Strain Name:
C57BL/6J-MtgxR2233Btlr/Mmmh
Stock Number:
040234-MU
Citation ID:
RRID:MMRRC_040234-MU
Other Names:
R2233 (G1), C57BL/6J-MtgxR2233Btlr
Major Collection:

Strain Information

Zmpste24
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230709
Homologene: 4277
Chrm4
Name: cholinergic receptor, muscarinic 4
Synonyms: Chrm-4, muscarinic acetylcholine receptor 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12672
HGNC: HGNC:1953
Homologene: 20192
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Enah
Name: ENAH actin regulator
Synonyms: Mena, Ndpp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13800
Homologene: 134005
Mif4gd
Name: MIF4G domain containing
Synonyms: 1110014L05Rik, 2310075G12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69674
Homologene: 41389
Lrrc41
Name: leucine rich repeat containing 41
Synonyms: D730026A16Rik, MUF1, D630045E04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230654
Homologene: 4645
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Kifap3
Name: kinesin-associated protein 3
Synonyms: KAP3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16579
Homologene: 7799
Sf3a2
Name: splicing factor 3a, subunit 2
Synonyms: SFA66, PRP11, Sap62, 66kDa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20222
Homologene: 133823
Ints8
Name: integrator complex subunit 8
Synonyms: D130008D20Rik, 2810013E07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72656
Homologene: 9888
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: 1200015F06Rik, Mypt1, D10Ertd625e, 5730577I22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Rnf216
Name: ring finger protein 216
Synonyms: UIP83, F830018F18Rik, 2810055G22Rik, Ubce7ip1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108086
Homologene: 19442
Prss16
Name: serine protease 16 (thymus)
Synonyms: TSSP
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 54373
HGNC: HGNC:9480
Homologene: 38106
Crh
Name: corticotropin releasing hormone
Synonyms: CRF, corticotropin releasing factor, LOC383938, corticotropin-releasing factor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12918
HGNC: HGNC:2355
Homologene: 599
Txnl4b
Name: thioredoxin-like 4B
Synonyms: Dim2, D530025J19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234723
Homologene: 9880
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 4930400K19Rik, Tulip1, 2310003F20Rik, Garnl1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: nesprin-1, SYNE-1, enaptin165, A330049M09Rik, C130039F11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Dhx16
Name: DEAH-box helicase 16
Synonyms: 2410006N22Rik, DBP2, Ddx16, DEAH (Asp-Glu-Ala-His) box polypeptide 16
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 69192
HGNC: HGNC:2739
Homologene: 2658
Tmem135
Name: transmembrane protein 135
Synonyms: 2810439K08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72759
Homologene: 11295
Snap91
Name: synaptosomal-associated protein 91
Synonyms: F1-20, 91kDa, AP180
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20616
Homologene: 8429
Fezf1
Name: Fez family zinc finger 1
Synonyms: Zfp312-like, Fez, 3110069A13Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73191
Homologene: 19252
Grhl1
Name: grainyhead like transcription factor 1
Synonyms: p70 MGR, p61 MGR, LBP-32, Tcfcp2l2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 195733
VEGA: 12
Homologene: 32219
E2f4
Name: E2F transcription factor 4
Synonyms: 2010111M04Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 104394
HGNC: HGNC:3118
Homologene: 1471
Dync1i2
Name: dynein cytoplasmic 1 intermediate chain 2
Synonyms: 3110079H08Rik, Dncic2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13427
HGNC: HGNC:2964
Homologene: 37921
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23965
Homologene: 22673
Dazap1
Name: DAZ associated protein 1
Synonyms: 2410042M16Rik, mPrrp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70248
HGNC: HGNC:2683
Homologene: 134440
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: 1110014E10Rik, D530031C13Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320817
VEGA: 12
Homologene: 86351
Lmo2
Name: LIM domain only 2
Synonyms: Rbtn-2, Rbtn2, Rhom-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16909
HGNC: HGNC:6642
Homologene: 4072
Adarb1
Name: adenosine deaminase, RNA-specific, B1
Synonyms: RED1, ADAR2, D10Bwg0447e, 1700057H01Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110532
HGNC: HGNC:226
Homologene: 8280
Nphp3
Name: nephronophthisis 3 (adolescent)
Synonyms: D330020E01Rik, 3632410F03Rik, pcy, nephrocystin 3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74025
HGNC: HGNC:7907
Homologene: 32697
Nfkbid
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, delta
Synonyms: IkappaBNS
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243910
Homologene: 13101
Xylt2
Name: xylosyltransferase II
Synonyms: E030002B02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217119
Homologene: 23349
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, 9430076A06Rik, D430038H04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Ralgapa2
Name: Ral GTPase activating protein, alpha subunit 2 (catalytic)
Synonyms: A230067G21Rik, RGC2, AS250
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241694
Homologene: 28131
Sis
Name: sucrase isomaltase
Synonyms: Si-s, sucrase-isomaltase, 2010204N08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69983
Homologene: 37424
Kcnab1
Name: potassium voltage-gated channel, shaker-related subfamily, beta member 1
Synonyms: mKv(beta)1, Akr8a8, Kvbeta1.1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16497
HGNC: HGNC:6228
Homologene: 56491
Nynrin
Name: NYN domain and retroviral integrase containing
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 277154
VEGA: 14
Homologene: 19483
Gm12695
Name: predicted gene 12695
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 620779
Homologene: 51847
Cfap44
Name: cilia and flagella associated protein 44
Synonyms: 6330444M21Rik, D16Ertd642e, Wdr52
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 212517
Homologene: 75085
Scap
Name: SREBF chaperone
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235623
Homologene: 8160
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380698
Homologene: 70869
Rhobtb3
Name: Rho-related BTB domain containing 3
Synonyms: 1700040C17Rik, 4930503C18Rik, 2610033K01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 73296
Homologene: 8932
Stab1
Name: stabilin 1
Synonyms: MS-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 192187
Homologene: 9035
Or6z5
Name: olfactory receptor family 6 subfamily Z member 5
Synonyms: GA_x6K02T2QGBW-3203957-3204898, MOR103-6, Olfr1346
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258918
Homologene: 74134
Rabepk
Name: Rab9 effector protein with kelch motifs
Synonyms: 8430412M01Rik, 9530020D24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227746
Homologene: 48772
Gimap6
Name: GTPase, IMAP family member 6
Synonyms: 4833419H03Rik, Ian6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 231931
Homologene: 11670
Osbpl6
Name: oxysterol binding protein-like 6
Synonyms: ORP-6, 1110062M20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99031
Homologene: 101447
Slc25a29
Name: solute carrier family 25 (mitochondrial carrier, palmitoylcarnitine transporter), member 29
Synonyms: mCACL, CACL, C030003J19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 214663
Homologene: 5385
Tbx18
Name: T-box18
Synonyms: 2810012F10Rik, 2810404D13Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76365
Homologene: 11384
Lrrc49
Name: leucine rich repeat containing 49
Synonyms: D430025H09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102747
Homologene: 9782
Tnk1
Name: tyrosine kinase, non-receptor, 1
Synonyms: Kos1, Tnk1b, Tnk1a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 83813
Homologene: 2966
St3gal6
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Synonyms: ST3Gal VI, 1700023B24Rik, Siat10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 54613
Homologene: 4448
Iglon5
Name: IgLON family member 5
Synonyms: A230106M20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210094
Homologene: 46295
Scgb1b27
Name: secretoglobin, family 1B, member 27
Synonyms: Abp, Sal-1, Tcp, Abpa, Abpa27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11354
Homologene: 114479
Qrsl1
Name: glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1
Synonyms: GatA, 2700038P16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76563
Homologene: 6699
Vmn2r124
Name: vomeronasal 2, receptor 124
Synonyms: Gm7196, Vmn2r-ps113
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 637021
Homologene: 115024
Chrnb1
Name: cholinergic receptor nicotinic beta 1 subunit
Synonyms: Achr-2, Acrb, AChR beta
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11443
HGNC: HGNC:1961
Homologene: 594
Sel1l2
Name: sel-1 suppressor of lin-12-like 2 (C. elegans)
Synonyms: LOC228684
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228684
Homologene: 16802
Hyou1
Name: hypoxia up-regulated 1
Synonyms: CBP-140, Orp150, 140 kDa, Grp170, Cab140
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12282
Homologene: 4658
Or9m1
Name: olfactory receptor family 9 subfamily M member 1
Synonyms: GA_x6K02T2Q125-49403456-49402524, MOR173-2, Olfr1154
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258641
Homologene: 74192
Ctsm
Name: cathepsin M
Synonyms: Cat M, Catm, 1600027J17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 64139
VEGA: 13
Homologene: 75181
Ptpn18
Name: protein tyrosine phosphatase, non-receptor type 18
Synonyms: HSCF, FLP1, Ptpk1, PTP-K1, PTP-HSCF
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19253
HGNC: HGNC:9649
Homologene: 74971
Prl7a1
Name: prolactin family 7, subfamily a, member 1
Synonyms: PLP-G, PLP-E, Prlpg, Prlpe
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19113
Homologene: 136279
Gm11149
Name: predicted gene 11149
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100036537
VEGA: 9
Uck1
Name: uridine-cytidine kinase 1
Synonyms: URK1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22245
Homologene: 7990
Ccdc24
Name: coiled-coil domain containing 24
Synonyms: LOC381546
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381546
Zscan25
Name: zinc finger and SCAN domain containing 25
Synonyms: EG666311, Zfp498
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666311
Homologene: 64820
Sun3
Name: Sad1 and UNC84 domain containing 3
Synonyms: D630047F21Rik, Sunc1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 194974
Homologene: 51273
Otc
Name: ornithine transcarbamylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18416
HGNC: HGNC:8512
Homologene: 446
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,274,262 bp
  • G to T, chromosome 1 at 34,468,369 bp
  • A to G, chromosome 1 at 63,545,512 bp
  • T to A, chromosome 1 at 163,856,065 bp
  • G to A, chromosome 1 at 181,921,972 bp
  • T to C, chromosome 2 at 32,258,303 bp
  • A to T, chromosome 2 at 34,795,234 bp
  • C to T, chromosome 2 at 71,249,420 bp
  • T to C, chromosome 2 at 76,586,769 bp
  • G to A, chromosome 2 at 87,903,475 bp
  • A to G, chromosome 2 at 91,928,530 bp
  • T to C, chromosome 2 at 103,981,062 bp
  • T to C, chromosome 2 at 140,244,165 bp
  • A to G, chromosome 2 at 146,357,082 bp
  • A to T, chromosome 3 at 19,693,932 bp
  • T to A, chromosome 3 at 65,319,467 bp
  • A to T, chromosome 3 at 72,913,194 bp
  • G to A, chromosome 4 at 11,221,305 bp
  • C to T, chromosome 4 at 96,724,029 bp
  • G to A, chromosome 4 at 116,096,385 bp
  • T to C, chromosome 4 at 117,869,916 bp
  • T to C, chromosome 4 at 121,097,965 bp
  • G to A, chromosome 5 at 143,090,926 bp
  • A to G, chromosome 5 at 145,283,692 bp
  • T to C, chromosome 6 at 23,246,003 bp
  • T to A, chromosome 6 at 48,704,484 bp
  • T to C, chromosome 7 at 6,474,442 bp
  • T to A, chromosome 7 at 30,425,859 bp
  • T to C, chromosome 7 at 34,021,824 bp
  • T to C, chromosome 7 at 43,480,638 bp
  • T to A, chromosome 7 at 68,212,080 bp
  • T to C, chromosome 7 at 89,154,074 bp
  • T to C, chromosome 8 at 48,276,169 bp
  • T to A, chromosome 8 at 105,298,651 bp
  • A to G, chromosome 8 at 109,568,919 bp
  • G to A, chromosome 9 at 15,998,271 bp
  • C to T, chromosome 9 at 44,389,091 bp
  • A to G, chromosome 9 at 49,562,146 bp
  • A to T, chromosome 9 at 60,598,157 bp
  • T to C, chromosome 9 at 86,798,571 bp
  • G to T, chromosome 9 at 87,724,350 bp
  • G to A, chromosome 9 at 104,037,376 bp
  • T to C, chromosome 9 at 110,381,593 bp
  • T to C, chromosome 10 at 5,041,484 bp
  • T to C, chromosome 10 at 43,896,096 bp
  • A to G, chromosome 10 at 77,317,349 bp
  • A to G, chromosome 10 at 80,277,599 bp
  • G to T, chromosome 10 at 80,802,829 bp
  • A to G, chromosome 10 at 108,198,919 bp
  • T to A, chromosome 11 at 9,023,371 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • A to T, chromosome 11 at 69,795,602 bp
  • C to T, chromosome 11 at 69,855,191 bp
  • C to T, chromosome 11 at 94,669,996 bp
  • C to T, chromosome 11 at 115,609,525 bp
  • T to G, chromosome 12 at 5,006,745 bp
  • A to G, chromosome 12 at 24,608,511 bp
  • A to T, chromosome 12 at 55,717,071 bp
  • A to T, chromosome 12 at 108,835,661 bp
  • T to C, chromosome 13 at 22,009,409 bp
  • C to A, chromosome 13 at 27,642,419 bp
  • C to T, chromosome 13 at 61,538,901 bp
  • C to A, chromosome 13 at 75,872,365 bp
  • G to A, chromosome 14 at 31,161,880 bp
  • G to A, chromosome 14 at 55,872,067 bp
  • A to G, chromosome 16 at 44,451,525 bp
  • A to G, chromosome 16 at 58,473,534 bp
  • A to T, chromosome 17 at 18,049,665 bp
  • T to C, chromosome 17 at 35,887,886 bp
  • A to G, chromosome X at 10,303,367 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2233 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040234-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.