Strain Name:
C57BL/6J-MtgxR4609Btlr/Mmmh
Stock Number:
041820-MU
Citation ID:
RRID:MMRRC_041820-MU
Other Names:
R4609 (G1), C57BL/6J-MtgxR4609Btlr
Major Collection:

Strain Information

Nap1l1
Name: nucleosome assembly protein 1-like 1
Synonyms: D10Ertd68e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53605
VEGA: 10
HGNC: HGNC:7637
Homologene: 129218
Clasp1
Name: CLIP associating protein 1
Synonyms: CLASP1, 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Zbtb43
Name: zinc finger and BTB domain containing 43
Synonyms: 1700010E06Rik, Zfp297b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71834
Homologene: 8514
Cilk1
Name: ciliogenesis associated kinase 1
Synonyms: 2210420N10Rik, Ick
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56542
Homologene: 69218
Morc3
Name: microrchidia 3
Synonyms: 1110051N18Rik, D16Jhu32e, 1110051N18Rik, Zcwcc3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 338467
Homologene: 32257
Trmt13
Name: tRNA methyltransferase 13
Synonyms: A930028L21Rik, 4631408H19Rik, Ccdc76
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229780
Homologene: 6875
Zfp462
Name: zinc finger protein 462
Synonyms: Gt4-2, 9430078C22Rik, Zfpip
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242466
Homologene: 41430
Septin11
Name: septin 11
Synonyms: 6230410I01Rik, D5Ertd606e, Sept11
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52398
Homologene: 56800
Palb2
Name: partner and localizer of BRCA2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233826
Homologene: 11652
Wdr3
Name: WD repeat domain 3
Synonyms: D030020G18Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269470
Homologene: 4937
Tanc2
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
Synonyms: 5730590C14Rik, 3526402J09Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77097
Homologene: 64680
Zbtb17
Name: zinc finger and BTB domain containing 17
Synonyms: mZ13, Miz1, Zfp100
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22642
Homologene: 2575
Ets2
Name: E26 avian leukemia oncogene 2, 3' domain
Synonyms: Ets-2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 23872
HGNC: HGNC:3489
Homologene: 3838
Hexa
Name: hexosaminidase A
Synonyms: Hex-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15211
VEGA: 9
HGNC: HGNC:4878
Homologene: 20146
Yipf1
Name: Yip1 domain family, member 1
Synonyms: C030002N13Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230584
Homologene: 5898
Dmxl2
Name: Dmx-like 2
Synonyms: E130119P06Rik, 6430411K14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235380
HGNC: HGNC:2938
Homologene: 41022
Tubb3
Name: tubulin, beta 3 class III
Synonyms: 3200002H15Rik, betaIII-tubulin, Tuj1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22152
Homologene: 68503
Trf
Name: transferrin
Synonyms: Tfn, HP
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22041
Homologene: 68153
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 627872
Homologene: 41287
Bmp1
Name: bone morphogenetic protein 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12153
VEGA: 14
HGNC: HGNC:1067
Homologene: 55955
Fhip1b
Name: FHF complex subunit HOOK interacting protein 1B
Synonyms: 4632419K20Rik, 6530415H11Rik, Fam160a2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74349
Homologene: 75329
Nfix
Name: nuclear factor I/X
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18032
HGNC: HGNC:7788
Homologene: 1872
Car9
Name: carbonic anhydrase 9
Synonyms: CAIX, MN/CA9
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230099
HGNC: HGNC:1383
Homologene: 20325
Cxcl16
Name: C-X-C motif chemokine ligand 16
Synonyms: SR-PSOX, 0910001K24Rik, SR-PSOX/CXCL16, Scavenger Receptor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66102
Homologene: 49694
Hk1
Name: hexokinase 1
Synonyms: Hk-1, mHk1-s, Hk1-s
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15275
HGNC: HGNC:4922
Homologene: 100530
Rb1
Name: RB transcriptional corepressor 1
Synonyms: pRb, Rb, Rb-1, retinoblastoma 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19645
HGNC: HGNC:9884
Homologene: 272
Ube2e3
Name: ubiquitin-conjugating enzyme E2E 3
Synonyms: ubcM2, Ubce4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22193
Homologene: 4636
Stard3nl
Name: STARD3 N-terminal like
Synonyms: 0610035N01Rik, 6530409L22Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76205
Homologene: 11501
Fem1c
Name: fem 1 homolog c
Synonyms: 3632443A22Rik, 2610312A07Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240263
Homologene: 10606
Dpy19l4
Name: dpy-19 like 4
Synonyms: LOC381510, Narg3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381510
Homologene: 18773
Pkd1l1
Name: polycystic kidney disease 1 like 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 171395
Homologene: 51376
Baiap3
Name: BAI1-associated protein 3
Synonyms: LOC381076
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 545192
HGNC: HGNC:948
Homologene: 20844
Wdr19
Name: WD repeat domain 19
Synonyms: C330027H04Rik, D330023L08Rik, Ift144, DYF2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 213081
Homologene: 11842
Nlgn2
Name: neuroligin 2
Synonyms: NL2, NLG2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216856
Homologene: 69317
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 672511
Homologene: 45439
Dpysl4
Name: dihydropyrimidinase-like 4
Synonyms: unc-33-like phosphoprotein 4, Ulip4, CRMP-3, Drp-4, DPY4, Crmp3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26757
HGNC: HGNC:3016
Homologene: 4691
Fbn2
Name: fibrillin 2
Synonyms: sy, Sne, Fib-2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Vmn2r88
Name: vomeronasal 2, receptor 88
Synonyms: V2r13, V2r3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 669149
Homologene: 129606
Bbs10
Name: Bardet-Biedl syndrome 10
Synonyms: 1300007O09Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71769
VEGA: 10
Homologene: 49781
Adam10
Name: a disintegrin and metallopeptidase domain 10
Synonyms: kuz, kuzbanian, 1700031C13Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11487
HGNC: HGNC:188
Homologene: 865
Nnt
Name: nicotinamide nucleotide transhydrogenase
Synonyms: 4930423F13Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18115
HGNC: HGNC:7863
Rhoj
Name: ras homolog family member J
Synonyms: TCL, 1110005O19Rik, TC10L, Arhj
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 80837
HGNC: HGNC:688
Homologene: 56894
Pus1
Name: pseudouridine synthase 1
Synonyms: MPUS1, mPus1p, A730013B20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56361
Homologene: 5931
Cntnap5b
Name: contactin associated protein-like 5B
Synonyms: Caspr5-2, C230078M14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241175
Homologene: 104295
Oasl2
Name: 2'-5' oligoadenylate synthetase-like 2
Synonyms: M1204, Mmu-OASL
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 23962
Homologene: 128369
Cadps2
Name: Ca2+-dependent activator protein for secretion 2
Synonyms: cpd2, A230044C21Rik, Caps2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320405
Homologene: 23060
Cpvl
Name: carboxypeptidase, vitellogenic-like
Synonyms: 4933436L16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71287
Homologene: 80235
Vps33b
Name: vacuolar protein sorting 33B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233405
Homologene: 10261
Ogg1
Name: 8-oxoguanine DNA-glycosylase 1
Synonyms: Mmh
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18294
HGNC: HGNC:8125
Homologene: 1909
Itih4
Name: inter alpha-trypsin inhibitor, heavy chain 4
Synonyms: Itih-4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16427
HGNC: HGNC:6169
Homologene: 1670
Or5p79
Name: olfactory receptor family 5 subfamily P member 79
Synonyms: GA_x6K02T2PBJ9-10951546-10952496, MOR204-7, Olfr507
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258738
Homologene: 27249
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Op1, Mater, Nalp5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23968
Homologene: 65105
Xirp1
Name: xin actin-binding repeat containing 1
Synonyms: Xin, mXin alpha, Cmya1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22437
VEGA: 9
Homologene: 7998
Chml
Name: choroideremia-like
Synonyms: Rep2, E030003F13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12663
HGNC: HGNC:1941
Homologene: 31055
Pde11a
Name: phosphodiesterase 11A
Synonyms: A630086N24Rik, 6330414F14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241489
HGNC: HGNC:8773
Homologene: 56763
Pygm
Name: muscle glycogen phosphorylase
Synonyms: PG
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19309
HGNC: HGNC:9726
Homologene: 2145
Skic8
Name: SKI8 subunit of superkiller complex
Synonyms: 2810418I05Rik, 2700038L12Rik, Wdr61
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66317
Homologene: 5884
Vmn1r233
Name: vomeronasal 1 receptor 233
Synonyms: V1rf5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171236
Homologene: 128343
Crocc2
Name: ciliary rootlet coiled-coil, rootletin family member 2
Synonyms: LOC381284, E030010N08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381284
Homologene: 141152
Setdb2
Name: SET domain, bifurcated 2
Synonyms: LOC239122, KMT1F
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239122
Homologene: 12903
Olfml2a
Name: olfactomedin-like 2A
Synonyms: 4932431K08Rik, photomedin-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241327
Homologene: 35248
Brdt
Name: bromodomain, testis-specific
Synonyms: 7420412D09Rik, Brd6, Fsrg3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 114642
HGNC: HGNC:1105
Homologene: 21064
Zbtb42
Name: zinc finger and BTB domain containing 42
Synonyms: simiRP58, Gm5188
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 382639
Homologene: 20003
Or4d5
Name: olfactory receptor family 4 subfamily D member 5
Synonyms: GA_x6K02T2PVTD-33799484-33798540, MOR239-6, Olfr984
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258601
VEGA: 9
Homologene: 17314
Maml3
Name: mastermind like transcriptional coactivator 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 433586
Homologene: 41284
Kcnq5
Name: potassium voltage-gated channel, subfamily Q, member 5
Synonyms: 9230107O05Rik, D1Mgi1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226922
HGNC: HGNC:6299
Homologene: 28270
Pcdhb20
Name: protocadherin beta 20
Synonyms: Pcdhb14, PcdhbT
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93891
HGNC: HGNC:8685
Homologene: 134303
Prr16
Name: proline rich 16
Synonyms: 5430406M13Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 71373
VEGA: 18
Homologene: 41152
Pou2af1
Name: POU domain, class 2, associating factor 1
Synonyms: OBF.1, BOB.1, OBF-1, Bob1, OCA-B, OCAB, Bob-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18985
VEGA: 9
HGNC: HGNC:9211
Homologene: 4543
Mief1
Name: mitochondrial elongation factor 1
Synonyms: Smcr7l
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239555
VEGA: 15
Homologene: 10374
AC153874.1
Name:
Type: Gene
Species: Mouse
Chromosome: 10
Nfkbie
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18037
VEGA: 17
HGNC: HGNC:7799
Homologene: 36160
Gm20528
Name: predicted gene 20528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Gm8919
Name: predicted gene 8919
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 667999
Dio3
Name: deiodinase, iodothyronine type III
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 107585
HGNC: HGNC:2885
Homologene: 1044
7530416G11Rik
Name: RIKEN cDNA 7530416G11 gene
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 328577
VEGA: 15
H2-Q5
Name: histocompatibility 2, Q region locus 5
Synonyms: H-2Q5, Qa-5, Qat-5, Qa5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15016
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 21,405,068 bp
  • A to C, chromosome 1 at 53,456,657 bp
  • A to T, chromosome 1 at 88,055,482 bp
  • G to A, chromosome 1 at 93,168,794 bp
  • G to A, chromosome 1 at 99,772,847 bp
  • A to G, chromosome 1 at 118,503,035 bp
  • A to T, chromosome 1 at 175,687,157 bp
  • A to T, chromosome 2 at 33,454,043 bp
  • G to T, chromosome 2 at 38,957,721 bp
  • T to C, chromosome 2 at 76,291,241 bp
  • A to G, chromosome 2 at 78,918,712 bp
  • T to C, chromosome 3 at 11,659,470 bp
  • G to T, chromosome 3 at 51,855,592 bp
  • C to T, chromosome 3 at 100,140,200 bp
  • T to C, chromosome 3 at 116,594,827 bp
  • A to T, chromosome 4 at 11,295,999 bp
  • A to T, chromosome 4 at 43,507,267 bp
  • C to T, chromosome 4 at 55,011,889 bp
  • G to A, chromosome 4 at 107,344,683 bp
  • A to G, chromosome 4 at 141,466,498 bp
  • A to G, chromosome 5 at 65,228,542 bp
  • G to T, chromosome 5 at 93,162,254 bp
  • G to A, chromosome 5 at 107,359,936 bp
  • A to G, chromosome 5 at 110,780,318 bp
  • A to T, chromosome 5 at 114,899,796 bp
  • A to T, chromosome 6 at 23,587,579 bp
  • A to T, chromosome 6 at 53,974,620 bp
  • A to C, chromosome 6 at 113,328,432 bp
  • A to T, chromosome 7 at 23,417,748 bp
  • A to G, chromosome 7 at 80,291,118 bp
  • A to G, chromosome 7 at 105,388,224 bp
  • A to T, chromosome 7 at 108,622,504 bp
  • G to T, chromosome 7 at 122,124,723 bp
  • T to C, chromosome 7 at 139,098,621 bp
  • A to T, chromosome 8 at 84,726,490 bp
  • A to G, chromosome 8 at 123,420,919 bp
  • T to C, chromosome 9 at 40,100,806 bp
  • G to A, chromosome 9 at 51,238,225 bp
  • A to G, chromosome 9 at 54,446,512 bp
  • A to G, chromosome 9 at 54,728,179 bp
  • T to C, chromosome 9 at 59,557,319 bp
  • T to C, chromosome 9 at 70,740,143 bp
  • T to A, chromosome 9 at 78,167,789 bp
  • C to T, chromosome 9 at 103,211,985 bp
  • T to C, chromosome 9 at 120,016,506 bp
  • G to T, chromosome 10 at 62,358,415 bp
  • A to T, chromosome 10 at 77,826,796 bp
  • A to G, chromosome 10 at 111,301,134 bp
  • T to A, chromosome 10 at 111,492,880 bp
  • C to T, chromosome 11 at 8,958,964 bp
  • A to T, chromosome 11 at 69,834,086 bp
  • T to C, chromosome 11 at 70,455,429 bp
  • A to G, chromosome 11 at 105,910,240 bp
  • A to G, chromosome 11 at 119,437,695 bp
  • A to T, chromosome 12 at 75,400,206 bp
  • G to T, chromosome 12 at 110,280,010 bp
  • C to T, chromosome 12 at 112,680,542 bp
  • G to T, chromosome 13 at 19,370,264 bp
  • T to C, chromosome 13 at 119,357,536 bp
  • G to A, chromosome 14 at 30,901,669 bp
  • A to T, chromosome 14 at 51,418,074 bp
  • T to C, chromosome 14 at 59,415,704 bp
  • C to T, chromosome 14 at 70,477,966 bp
  • T to A, chromosome 14 at 73,262,514 bp
  • C to A, chromosome 15 at 80,248,253 bp
  • T to C, chromosome 15 at 85,494,169 bp
  • A to G, chromosome 16 at 93,864,968 bp
  • A to T, chromosome 16 at 95,711,774 bp
  • A to T, chromosome 17 at 20,994,415 bp
  • A to T, chromosome 17 at 25,250,261 bp
  • A to T, chromosome 17 at 35,397,080 bp
  • A to T, chromosome 17 at 45,558,584 bp
  • G to A, chromosome 18 at 37,505,796 bp
  • A to T, chromosome 18 at 46,505,948 bp
  • A to G, chromosome 18 at 51,118,067 bp
  • A to T, chromosome 18 at 58,190,269 bp
  • T to A, chromosome 19 at 6,391,409 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4609 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041820-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.