Strain Name:
C57BL/6J-MtgxR5195Btlr/Mmmh
Stock Number:
042771-MU
Citation ID:
RRID:MMRRC_042771-MU
Other Names:
R5195 (G1), C57BL/6J-MtgxR5195Btlr
Major Collection:

Strain Information

Kcns2
Name: K+ voltage-gated channel, subfamily S, 2
Synonyms: Kv9.2, E130006J24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16539
VEGA: 15
HGNC: HGNC:6301
Homologene: 22465
Gtf2i
Name: general transcription factor II I
Synonyms: TFII-I, BAP-135, 6030441I21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14886
HGNC: HGNC:4659
Homologene: 7748
Senp6
Name: SUMO/sentrin specific peptidase 6
Synonyms: E130319N12Rik, 2810017C20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 215351
Homologene: 9196
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Sik3
Name: SIK family kinase 3
Synonyms: 5730525O22Rik, 9030204A07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70661
Homologene: 57023
Snrnp70
Name: small nuclear ribonucleoprotein 70 (U1)
Synonyms: Rnulp70, U1-70, 3200002N22Rik, 2700022N21Rik, Srnp70, Snrp70
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20637
Homologene: 20672
Cobl
Name: cordon-bleu WH2 repeat
Synonyms: C530045F18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12808
Homologene: 9058
Hmgn2
Name: high mobility group nucleosomal binding domain 2
Synonyms: Hmg17, HMG-17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15331
HGNC: HGNC:4986
Homologene: 128537
Lars2
Name: leucyl-tRNA synthetase, mitochondrial
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102436
VEGA: 9
Homologene: 6526
St7
Name: suppression of tumorigenicity 7
Synonyms: RAY1, HELG, SEN4, TSG7, Fam4a2, 9430001H04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 64213
Homologene: 10185
Taf13
Name: TATA-box binding protein associated factor 13
Synonyms: 2010309N11Rik, 1810004N01Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99730
Homologene: 4126
Slc41a3
Name: solute carrier family 41, member 3
Synonyms: SLC41A1-L2, 1010001P06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71699
Homologene: 23052
Cpt1a
Name: carnitine palmitoyltransferase 1a, liver
Synonyms: CPTI, L-CPT I, Cpt1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12894
VEGA: 19
HGNC: HGNC:2328
Homologene: 1413
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 4833421P10Rik, A130030D10Rik, 1110059H15Rik, Zfp650
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68795
Homologene: 52092
Cep120
Name: centrosomal protein 120
Synonyms: Ccdc100
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225523
VEGA: 18
Homologene: 27415
Inpp5j
Name: inositol polyphosphate 5-phosphatase J
Synonyms: Pipp, Pib5pa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 170835
HGNC: HGNC:8956
Homologene: 8682
Tnc
Name: tenascin C
Synonyms: Hxb, TN-C, TN, C130033P17Rik, tenascin-C, cytotactin, hexabrachion
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21923
HGNC: HGNC:5318
Homologene: 55636
Lgr6
Name: leucine-rich repeat-containing G protein-coupled receptor 6
Synonyms: A530037C04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329252
Homologene: 49680
Muc20
Name: mucin 20
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224116
Homologene: 17135
Crk
Name: v-crk avian sarcoma virus CT10 oncogene homolog
Synonyms: proto-oncogene c-crk, Crk-II, Crk-I, Crko, Crk-III
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12928
HGNC: HGNC:2362
Homologene: 81850
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Pkd2
Name: polycystin 2, transient receptor potential cation channel
Synonyms: polycystin-2, C030034P18Rik, TRPP2, PC2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18764
HGNC: HGNC:9009
Homologene: 20104
Pgam5
Name: phosphoglycerate mutase family member 5
Synonyms: 2610528A17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72542
Homologene: 14597
Bicra
Name: BRD4 interacting chromatin remodeling complex associated protein
Synonyms: Gltscr1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243842
HGNC: HGNC:4332
Homologene: 9250
Pde4a
Name: phosphodiesterase 4A, cAMP specific
Synonyms: dunce, Dpde2, D9Ertd60e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18577
HGNC: HGNC:8780
Homologene: 4520
Zbed5
Name: zinc finger BED-type containing 5
Synonyms: 2410018M08Rik, Zbed5, Chchd2l
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71970
Homologene: 84838
Maml2
Name: mastermind like transcriptional coactivator 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270118
Homologene: 134147
Wdr89
Name: WD repeat domain 89
Synonyms: 2600001A11Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 72338
Homologene: 43791
Epha3
Name: Eph receptor A3
Synonyms: Tyro4, Mek4, Hek4, Hek, Cek4, End3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13837
VEGA: 16
HGNC: HGNC:3387
Homologene: 21083
Myo3a
Name: myosin IIIA
Synonyms: 9030416P08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 667663
HGNC: HGNC:7601
Homologene: 49486
Trpm1
Name: transient receptor potential cation channel, subfamily M, member 1
Synonyms: Mlsn1, 4732499L03Rik, melastatin, LTRPC1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17364
HGNC: HGNC:7146
Homologene: 19940
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380698
Homologene: 70869
Deup1
Name: deuterosome assembly protein 1
Synonyms: 4933401K09Rik, Ccdc67
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 234964
Homologene: 18760
Krt86
Name: keratin 86
Synonyms: MHb4, Krt2-11, Khb4, Krt2-10
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16679
VEGA: 15
HGNC: HGNC:6463
Homologene: 1717
Stab1
Name: stabilin 1
Synonyms: MS-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 192187
Homologene: 9035
Rbbp8
Name: retinoblastoma binding protein 8, endonuclease
Synonyms: 9930104E21Rik, CtIP
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225182
VEGA: 18
HGNC: HGNC:9891
Homologene: 28546
Or10d5
Name: olfactory receptor family 10 subfamily D member 5
Synonyms: GA_x6K02T2PVTD-33651220-33650288, MOR224-2, Olfr975
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258825
VEGA: 9
Homologene: 27275
Slc22a30
Name: solute carrier family 22, member 30
Synonyms: C730048C13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 319800
Homologene: 77136
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 435626
Homologene: 52359
Lhcgr
Name: luteinizing hormone/choriogonadotropin receptor
Synonyms: Lhr, Gpcr19-rs1, LH-R
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16867
VEGA: 17
HGNC: HGNC:6585
Homologene: 37276
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Fanca
Name: Fanconi anemia, complementation group A
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14087
HGNC: HGNC:3582
Homologene: 108
Or11g2
Name: olfactory receptor family 11 subfamily G member 2
Synonyms: GA_x6K02T2PMLR-6326342-6327313, MOR106-13P, Olfr744
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 257884
Homologene: 128063
Mylk
Name: myosin, light polypeptide kinase
Synonyms: telokin, Mlck, MLCK210, MLCK108, 9530072E15Rik, A930019C19Rik, nmMlck
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 107589
HGNC: HGNC:7590
Homologene: 14202
Gbp4
Name: guanylate binding protein 4
Synonyms: Mag-2, Mpa-2, Mpa2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17472
Homologene: 128731
Arhgef40
Name: Rho guanine nucleotide exchange factor 40
Synonyms: E130112L23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268739
Homologene: 19560
Ccdc78
Name: coiled-coil domain containing 78
Synonyms: LOC381146, LOC381077
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381077
VEGA: 17
Homologene: 105408
Kbtbd3
Name: kelch repeat and BTB (POZ) domain containing 3
Synonyms: 2200003A07Rik, Bklhd3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69149
Homologene: 12300
Barhl2
Name: BarH like homeobox 2
Synonyms: MBH1, E130309B19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 104382
HGNC: HGNC:954
Homologene: 10572
Kptn
Name: kaptin
Synonyms: 2E4, actin-binding protein, 2310042D10Rik, C030013F01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70394
HGNC: HGNC:6404
Homologene: 5127
Prr14
Name: proline rich 14
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233895
Homologene: 11424
Apoo-ps
Name: apolipoprotein O, pseudogene
Synonyms: EG621156, Apoo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 621156
VEGA: 13
Hook2
Name: hook microtubule tethering protein 2
Synonyms: A630054I03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 170833
Homologene: 8332
Klhl31
Name: kelch-like 31
Synonyms: D930047P17Rik, Kbtbd1, 9830147P19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244923
Homologene: 27819
Tmem200a
Name: transmembrane protein 200A
Synonyms: C030003D03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77220
VEGA: 10
Homologene: 14175
Toe1
Name: target of EGR1, member 1 (nuclear)
Synonyms: 4933424D16Rik, 4930584N22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68276
Homologene: 11823
Zeb2
Name: zinc finger E-box binding homeobox 2
Synonyms: SIP1, Zfx1b, 9130203F04Rik, D130016B08Rik, Zfhx1b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24136
Homologene: 8868
Insyn2a
Name: inhibitory synaptic factor 2A
Synonyms: B830028B13Rik, Fam196a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 627214
Homologene: 85469
Mrps9
Name: mitochondrial ribosomal protein S9
Synonyms: 2310002A08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 69527
Homologene: 32565
Ryk
Name: receptor-like tyrosine kinase
Synonyms: Vik, ERK-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20187
Homologene: 68287
Pcsk1
Name: proprotein convertase subtilisin/kexin type 1
Synonyms: PC3, prohormone convertase 1/3, Nec-1, Nec1, PC1, SPC3, Phpp-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18548
HGNC: HGNC:8743
Homologene: 379
Med24
Name: mediator complex subunit 24
Synonyms: Trap100, D11Ertd307e, R75526, 100kDa, DRIP100, Gse2, Pparb2, Thrap4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 23989
Homologene: 40795
Efcab15
Name: EF-hand calcium binding domain 15
Synonyms: 1700023F06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69441
Homologene: 132455
Slc35e4
Name: solute carrier family 35, member E4
Synonyms: A330108F03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103710
Homologene: 37409
Malrd1
Name: MAM and LDL receptor class A domain containing 1
Synonyms: Gm13318, Gm13364, Diet1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 102635496
Homologene: 136214
Spag17os
Name: sperm associated antigen 17, opposite strand
Synonyms: B930086A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Ccnb1-ps
Name: cyclin B1, pseudogene
Synonyms: Gm5593
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434175
HGNC: HGNC:1579
Homologene: 68982
Igkv13-54-1
Name: immunoglobulin kappa chain variable 13-54-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 692105
RP23-201D22.4
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Snora15
Name: small nucleolar RNA, H/ACA box 15
Synonyms: MBI-79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100113364
VEGA: 5
Igkv19-93
Name: immunoglobulin kappa chain variable 19-93
Synonyms: Vx38C, Igk-V38
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 692161
RP24-187P11.4
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 42,898,574 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • C to T, chromosome 2 at 16,150,810 bp
  • T to A, chromosome 2 at 22,291,889 bp
  • T to C, chromosome 2 at 45,001,635 bp
  • G to A, chromosome 2 at 69,956,034 bp
  • T to C, chromosome 3 at 100,101,388 bp
  • G to A, chromosome 3 at 108,581,074 bp
  • A to T, chromosome 4 at 63,967,252 bp
  • T to C, chromosome 4 at 116,804,655 bp
  • C to A, chromosome 4 at 133,967,286 bp
  • T to A, chromosome 4 at 143,950,880 bp
  • T to A, chromosome 4 at 144,271,741 bp
  • T to C, chromosome 5 at 34,306,969 bp
  • G to A, chromosome 5 at 104,486,681 bp
  • T to A, chromosome 5 at 105,119,532 bp
  • A to G, chromosome 5 at 106,453,439 bp
  • A to T, chromosome 5 at 110,265,988 bp
  • A to T, chromosome 5 at 129,794,655 bp
  • G to T, chromosome 5 at 129,902,178 bp
  • A to T, chromosome 5 at 134,244,832 bp
  • A to G, chromosome 6 at 17,743,637 bp
  • T to A, chromosome 6 at 68,736,526 bp
  • A to T, chromosome 6 at 69,617,421 bp
  • T to C, chromosome 6 at 90,633,671 bp
  • G to A, chromosome 7 at 15,979,953 bp
  • A to G, chromosome 7 at 16,123,103 bp
  • T to A, chromosome 7 at 42,106,098 bp
  • T to A, chromosome 7 at 45,394,710 bp
  • T to C, chromosome 7 at 64,237,693 bp
  • A to T, chromosome 7 at 127,474,577 bp
  • A to T, chromosome 7 at 134,884,416 bp
  • A to T, chromosome 8 at 84,994,776 bp
  • A to G, chromosome 8 at 123,303,945 bp
  • A to T, chromosome 8 at 125,801,549 bp
  • G to C, chromosome 9 at 4,316,905 bp
  • T to A, chromosome 9 at 13,621,114 bp
  • A to T, chromosome 9 at 15,575,191 bp
  • A to G, chromosome 9 at 21,204,333 bp
  • A to G, chromosome 9 at 39,950,679 bp
  • G to T, chromosome 9 at 46,208,844 bp
  • A to G, chromosome 9 at 77,650,290 bp
  • T to C, chromosome 9 at 80,140,823 bp
  • T to C, chromosome 9 at 102,867,613 bp
  • T to A, chromosome 9 at 109,525,966 bp
  • T to C, chromosome 9 at 123,453,310 bp
  • T to C, chromosome 10 at 26,078,956 bp
  • A to C, chromosome 11 at 3,499,889 bp
  • T to A, chromosome 11 at 3,912,872 bp
  • C to T, chromosome 11 at 12,253,565 bp
  • C to T, chromosome 11 at 59,060,850 bp
  • T to C, chromosome 11 at 69,744,079 bp
  • T to A, chromosome 11 at 75,679,463 bp
  • T to C, chromosome 11 at 98,710,281 bp
  • T to C, chromosome 11 at 103,198,968 bp
  • C to T, chromosome 12 at 75,633,288 bp
  • T to C, chromosome 13 at 75,126,855 bp
  • T to C, chromosome 13 at 107,414,553 bp
  • C to A, chromosome 14 at 31,140,521 bp
  • T to C, chromosome 14 at 50,618,786 bp
  • T to A, chromosome 14 at 51,989,812 bp
  • G to A, chromosome 15 at 34,839,531 bp
  • T to A, chromosome 15 at 101,476,933 bp
  • G to A, chromosome 16 at 32,794,476 bp
  • T to A, chromosome 16 at 34,979,215 bp
  • C to T, chromosome 16 at 63,546,147 bp
  • T to A, chromosome 17 at 25,789,988 bp
  • A to G, chromosome 17 at 67,764,800 bp
  • A to T, chromosome 17 at 88,742,946 bp
  • T to C, chromosome 18 at 11,722,151 bp
  • T to A, chromosome 18 at 53,721,698 bp
  • A to T, chromosome 19 at 3,383,800 bp
  • G to A, chromosome 19 at 8,344,393 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5195 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042771-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.