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Availability & Fees Order this Strain
Homozygous: There is abnormal ear morphology detected. There is bidirectional circling behavior and hyperactivity detected.
Heterozygous: No observed phenotype.
CSS-14 was generated through 5 generations of marker-assisted backcrossing at Charles River Laboratories. At each generation, optimal breeders were selected from among all offspring carrying a non-recombinant SWR/J chromosome 14, and backcrossed to C57BL/10J. Additional backcrossing was then carried out in the donor's colony. Genome-wide SNP genotyping subsequently confirmed that the CSS-14 genetic background was 100% C57BL/10J-derived and that the introgressed SWR/J chromosome 14 encompassed a minimal interval of 92.8 Mb, extending from dbSNP rs3710916 (8.8 Mb) to rs6179144 (101.6 Mb). Offspring of Chr14SWR/SWR X Chr14B10/SWR matings were phenotyped as circling or non-circling and genotyped at markers across chromosome 14. In this strain, the region of SWR/J homozygosity on chromosome 14 has been narrowed to 8.4Mb through breeding and mapping strategies performed during the positional cloning the circling phenotype.
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
Roberts KA, Abraira VE, Tucker AF, Goodrich LV, Andrews NC. Mutation of Rubie, a novel long non-coding RNA located upstream of Bmp4, causes vestibular malformation in mice. PLoS One. 2012;7(1):e29495. Epub 2012 Jan 12. (Mediline PMID: 22253730).
Doolittle DP. Two-gene circling in the mouse. Genetics 48(7):887
Colony Surveillance Program and Current Health Reports
Homozygous female x Homozygous male
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.