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Availability & Fees Order this Strain
Homozygous: There is impaired granulosa cell differentiation, decreased primary ovarian follicle number, ovary atrophy, decreased oocyte number, and female infertility.
Heterozygous: Normal
A targeting construct was prepared and exon 1 of the mouse Sohlh2 gene was replaced with a neomycin-resistance gene flanked by 5.0 kb (50) and 5.0 kb (30) of homologous DNA sequences. The vector was linearized and electroporated into embryonic stem cells (AB2.1). The embryonic stem cells, identified as heterozygous for the targeted Sohlh2 mutation, were microinjected into the C57BL/6 blastocysts to produce chimeric mice that carried the mutation into the germline. These mice were mated with C57BL/6 wild-type (WT) mice to generate Sohlh2 heterozygous animals that were subsequently crossed to produce offspring from the second generation for analysis.
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
Choi Y, Yuan D, Rajkovic A; Germ cell-specific transcriptional regulator sohlh2 is essential for early mouse folliculogenesis and oocyte-specific gene expression; Biol Reprod; 2008 Dec;79(6):1176-82, Epub 2008 Aug 27; (Medline PMID:18753606).
Suzuki H, Ahn HW, Chu T, Bowden W, Gassei K, Orwig K, Rajkovic A; SOHLH1 and SOHLH2 coordinate spermatogonial differentiation; Dev Biol; 2012 Jan 15;361(2):301-12, Epub 2011 Oct 26; (Medline PMID:22056784).
Colony Surveillance Program and Current Health Reports
C57BL/6 female x Heterozygous male or reciprocal mating
Heterozygous female x Heterozygous male sibling
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.