Strain Name: B6;FVB-Tg(Grm2-cre,-EGFP)631Lfr/Mmucd
Stock Number: 034766-UCD
Other Names:
- mGluR2-Cre-IRES-eGFP, B6;FVB-Tg(Grm2-cre,-EGFP)631Lfr/Mmcd
Gene Details: (provided by MGI)
|
Transgene: Tg(Grm2-cre,-EGFP)631Lfr Name: transgene insertion 631, Louis F Reichardt Alteration at locus: Transgenic (cre) |
|
Transgene: cre (Bacteriophage P1) Name: cre recombinase Alteration at locus: Transgenic (cre) |
|
Reporter: EGFP (Jelly Fish) Name: Enhanced Green Fluorescent Protein Alteration at locus: Transgenic (cre) |
|
Promoter: Grm2 Name: glutamate receptor, metabotropic 2 Alteration at locus: Transgenic (cre) |
Genetic Alterations:
Cre recombinase with a nuclear localization signal followed by an IRES-EGFP sequence was cloned into a plasmid vector containing 18 kb of 5' upstream Gria2 sequence including the first two exons. The excised expression cassette injected into mouse pronuclei to generate transgenic animals.
Genotype Determination:
ES Cell Line: Not Applicable
Strain Description
Phenotype
Homozygous phenotype: None.
Hemizygous phenotype: None.Useful for examining gene function in mouse models of diseases that affect > cortex, hippocampus or cerebellum
Strain genetic background: C57BL/6
Strain Development: Allele has been crossed mainly with C57BL/6. Maintained as hets.
Suggested Control Mice:
- Wild-type littermates
Research Applications
- Cell Biology
- Developmental Biology
- Neurobiology
Strain Origin
Donor: Louis Reichardt, Ph.D., University of California, San Francisco
Primary Reference:
Chen AI, Nguyen CN, Copenhagen DR, Badurek S, Minichiello L, Ranscht B, Reichardt LF. TrkB (tropomyosin-related kinase B) controls the assembly and maintenance of GABAergic synapses in the cerebellar cortex. J Neurosci. 2011 Feb 23;31(8):2769-80 (Medline PMID: 21414899)
Colony and Husbandry Information
Appearance
Coat color: Black or agouti
Other:
Breeding
MMRRC Breeding System:
Breeding Scheme(s):
Generation:
Overall Breeding Performance: Excellent
Reproductive Statistics
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
| Viability and Fertility: | Female | Male | |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | |
| Homozygotes are fertile: | Yes | Yes | |
| Hetero/Hemizygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size:
Recommended wean age: 3 weeks
Special Considerations
None
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
|
Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
|
| Litter | Recovered litter1; additional fees for any special requests. | ||||
|
| Aliquot | Approximate quantity.2 | ||||
|
| Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.