Strain Detail Sheet

Strain Name:
B6;129-Rev3ltm2.1Rwd Polhtm1Pjg/Mmucd
Stock Number:
042063-UCD
Citation ID:
RRID:MMRRC_042063-UCD
Other Names:
Pol zeta+/-Pol eta-/-,

Strain Information

Gene Details

Rev3ltm2.1Rwd
Name: REV3 like, DNA directed polymerase zeta catalytic subunit; targeted mutation 2.1, Richard D Wood
Synonyms: Rev3LloxP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Conditional
Polhtm1Pjg
Name:
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Conditional
Rev3l
Name: REV3 like, DNA directed polymerase zeta catalytic subunit
Synonyms: Sez4, Rev
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Conditional
NCBI: 19714
HGNC: HGNC:9968
Homologene: 48147
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Polh
Name: polymerase (DNA directed), eta (RAD 30 related)
Synonyms: RAD30A
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Conditional
NCBI: 80905
VEGA: 17
HGNC: HGNC:9181
Homologene: 38189
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
see citation
Genotype Determination
ES Cell Line
Not specified

Strain Description

Phenotype

Homozygous: < A:T mutations

Heterozygous: < A:T mutations

Cre-excised Phenotype: Undetermined

Strain Development
Pol ζ(+/-) mice on a mixed C57BL/6 X 129 background were obtained from J. Wittschieben and R. Wood (University of Texas, MD Anderson Cancer Center, Smithville, TX; PMCID:PMC3008348) and bred to Pol η(-/-) mice on a C57BL/6 background (PMID:15939880). We have previously reported that SHM is identical in both the C57BL/6 and 129 strains of mice (PMID:12925679). Genotypes were confirmed by PCR of tail DNA using Rev3L+ and Rev3L- primer sets (PMCID:PMC3008348) and Polζ primer sets (PMID:15939880). Pol ζ(+/-)/Pol η(-/-) mice were then bred to each other, which generated Pol ζ(+/+) Pol η (-/-) mice and Pol ζ (+/-) Pol η (-/-) mice. Littermate mice were used at 3–8 mo of age.

The MMRRC center will use C57BL/6J oocyte donors for generation of embryos for cryopreservation and cryo-recovery procedures
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor
Patricia Gearhart, Ph.D., National Institute on Aging.

Colony and Husbandry Information

Special Considerations

n/a

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
albino

Breeding

MMRRC Breeding System
Sib-mating
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months Greater than 12 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.