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Availability & Fees Order this Strain
Homozygous: Mice homozygous for this targeted replacement of the mouse gene are viable and fertile. There is an abnomal enlarge lymph nodes physiology detected [MP:0000702] when the mice are 4-5 month old (Related to MRL/MpJ background) There is an abnomal skin dermitis physiology detected when the mice are 4-5 month old (Related to MRL/MpJ background).
Heterozygous: Mice heterozygous for this targeted replacement of the mouse gene are viable and fertile. There is an abnomal enlarge lymph nodes physiology detected [MP:0000702] when the mice are 4-5 month old(Related to MRL/MpJ background). There is an abnomal skin dermitis physiology detected when the mice are 4-5 month old (Related to MRL/MpJ background).
The targeted vector was electroporated into the 129/Sv-derived ES cells. The mutant mice were crossed to a C57BL/6 background EIIA-Cre strain to remove the floxed neomycin cassette. This strain was maintained on a mixed 129 and C57BL/6 background (B6;129-IgkCtm1(IGKC)Mnz/J). Then, this strain was backcrossed to MRL/MpJ mice for 6 generations.
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
Andrews SF, Zhang Q, Lim S, Li L, Lee JH, Zheng NY, Huang M, Taylor WM, Farris AD, Ni D, Meng W, Luning Prak ET, Wilson PC. Global analysis of B cell selection using an immunoglobulin light chain-mediated model of autoreactivity. J Exp Med. 2013 Jan 14;210(1):125-42. (Medline PMID: 23267014)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.