Strain Name: B6.Cg-Retntm1Laz Tg(CD68-RETN)#Laz/Mmmh
Stock Number: 034624-MU
Other Names:
Gene Details: (provided by MGI)
|
Allele Symbol: Retntm1Laz Name: targeted mutation 1, Mitchell A Lazar Alteration at locus: Targeted Mutation |
|
Transgene: Tg(CD68-RETN)#Laz Name: transgene insertion, Mitchell A Lazar Alteration at locus: Transgenic |
|
Promoter: CD68 (Human) Name: CD68 molecule Alteration at locus: Transgenic |
|
Transgene: RETN (Human) Name: resistin Alteration at locus: Transgenic |
Genetic Alterations:
Transgenic mice were derived on the C57BL/6 background with macrophage- specific expression of human resistin using the human CD68 promoter. Several independent lines were generated, 2 of which were established and bred to C57BL/6 Retn-/- mice to generate mice that express human resistin but lack any expression of murine resistin.
Genotype Determination:
ES Cell Line: Not Applicable
Strain Description
Phenotype
Human resistin expressed but no expression of murine resistin.
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Retn/Retn Tg(CD68-RETN)#Laz/?
involves: 129S6/SvEvTac * C57BL/6 - adipose tissue phenotype
- abnormal white adipose tissue physiology (MGI Ref ID J:171262)
- mice exhibit an increase in inflammatory markers (Tnfa, Il6, Il1b, Cxcl15, Cd68) and an accumulation of macrophages in WAT
- abnormal white adipose tissue physiology (MGI Ref ID J:171262)
- homeostasis/metabolism phenotype
- enhanced lipolysis (MGI Ref ID J:171262)
- impaired glucose tolerance (MGI Ref ID J:171262)
- on a high fat diet
- increased circulating free fatty acid level (MGI Ref ID J:171262)
- mice on a high fat diet exhibit significantly increased free fatty acid levels
- increased circulating glycerol level (MGI Ref ID J:171262)
- mice on a high fat diet exhibit significantly increased glycerol levels
- increased circulating insulin level (MGI Ref ID J:171262)
- after 2-3 weeks on a high fat (60%) diet, male mice exhibit increased serum insulin, but not glucose, levels
- increased triglyceride level (MGI Ref ID J:171262)
- mice exhibit a 52% increase in muscle triglyceride levels on a high fat diet
- insulin resistance (MGI Ref ID J:171262)
- glucose-infusion rate is 39% lower than controls
- decrease in insulin sensitivity as determined by insulin tolerance tests
- decreased glucose disposal rate
- insulin s timulated glucose uptake in skeletal muscle and white adipose tissue is decreased (respectively 56% and 22%) compared to Retn-/- controls
Strain genetic background: C57BL/6
Strain Development: Transgenic mice were derived on the C57BL/6 background with macrophage-specific expression of human resistin using the human CD68 promoter. Several independent lines were generated, 2 of which were established and bred to C57BL/6 Retn-/- mice to generate mice that express human resistin but lack any expression of murine resistin.
Suggested Control Mice:
- Wild-type littermates homozygous for the KO and wild-type for the Tg.
Research Applications
- Cardiovascular
- Cell Biology
- Diabetes/Obesity
- Immunology and Inflammation
- Metabolism
- Models for Human Disease
Strain Origin
Donor: Dr. Mitchell Lazar, University Of Pennsylvania
Primary Reference:
Qatanani M, Szwergold NR, Greaves DR, Ahima RS, Lazar MA. Macrophage-derived human resistin exacerbates adipose tissue inflammation and insulin resistance in mice. J Clin Invest. 2009 Mar;119(3):531-9. (Medline PMID: 19188682).
Colony and Husbandry Information
Appearance
Coat color: Black
Other:
Breeding
MMRRC Breeding System: Random intra-strain mating
Breeding Scheme(s):
- Homozygous KO, Wild-type Tg female x Homozygous KO, Hemizygous Tg
- male
Generation:
Overall Breeding Performance: Good
Reproductive Statistics
| Viability and Fertility: | Female | Male | |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | Mice that are homozygous for the KO and hemizygous for the Tg are viable |
| Homozygotes are fertile: | |||
| Heterozygotes are fertile: | Yes | Yes | Mice that are homozygous for the KO and hemizygous for the Tg are fertile |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size: 8
Recommended wean age: 3 weeks
Special Considerations
None
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
|
Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
|
| Litter | Recovered litter1; additional fees for any special requests. | ||||
|
| Aliquot | Approximate quantity.2 | ||||
|
| Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.