Strain Name:
C57BL/6J-MtgxR4270Btlr/Mmmh
Stock Number:
041075-MU
Citation ID:
RRID:MMRRC_041075-MU
Other Names:
R4270 (G1), C57BL/6J-MtgxR4270Btlr
Major Collection:

Strain Information

Cdh11
Name: cadherin 11
Synonyms: osteoblast-cadherin, OB-cadherin, Cad11
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12552
HGNC: HGNC:1750
Homologene: 1361
Ccdc88c
Name: coiled-coil domain containing 88C
Synonyms: Daple, 0610010D24Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68339
VEGA: 12
Homologene: 18903
Nbr1
Name: NBR1, autophagy cargo receptor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17966
HGNC: HGNC:6746
Homologene: 7438
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: chromatin remodeling factor, 8030453L17Rik, E430018P19Rik, KMT2H
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192195
Homologene: 10225
Slc30a5
Name: solute carrier family 30 (zinc transporter), member 5
Synonyms: Zntl1, ZnT-5, ZTL1, 1810010K08Rik, Znt5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69048
VEGA: 13
Homologene: 41503
Kif26a
Name: kinesin family member 26A
Synonyms: N-11 kinesin
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 668303
Homologene: 18970
Mxra8
Name: matrix-remodelling associated 8
Synonyms: 1200013A08Rik, Asp3, limitrin, DICAM
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74761
HGNC: HGNC:7542
Homologene: 11500
Ctsa
Name: cathepsin A
Synonyms: PPCA, Ppgb
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19025
HGNC: HGNC:9251
Homologene: 80163
Foxo1
Name: forkhead box O1
Synonyms: FKHR, Fkhr1, Afxh, Foxo1a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56458
HGNC: HGNC:3819
Homologene: 1527
Zfp597
Name: zinc finger protein 597
Synonyms: 4933407K12Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 71063
Homologene: 33641
Trim58
Name: tripartite motif-containing 58
Synonyms: LOC216781, LOC386443
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216781
Homologene: 9135
Nckap1l
Name: NCK associated protein 1 like
Synonyms: 4930568P13Rik, Hem1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105855
VEGA: 15
HGNC: HGNC:4862
Homologene: 3901
Trpc3
Name: transient receptor potential cation channel, subfamily C, member 3
Synonyms: Trp3, Trrp3, Trcp3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22065
Homologene: 20708
Fanca
Name: Fanconi anemia, complementation group A
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14087
HGNC: HGNC:3582
Homologene: 108
Xkr7
Name: X-linked Kx blood group related 7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228787
Homologene: 19390
Rwdd3
Name: RWD domain containing 3
Synonyms: 3110037C01Rik, 2510027J23Rik, RSUME
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66568
Homologene: 22899
Rimbp2
Name: RIMS binding protein 2
Synonyms: A930033C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231760
Homologene: 14672
Syt10
Name: synaptotagmin X
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 54526
Homologene: 10303
Nubp2
Name: nucleotide binding protein 2
Synonyms: D17Wsu11e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26426
VEGA: 17
HGNC: HGNC:8042
Homologene: 8057
Vmn2r107
Name: vomeronasal 2, receptor 107
Synonyms: V2r6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22312
Homologene: 129750
Or8c10
Name: olfactory receptor family 8 subfamily C member 10
Synonyms: GA_x6K02T2MYUG-19447-18473, MOR170-14, MOR170-8, GA_x6K02T2PVTD-32060891-32061865, Olfr899, Olfr250
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 404312
VEGA: 9
Homologene: 74151
Ctsh
Name: cathepsin H
Synonyms: Cat H
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13036
HGNC: HGNC:2535
Homologene: 36159
Aspg
Name: asparaginase
Synonyms: A530050D06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104816
VEGA: 12
Homologene: 113390
Rbms3
Name: RNA binding motif, single stranded interacting protein
Synonyms: 6720477E09Rik, 8430436O14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 207181
Homologene: 49388
Ighv1-43
Name: immunoglobulin heavy variable V1-43
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 629908
Gm17690
Name: predicted gene, 17690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Igkv9-120
Name: immunoglobulin kappa chain variable 9-120
Synonyms: Gm5571
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 434025
4930581F22Rik
Name: RIKEN cDNA 4930581F22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 78934
Pramel51
Name: PRAME like 51
Synonyms: Gm10436
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100039315
VEGA: 12
Homologene: 103830
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 153,054,315 bp
  • G to T, chromosome 2 at 164,835,302 bp
  • G to T, chromosome 3 at 36,662,925 bp
  • A to G, chromosome 3 at 52,345,405 bp
  • T to A, chromosome 3 at 88,982,040 bp
  • T to C, chromosome 3 at 96,167,891 bp
  • T to C, chromosome 3 at 121,158,901 bp
  • C to A, chromosome 4 at 155,841,137 bp
  • T to G, chromosome 5 at 128,819,777 bp
  • G to T, chromosome 6 at 68,050,367 bp
  • T to A, chromosome 8 at 102,664,626 bp
  • A to G, chromosome 8 at 123,268,794 bp
  • T to A, chromosome 9 at 35,126,349 bp
  • A to G, chromosome 9 at 38,367,701 bp
  • A to G, chromosome 9 at 90,061,598 bp
  • T to A, chromosome 9 at 117,056,748 bp
  • T to C, chromosome 11 at 58,651,267 bp
  • T to C, chromosome 11 at 101,567,222 bp
  • A to T, chromosome 12 at 88,178,283 bp
  • G to T, chromosome 12 at 100,947,219 bp
  • T to C, chromosome 12 at 112,121,195 bp
  • C to T, chromosome 12 at 112,173,414 bp
  • C to G, chromosome 12 at 114,946,152 bp
  • T to G, chromosome 13 at 100,829,013 bp
  • C to A, chromosome 15 at 89,790,892 bp
  • T to C, chromosome 15 at 103,473,122 bp
  • A to T, chromosome 16 at 3,872,090 bp
  • G to T, chromosome 17 at 20,355,779 bp
  • A to T, chromosome 17 at 24,885,593 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4270 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041075-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.