Strain Name:
C57BL/6J-MtgxR4440Btlr/Mmmh
Stock Number:
041705-MU
Citation ID:
RRID:MMRRC_041705-MU
Other Names:
R4440 (G1), C57BL/6J-MtgxR4440Btlr
Major Collection:

Strain Information

Ntrk2
Name: neurotrophic tyrosine kinase, receptor, type 2
Synonyms: trkB, C030027L06Rik, Tkrb
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18212
VEGA: 13
HGNC: HGNC:8032
Homologene: 4504
Mysm1
Name: myb-like, SWIRM and MPN domains 1
Synonyms: C530050H10Rik, C130067A03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320713
Homologene: 66204
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208628
Homologene: 32227
Hdac4
Name: histone deacetylase 4
Synonyms: 4932408F19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 208727
Homologene: 55946
Armc8
Name: armadillo repeat containing 8
Synonyms: HSPC056, 1200015K23Rik, Gid5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74125
Homologene: 9121
Nav2
Name: neuron navigator 2
Synonyms: POMFIL2, Unc53H2, HELAD1, RAINB2, 5330421F07Rik, Rainb1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78286
Homologene: 52330
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, Afadin, S-afadin, I-afadin, 5033403D15Rik, Mllt4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: mRPA1, RPA194, 194kDa, 3010014K16Rik, Rpo1-4, 2900087K15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20019
Homologene: 7033
Fip1l1
Name: factor interacting with PAPOLA and CPSF1
Synonyms: Rje, 1300019H17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66899
Homologene: 136254
Tbl1xr1
Name: transducin (beta)-like 1X-linked receptor 1
Synonyms: Ira1, TBLR1, DC42, C21, C230089I12Rik, A630076E03Rik, 8030499H02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 81004
Homologene: 69382
Adnp
Name: activity-dependent neuroprotective protein
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11538
Homologene: 7617
Tbr1
Name: T-box brain transcription factor 1
Synonyms: T-box brain gene 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21375
Homologene: 4807
C6
Name: complement component 6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12274
HGNC: HGNC:1339
Homologene: 47
Nipbl
Name: NIPBL cohesin loading factor
Synonyms: 4921518A06Rik, 4933421G18Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71175
VEGA: 15
Homologene: 15850
Mideas
Name: mitotic deacetylase associated SANT domain protein
Synonyms: 9430029N19Rik, C130039O16Rik, Elmsan1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238317
Homologene: 19330
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77505
Homologene: 131117
Klhl8
Name: kelch-like 8
Synonyms: 2310001P09Rik, D5Ertd431e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 246293
Homologene: 10819
Fastkd2
Name: FAST kinase domains 2
Synonyms: 2810421I24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75619
Homologene: 8957
Slc7a2
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 2
Synonyms: Tea, Atrc2, Cat2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11988
Homologene: 20659
Tespa1
Name: thymocyte expressed, positive selection associated 1
Synonyms: A430001F24Rik, 5830405N20Rik, Itprid3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67596
VEGA: 10
Homologene: 106645
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Pwwp2b
Name: PWWP domain containing 2B
Synonyms: D930023J19Rik, D7Ertd517e, Pwwp2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101631
Homologene: 19056
Angpt4
Name: angiopoietin 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11602
HGNC: HGNC:487
Homologene: 36302
Taok2
Name: TAO kinase 2
Synonyms: MAP3K17, TAO2, TAO1, PSK1, 1110033K02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381921
Homologene: 74531
Xab2
Name: XPA binding protein 2
Synonyms: 0610041O14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67439
Homologene: 5738
Vmn1r231
Name: vomeronasal 1 receptor 231
Synonyms: V1re7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171230
Atg2a
Name: autophagy related 2A
Synonyms: 1810013C15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 329015
Homologene: 86985
Lpin3
Name: lipin 3
Synonyms: 9130206L11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 64899
Homologene: 84844
Man2a2
Name: mannosidase 2, alpha 2
Synonyms: alpha mannosidase IIx, MX, 4931438M07Rik, 1700052O22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 140481
HGNC: HGNC:6825
Homologene: 55954
Slc14a2
Name: solute carrier family 14 (urea transporter), member 2
Synonyms: UT-A3, UT-A5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 27411
VEGA: 18
Homologene: 5183
Pramel15
Name: PRAME like 15
Synonyms: EG627009, Gm13125, Pramef20
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 627009
Homologene: 133194
Nkiras1
Name: NFKB inhibitor interacting Ras-like protein 1
Synonyms: KBRAS1, 2400004O09Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 69721
Homologene: 10670
Tex55
Name: testis expressed 55
Synonyms: 4930435E12Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74663
Homologene: 17614
Fpgs
Name: folylpolyglutamyl synthetase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14287
HGNC: HGNC:3824
Homologene: 6997
Or4c107
Name: olfactory receptor family 4 subfamily C member 107
Synonyms: GA_x6K02T2Q125-50437014-50437949, MOR233-20, MOR233-17, Olfr1212
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258241
Homologene: 66154
Ctnna3
Name: catenin alpha 3
Synonyms: Vr22, Catna3, 4930429L08Rik, alphaT-catenin, catenin (cadherin associated protein), alpha 3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216033
HGNC: HGNC:2511
Homologene: 56583
Ndufaf5
Name: NADH:ubiquinone oxidoreductase complex assembly factor 5
Synonyms: 2310003L22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69487
Homologene: 11474
Tle2
Name: transducin-like enhancer of split 2
Synonyms: Grg2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21886
Homologene: 20693
Cfc1
Name: cryptic, EGF-CFC family member 1
Synonyms: cryptic, b2b970Clo
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12627
Homologene: 50007
Alpl
Name: alkaline phosphatase, liver/bone/kidney
Synonyms: Akp-2, TNSALP, Akp2, TNAP, ALP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11647
HGNC: HGNC:438
Homologene: 37314
Or4a75
Name: olfactory receptor family 4 subfamily A member 75
Synonyms: GA_x6K02T2Q125-51059648-51058725, MOR231-10, Olfr1248
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258787
Homologene: 133613
Dpysl5
Name: dihydropyrimidinase-like 5
Synonyms: Crmp5, CRMP-5, CRAM
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 65254
Homologene: 41347
Rasgrf2
Name: RAS protein-specific guanine nucleotide-releasing factor 2
Synonyms: Grf2, 6330417G04Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19418
HGNC: HGNC:9876
Homologene: 2169
Bicdl2
Name: BICD family like cargo adaptor 2
Synonyms: Ccdc64b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 212733
VEGA: 17
Homologene: 17815
Smok3c
Name: sperm motility kinase 3C
Synonyms: EG622486
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 622486
Homologene: 128738
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 34,544,102 bp
  • G to A, chromosome 1 at 63,745,842 bp
  • C to A, chromosome 1 at 91,945,995 bp
  • C to T, chromosome 2 at 32,687,501 bp
  • A to T, chromosome 2 at 61,804,838 bp
  • A to G, chromosome 2 at 82,991,206 bp
  • G to A, chromosome 2 at 88,959,341 bp
  • G to T, chromosome 2 at 89,618,168 bp
  • T to A, chromosome 2 at 140,170,725 bp
  • G to T, chromosome 2 at 151,944,646 bp
  • A to G, chromosome 2 at 160,898,645 bp
  • G to T, chromosome 2 at 168,184,801 bp
  • A to G, chromosome 3 at 22,200,588 bp
  • T to C, chromosome 4 at 94,965,212 bp
  • A to T, chromosome 4 at 137,747,813 bp
  • A to T, chromosome 4 at 144,372,867 bp
  • T to C, chromosome 5 at 30,792,268 bp
  • T to C, chromosome 5 at 74,536,785 bp
  • A to G, chromosome 5 at 103,867,567 bp
  • T to C, chromosome 5 at 123,794,153 bp
  • T to A, chromosome 5 at 138,064,604 bp
  • A to G, chromosome 6 at 71,950,848 bp
  • C to T, chromosome 7 at 49,552,037 bp
  • A to G, chromosome 7 at 49,575,263 bp
  • C to A, chromosome 7 at 80,351,715 bp
  • G to A, chromosome 7 at 105,696,728 bp
  • C to T, chromosome 7 at 126,866,521 bp
  • T to C, chromosome 7 at 139,255,639 bp
  • T to C, chromosome 8 at 3,616,353 bp
  • T to C, chromosome 8 at 40,902,649 bp
  • T to A, chromosome 9 at 99,484,034 bp
  • A to G, chromosome 10 at 64,260,935 bp
  • A to G, chromosome 10 at 81,581,682 bp
  • C to T, chromosome 10 at 130,361,957 bp
  • C to T, chromosome 12 at 84,156,471 bp
  • G to C, chromosome 13 at 59,060,312 bp
  • T to C, chromosome 13 at 91,983,678 bp
  • C to T, chromosome 14 at 18,276,784 bp
  • A to T, chromosome 15 at 4,735,251 bp
  • G to C, chromosome 15 at 8,366,658 bp
  • T to C, chromosome 16 at 38,828,431 bp
  • T to C, chromosome 17 at 13,850,890 bp
  • T to C, chromosome 17 at 20,890,456 bp
  • C to T, chromosome 17 at 23,667,616 bp
  • A to G, chromosome 18 at 78,195,747 bp
  • T to C, chromosome 19 at 6,255,829 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4440 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041705-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.