Strain Name:
C57BL/6J-MtgxR0565Btlr/Mmmh
Stock Number:
038756-MU
Citation ID:
RRID:MMRRC_038756-MU
Other Names:
R0565 (G1), C57BL/6J-MtgxR0565Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Ptch2
Name: patched 2
Synonyms: ptc2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19207
HGNC: HGNC:9586
Homologene: 37842
Sstr2
Name: somatostatin receptor 2
Synonyms: SSTR-2, sst2, Smstr2, Smstr-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20606
Homologene: 37427
Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
L3mbtl2
Name: L3MBTL2 polycomb repressive complex 1 subunit
Synonyms: 4732493N06Rik, m4mbt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 214669
Homologene: 12882
Ireb2
Name: iron responsive element binding protein 2
Synonyms: Irp2, D9Ertd85e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 64602
VEGA: 9
HGNC: HGNC:6115
Homologene: 11280
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Cep131
Name: centrosomal protein 131
Synonyms: AZ1, Azi1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12009
Homologene: 7638
Sel1l
Name: sel-1 suppressor of lin-12-like (C. elegans)
Synonyms: Sel1h
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20338
Homologene: 31286
Ranbp2
Name: RAN binding protein 2
Synonyms: A430087B05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19386
VEGA: 10
Homologene: 87808
Ipo7
Name: importin 7
Synonyms: RanBP7, Imp7, A330055O14Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233726
HGNC: HGNC:9852
Homologene: 4659
Dhx40
Name: DEAH-box helicase 40
Synonyms: ARG147, DDX40, 2410016C14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67487
Homologene: 57000
Cep350
Name: centrosomal protein 350
Synonyms: 6430546F08Rik, 4933409L06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74081
Homologene: 8879
Ipo8
Name: importin 8
Synonyms: OM-1, 6230418K12Rik, Om1, C130009K11Rik, Ranbp8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320727
HGNC: HGNC:9853
Homologene: 48430
Immt
Name: inner membrane protein, mitochondrial
Synonyms: P87/89, P89, P87, HMP, 1700082C19Rik, D830041H16Rik, mitofilin, Micos60
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 76614
HGNC: HGNC:6047
Homologene: 38234
Ncapd3
Name: non-SMC condensin II complex, subunit D3
Synonyms: 4632407J06Rik, 2810487N22Rik, B130055D15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 78658
VEGA: 9
Homologene: 41021
Sphk1
Name: sphingosine kinase 1
Synonyms: 1110006G24Rik, SK1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20698
Homologene: 39748
Mmp16
Name: matrix metallopeptidase 16
Synonyms: Membrane type 3-MMP, MT3-MMP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17389
HGNC: HGNC:7162
Homologene: 55939
Cul7
Name: cullin 7
Synonyms: p193, 2510004L20Rik, p185, C230011P08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66515
Homologene: 56683
Kcnj3
Name: potassium inwardly-rectifying channel, subfamily J, member 3
Synonyms: Kir3.1, GIRK1, Kcnf3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16519
HGNC: HGNC:6264
Homologene: 1687
Nefm
Name: neurofilament, medium polypeptide
Synonyms: NF160, Nfm, NF-M, NF165, Nef3, neurofilament-M
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18040
HGNC: HGNC:7734
Homologene: 137211
Cemip
Name: cell migration inducing protein, hyaluronan binding
Synonyms: 12H19.01.T7, 6330404C01Rik, 9930013L23Rik, Hybid
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80982
Homologene: 10268
Wbp2
Name: WW domain binding protein 2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22378
Homologene: 32160
Psmd2
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Synonyms: TEG-190, Tex190, 9430095H01Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 21762
HGNC: HGNC:9559
Homologene: 2101
Ubr2
Name: ubiquitin protein ligase E3 component n-recognin 2
Synonyms: 9930021A08Rik, E130209G04Rik, ENSMUSG00000043296
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224826
VEGA: 17
Homologene: 26151
Ccdc88a
Name: coiled coil domain containing 88A
Synonyms: C330012F17Rik, C130096N06Rik, D130005J21Rik, GIV, HkRP1, Girdin, A430106J12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108686
Homologene: 41180
Trim11
Name: tripartite motif-containing 11
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 94091
Homologene: 14225
Pnpla7
Name: patatin-like phospholipase domain containing 7
Synonyms: E430013P11Rik, NRE
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241274
Homologene: 62431
Vit
Name: vitrin
Synonyms: 1700110E08Rik, 1700052E02Rik, 2810429K11Rik, AKH, akhirin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74199
VEGA: 17
Homologene: 24942
Lrrc8c
Name: leucine rich repeat containing 8 family, member C
Synonyms: E430036I04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100604
Homologene: 12997
Stxbp1
Name: syntaxin binding protein 1
Synonyms: Munc-18a, Sxtbp1, Munc18-1, Unc18h, Rb-sec1, N-sec1, nsec1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20910
Homologene: 2382
Wdr72
Name: WD repeat domain 72
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 546144
Homologene: 52326
Ethe1
Name: ethylmalonic encephalopathy 1
Synonyms: 0610025L15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66071
Homologene: 8622
Sec31b
Name: SEC31 homolog B, COPII coat complex component
Synonyms: LOC240667, Sec31l2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240667
Homologene: 56708
Mfsd12
Name: major facilitator superfamily domain containing 12
Synonyms: Wdt1, F630110N24Rik, gr
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 73822
Homologene: 45488
Spink12
Name: serine peptidase inhibitor, Kazal type 12
Synonyms: 9230117E20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 78242
Homologene: 87071
Cd180
Name: CD180 antigen
Synonyms: RP105, Ly78
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17079
HGNC: HGNC:6726
Homologene: 4077
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: A830008O07Rik, 1700010C24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70363
VEGA: 15
Homologene: 66605
Slc7a1
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
Synonyms: Rev-1, Rec-1, Atrc-1, Atrc1, 4831426K01Rik, mCAT-1, Cat1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11987
Homologene: 20658
Lipm
Name: lipase, family member M
Synonyms: 4632427C23Rik, Lipl3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 78753
VEGA: 19
Homologene: 28267
Vmn1r58
Name: vomeronasal 1 receptor 58
Synonyms: V3R4, V1rd4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 81014
Homologene: 41799
Pcdhb5
Name: protocadherin beta 5
Synonyms: Pcdhb4A, PcdhbE
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93876
HGNC: HGNC:8689
Homologene: 75103
Exoc3
Name: exocyst complex component 3
Synonyms: E430013E20Rik, Sec6l1, 2810050O03Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 211446
VEGA: 13
Homologene: 38296
Efna5
Name: ephrin A5
Synonyms: AL-1, RAGS, Ephrin-A5, Epl7, EFL-5, LERK-7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13640
HGNC: HGNC:3225
Homologene: 1482
Atosb
Name: atos homolog B
Synonyms: B230312A22Rik, Fam214b
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230088
Homologene: 32625
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: Ovos2, BC048546
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232400
Homologene: 45969
Irs2
Name: insulin receptor substrate 2
Synonyms: Irs-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 384783
HGNC: HGNC:6126
Homologene: 2778
4930562C15Rik
Name: RIKEN cDNA 4930562C15 gene
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78809
Homologene: 53527
Ppp1r15b
Name: protein phosphatase 1, regulatory subunit 15B
Synonyms: C530022L24Rik, 1810033K10Rik, CReP
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108954
Homologene: 13134
Cfap52
Name: cilia and flagella associated protein 52
Synonyms: 4933417B11Rik, 1700019F09Rik, Wdr16
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71860
Homologene: 12416
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: CPS, CPSase I, 4732433M03Rik, D1Ucla3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Or4c102
Name: olfactory receptor family 4 subfamily C member 102
Synonyms: GA_x6K02T2Q125-50079044-50079964, MOR237-2, Olfr1189
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258768
Homologene: 81569
Mertk
Name: MER proto-oncogene tyrosine kinase
Synonyms: Tyro 12, Nyk, Eyk, Mer, nmf12
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17289
HGNC: HGNC:7027
Homologene: 4626
Per3
Name: period circadian clock 3
Synonyms: mPer3, 2810049O06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18628
HGNC: HGNC:8847
Homologene: 7886
Caskin2
Name: CASK-interacting protein 2
Synonyms: 1600028L06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 140721
Homologene: 32485
Agtr1b
Name: angiotensin II receptor, type 1b
Synonyms: Agtr-1b, AT1B, Angtr-1b
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11608
HGNC: HGNC:336
Homologene: 84871
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: brm, Snf2l2, 2610209L14Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67155
Homologene: 2308
Fpr-rs3
Name: formyl peptide receptor, related sequence 3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14290
HGNC: HGNC:3827
Homologene: 130087
Upb1
Name: ureidopropionase, beta
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103149
Homologene: 9471
Rph3al
Name: rabphilin 3A-like (without C2 domains)
Synonyms: 6530413F01Rik, LOC385671, Noc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380714
Homologene: 5078
Amacr
Name: alpha-methylacyl-CoA racemase
Synonyms: 2-arylpropionyl-CoA epimerase, Macr1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17117
VEGA: 15
HGNC: HGNC:451
Homologene: 7410
Xkr8
Name: X-linked Kx blood group related 8
Synonyms: LOC381560, 4931440N07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381560
Homologene: 23068
Cabp5
Name: calcium binding protein 5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29865
Homologene: 8486
Lrfn3
Name: leucine rich repeat and fibronectin type III domain containing 3
Synonyms: A530045B06Rik, SALM4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233067
Homologene: 11558
Fndc9
Name: fibronectin type III domain containing 9
Synonyms: C030019I05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 320116
Homologene: 37404
Vps25
Name: vacuolar protein sorting 25
Synonyms: 1110020N13Rik, D11Wsu68e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 28084
Homologene: 6303
E330034G19Rik
Name: RIKEN cDNA E330034G19 gene
Synonyms: ZPAC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105418
Cd200l1
Name: CD200 molecule like 1
Synonyms: LOC208166, LOC385647, Gm609, iSEC1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208166
Homologene: 86707
Nt5c2
Name: 5'-nucleotidase, cytosolic II
Synonyms: cN-II, PNT5, NT5B
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 76952
HGNC: HGNC:8022
Homologene: 8158
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 67,166,449 bp
  • G to T, chromosome 1 at 133,136,653 bp
  • G to A, chromosome 1 at 155,961,195 bp
  • T to G, chromosome 2 at 24,980,117 bp
  • T to C, chromosome 2 at 32,819,848 bp
  • T to A, chromosome 2 at 55,595,264 bp
  • T to A, chromosome 2 at 88,592,009 bp
  • T to C, chromosome 2 at 128,771,483 bp
  • T to C, chromosome 3 at 20,315,674 bp
  • A to G, chromosome 4 at 17,987,705 bp
  • A to T, chromosome 4 at 43,034,647 bp
  • A to G, chromosome 4 at 117,106,143 bp
  • A to C, chromosome 4 at 132,730,917 bp
  • A to T, chromosome 4 at 151,033,952 bp
  • A to C, chromosome 5 at 105,607,028 bp
  • T to A, chromosome 5 at 148,352,069 bp
  • A to G, chromosome 5 at 150,980,944 bp
  • T to A, chromosome 6 at 71,846,483 bp
  • C to A, chromosome 6 at 128,568,743 bp
  • A to T, chromosome 6 at 148,786,723 bp
  • T to C, chromosome 7 at 5,411,166 bp
  • A to T, chromosome 7 at 13,401,335 bp
  • A to G, chromosome 7 at 24,607,889 bp
  • A to G, chromosome 7 at 30,360,791 bp
  • G to A, chromosome 7 at 83,964,110 bp
  • T to C, chromosome 7 at 110,049,593 bp
  • A to G, chromosome 8 at 11,004,592 bp
  • C to T, chromosome 9 at 27,087,998 bp
  • A to T, chromosome 9 at 54,899,983 bp
  • A to G, chromosome 9 at 74,217,306 bp
  • T to A, chromosome 9 at 75,180,112 bp
  • T to A, chromosome 10 at 58,476,336 bp
  • T to A, chromosome 10 at 75,428,354 bp
  • C to A, chromosome 10 at 81,361,409 bp
  • A to G, chromosome 11 at 29,461,042 bp
  • C to T, chromosome 11 at 46,238,157 bp
  • T to A, chromosome 11 at 58,990,584 bp
  • A to T, chromosome 11 at 67,949,599 bp
  • C to T, chromosome 11 at 75,833,401 bp
  • C to A, chromosome 11 at 86,771,167 bp
  • T to C, chromosome 11 at 101,258,905 bp
  • A to T, chromosome 11 at 113,625,619 bp
  • T to C, chromosome 11 at 115,801,016 bp
  • G to T, chromosome 11 at 116,082,385 bp
  • G to T, chromosome 11 at 116,536,358 bp
  • G to T, chromosome 11 at 120,073,762 bp
  • A to C, chromosome 12 at 31,298,915 bp
  • T to C, chromosome 12 at 91,811,889 bp
  • C to A, chromosome 12 at 91,813,945 bp
  • A to G, chromosome 13 at 74,182,275 bp
  • A to G, chromosome 13 at 102,702,874 bp
  • C to A, chromosome 14 at 24,306,917 bp
  • A to G, chromosome 14 at 68,124,621 bp
  • C to T, chromosome 15 at 10,981,946 bp
  • T to A, chromosome 15 at 71,490,837 bp
  • C to A, chromosome 15 at 81,684,286 bp
  • A to T, chromosome 16 at 4,864,336 bp
  • G to T, chromosome 16 at 20,660,426 bp
  • T to A, chromosome 16 at 45,444,173 bp
  • C to A, chromosome 16 at 87,416,010 bp
  • G to A, chromosome 17 at 20,624,021 bp
  • T to C, chromosome 17 at 46,652,003 bp
  • T to C, chromosome 17 at 46,955,886 bp
  • T to C, chromosome 17 at 62,881,036 bp
  • T to A, chromosome 17 at 78,624,837 bp
  • A to T, chromosome 18 at 12,759,444 bp
  • T to C, chromosome 18 at 37,320,767 bp
  • C to A, chromosome 18 at 44,104,688 bp
  • G to A, chromosome 19 at 26,681,875 bp
  • A to C, chromosome 19 at 34,116,506 bp
  • T to A, chromosome 19 at 44,524,553 bp
  • C to T, chromosome 19 at 46,897,625 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0565 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038756-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.