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Strain Name:
C57BL/6J-MtgxR0041Btlr/Mmmh
Stock Number:
038335-MU
Citation ID:
RRID:MMRRC_038335-MU
Other Names:
R0041 (G1), C57BL/6J-MtgxR0041Btlr
Major Collection:

Strain Information

Nefh
Name: neurofilament, heavy polypeptide
Synonyms: NF-H, NF200, NEFH
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380684
HGNC: HGNC:7737
Homologene: 40755
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Rnf220
Name: ring finger protein 220
Synonyms: 5730503K05Rik, 4931406I20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66743
Homologene: 10036
Pck1
Name: phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms: Pck-1, PEPCK
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18534
HGNC: HGNC:8724
Homologene: 1944
Rlf
Name: rearranged L-myc fusion sequence
Synonyms: 9230110M18Rik, MommeD8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109263
Homologene: 8243
Swap70
Name: SWA-70 protein
Synonyms: 70kDa
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20947
Homologene: 7557
Braf
Name: Braf transforming gene
Synonyms: Braf-2, Braf2, 9930012E13Rik, D6Ertd631e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,344,628 bp
  • A to G, chromosome 1 at 60,281,871 bp
  • A to C, chromosome 1 at 87,378,976 bp
  • G to A, chromosome 1 at 175,726,471 bp
  • G to T, chromosome 1 at 189,295,691 bp
  • A to G, chromosome 2 at 26,911,551 bp
  • A to G, chromosome 2 at 26,983,974 bp
  • T to A, chromosome 2 at 35,085,721 bp
  • A to G, chromosome 2 at 150,323,825 bp
  • A to G, chromosome 2 at 173,155,210 bp
  • A to G, chromosome 3 at 88,747,903 bp
  • A to G, chromosome 3 at 88,910,235 bp
  • T to C, chromosome 3 at 125,915,090 bp
  • G to A, chromosome 3 at 158,164,260 bp
  • G to C, chromosome 4 at 62,486,554 bp
  • A to G, chromosome 4 at 117,273,284 bp
  • T to A, chromosome 4 at 121,149,929 bp
  • A to G, chromosome 5 at 49,960,559 bp
  • T to A, chromosome 5 at 89,684,467 bp
  • T to A, chromosome 5 at 129,874,262 bp
  • C to T, chromosome 6 at 39,640,479 bp
  • A to T, chromosome 6 at 40,926,108 bp
  • G to A, chromosome 6 at 116,541,334 bp
  • T to A, chromosome 6 at 118,594,027 bp
  • A to G, chromosome 6 at 141,918,459 bp
  • A to G, chromosome 7 at 45,304,946 bp
  • T to A, chromosome 7 at 62,463,560 bp
  • A to T, chromosome 7 at 107,590,186 bp
  • A to G, chromosome 7 at 110,279,355 bp
  • A to T, chromosome 8 at 31,738,663 bp
  • T to A, chromosome 9 at 39,461,476 bp
  • T to C, chromosome 9 at 44,683,613 bp
  • T to G, chromosome 9 at 63,145,851 bp
  • T to A, chromosome 9 at 109,448,164 bp
  • T to A, chromosome 9 at 111,029,568 bp
  • T to A, chromosome 10 at 3,964,074 bp
  • T to A, chromosome 10 at 3,964,076 bp
  • A to G, chromosome 10 at 99,108,512 bp
  • A to G, chromosome 10 at 127,286,872 bp
  • C to T, chromosome 11 at 4,945,184 bp
  • G to T, chromosome 11 at 59,043,977 bp
  • T to A, chromosome 11 at 61,452,355 bp
  • A to C, chromosome 11 at 67,209,078 bp
  • A to T, chromosome 11 at 68,954,516 bp
  • A to G, chromosome 11 at 119,402,575 bp
  • T to A, chromosome 12 at 65,106,443 bp
  • G to C, chromosome 12 at 109,455,513 bp
  • A to G, chromosome 13 at 33,667,632 bp
  • A to T, chromosome 13 at 54,726,838 bp
  • T to A, chromosome 13 at 89,661,985 bp
  • T to C, chromosome 14 at 69,510,477 bp
  • C to A, chromosome 15 at 101,739,318 bp
  • T to C, chromosome 16 at 32,256,655 bp
  • A to T, chromosome 16 at 91,659,333 bp
  • C to A, chromosome 17 at 57,876,469 bp
  • A to T, chromosome 17 at 62,607,472 bp
  • T to C, chromosome 18 at 10,140,240 bp
  • C to T, chromosome 18 at 23,646,875 bp
  • A to G, chromosome 18 at 70,242,034 bp
  • A to T, chromosome 18 at 73,935,038 bp
  • C to A, chromosome 19 at 42,123,784 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0041 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038335-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.