt JR, Noben-Trauth K.,Gipc3 mutations associated with audiogenic seizures and sensorineural hearing loss in mouse and human. Nat Commun. 2011 Feb;2:201. (Medline PMID: 21326233)
    • Misawa H; Sherr EH; Lee DJ; Chetkovich DM; Tan A; Schreiner CE; Bredt DS, Identification of a Monogenic Locus (jams1) Causing Juvenile Audiogenic Seizures in Mice., J Neurosci 2002 Dec 1;22(23):10088-93 (Medline PMID: 12451109)

    Special Considerations

    None

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

    Order Request Information

    Availability Level:

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution:

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees:

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    		Distribution
    Fee/unit (US $)    		 Units Notes
    034409-MU-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    		 Litter Recovered litter1; additional fees for any special requests.
    034409-MU-SPERMCryo-preserved spermatozoa
    $437.00 / $817.00
    Non-Profit / For-Profit
    		 Aliquot Approximate quantity.2
    034409-MU-EMBRYOCryo-preserved embryos
    $1,038.00 / $2,621.00
    Non-Profit / For-Profit
    		 Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

    The MMRRC is a collaborative effort, funded by grants from the NIH

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    Generated: 05/23/2013