Strain Name:
B6;129P2-Ptentm1Mak/Mmjax
Stock Number:
042059-JAX
Citation ID:
RRID:MMRRC_042059-JAX
Other Names:
PTEN, MMAC, TEP1

Strain Information

Ptentm1Mak
Name: phosphatase and tensin homolog; targeted mutation 1, Tak W Mak
Synonyms: mPTEN3-5, Pten-, mPTEN-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
Pten
Name: phosphatase and tensin homolog
Synonyms: MMAC1, TEP1, 2310035O07Rik, A130070J02Rik, B430203M17Rik
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Knockout
NCBI: 19211
HGNC: HGNC:9588
Homologene: 265
Genetic Alterations
Exons 3 to 5 of the Pten gene (phosphatase and tensin homolog) are deleted in this mouse. Knock-out mice exhibit an embryonic lethal phenotype. Heterozygous mPTEN3-5 mice develop T cell lymphomas associated with loss of heterozygosity of the wild-type allele. These mice may be useful for studies of cancer progression.

Genotype Determination
ES Cell Line
E14K
Phenotype
The Pten (phosphatase and tensin homolog) gene is a dual specificity protein phosphatase and encodes a tumor suppressor that functions through negative regulation of the AKT/PKB signaling pathway. Mutations in PTEN are associated with multiple primary malignant tumors. Embryos homozygous for the knock-out allele have a lethal phenotype; exhibiting abnormal patterning in cephalic and caudal regions and defective placentation at E8.5. Death occurs by E9.5 due to defective chorio-allantoic development. Mice heterozygous for the mPTEN3-5 allele have a high incidence of T cell lymphomas associated with loss of heterozygosity of the wild-type allele. Tumor formation is accelerated by gamma-irradiation and is associated with elevated phosphorylation of AKT/PKB. These mice may be useful for studies of cancer progression.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Embryonic and Fetal Development/genetics
  • Exons
  • Female
  • Fetal Death/genetics
  • Gamma Rays
  • Genes, Tumor Suppressor
  • Genetic Predisposition to Disease/genetics
  • Genotype
  • Lymphoma, T-Cell/genetics
  • Mice
  • Mice, Mutant Strains
  • Neoplasms/genetics
  • PTEN Phosphohydrolase
  • Phenotype
  • Phosphoric Monoester Hydrolases/genetics
  • Polymerase Chain Reaction
  • Pregnancy
  • Proto-Oncogenes
  • Recombination, Genetic
  • Sequence Deletion
  • Tumor Suppressor Proteins
Strain Development
A targeting vector containing a PGK-neomycin cassette, in reverse orientation, was used to replace the splice donor site of exon 2 and exons 3, 4 and 5. Exon 5 encodes a phosphatase domain. The construct was electroporated into 129P2/OlaHsd derived E14K embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were bred to C57BL/6J to achieve germline transmission. The mice were then backcrossed to C57BL/6 for at least 9 generations (see SNP note below). Upon arrival, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm is used to fertilize C57BL/6J.

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Six of the 43 markers throughout the genome are segregating, suggesting an incomplete backcross. Also, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory MMRRC were on a mixed C57BL/6J ; C57BL/6N genetic background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Developmental Biology
Donor
Tak Mak, Ph.D., Campbell Family Institute for Breast Cancer Research.
Primary Reference

Suzuki A, de la Pompa JL, Stambolic V, Elia AJ, Sasaki T, del Barco Barrantes I, Ho A, Wakeham A, Itie A, Khoo W, Fukumoto M, Mak TW. High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice. Curr Biol. 1998 Oct 22;8(21):1169-78. (Medline PMID: 9799734)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Other
N/A
MMRRC Breeding System
Backcross
Generation
N9+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042059-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
042059-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.