Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6086Btlr/Mmmh
Stock Number:
044427-MU
Citation ID:
RRID:MMRRC_044427-MU
Other Names:
R6086 (G1)
Major Collection:

Strain Information

Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, semaV, sema5, SemA, LUCA-1, Semaa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20347
Homologene: 20972
Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Crlf3
Name: cytokine receptor-like factor 3
Synonyms: cytor4, Creme9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54394
Homologene: 9327
Cdyl2
Name: chromodomain protein, Y chromosome-like 2
Synonyms: 1700029M19Rik, 4930453I21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 75796
Homologene: 41779
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, Npps2, PD-Ialpha, Pdnp2, ATX
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Zranb2
Name: zinc finger, RAN-binding domain containing 2
Synonyms: Znf265, Zis, Zfp265
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 53861
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 75,473,243 bp
  • T to C, chromosome 1 at 92,917,681 bp
  • C to T, chromosome 1 at 160,086,120 bp
  • C to A, chromosome 1 at 162,321,033 bp
  • A to G, chromosome 2 at 89,296,854 bp
  • AAGT to AAGTAGT, chromosome 2 at 98,663,803 bp
  • A to G, chromosome 2 at 119,094,068 bp
  • A to G, chromosome 2 at 132,538,114 bp
  • T to C, chromosome 3 at 95,130,285 bp
  • A to G, chromosome 3 at 97,205,524 bp
  • T to C, chromosome 3 at 157,543,246 bp
  • C to T, chromosome 4 at 101,597,807 bp
  • T to C, chromosome 4 at 106,613,206 bp
  • T to C, chromosome 5 at 45,693,236 bp
  • A to T, chromosome 5 at 70,754,053 bp
  • A to C, chromosome 5 at 72,541,019 bp
  • T to A, chromosome 5 at 77,061,305 bp
  • C to T, chromosome 5 at 100,036,481 bp
  • T to A, chromosome 5 at 143,563,041 bp
  • A to G, chromosome 6 at 34,874,620 bp
  • A to G, chromosome 6 at 115,560,075 bp
  • G to T, chromosome 6 at 115,733,667 bp
  • A to G, chromosome 6 at 116,256,216 bp
  • T to A, chromosome 6 at 124,039,560 bp
  • G to C, chromosome 6 at 124,191,999 bp
  • T to A, chromosome 7 at 44,201,734 bp
  • G to A, chromosome 7 at 74,318,590 bp
  • G to T, chromosome 7 at 80,395,431 bp
  • A to T, chromosome 7 at 84,588,912 bp
  • A to G, chromosome 7 at 140,836,798 bp
  • T to G, chromosome 8 at 93,027,353 bp
  • A to G, chromosome 8 at 116,589,296 bp
  • A to G, chromosome 8 at 122,501,657 bp
  • T to C, chromosome 9 at 48,947,298 bp
  • C to T, chromosome 9 at 59,790,057 bp
  • T to C, chromosome 9 at 75,500,123 bp
  • T to C, chromosome 9 at 107,600,848 bp
  • T to A, chromosome 11 at 36,008,646 bp
  • C to T, chromosome 11 at 65,989,915 bp
  • A to C, chromosome 11 at 66,085,174 bp
  • T to C, chromosome 11 at 69,516,008 bp
  • G to A, chromosome 11 at 79,683,480 bp
  • C to T, chromosome 11 at 80,048,610 bp
  • G to T, chromosome 12 at 8,015,164 bp
  • G to A, chromosome 12 at 29,832,332 bp
  • T to C, chromosome 13 at 8,030,033 bp
  • T to C, chromosome 13 at 19,263,284 bp
  • C to A, chromosome 13 at 46,635,712 bp
  • T to A, chromosome 13 at 47,035,030 bp
  • T to A, chromosome 13 at 49,287,296 bp
  • C to T, chromosome 13 at 51,713,745 bp
  • A to T, chromosome 14 at 65,815,253 bp
  • A to T, chromosome 14 at 99,062,294 bp
  • G to T, chromosome 15 at 38,296,937 bp
  • T to C, chromosome 15 at 54,845,834 bp
  • C to A, chromosome 15 at 58,150,628 bp
  • G to A, chromosome 15 at 59,654,475 bp
  • A to G, chromosome 15 at 65,889,711 bp
  • T to C, chromosome 15 at 76,704,587 bp
  • T to A, chromosome 15 at 80,257,453 bp
  • T to A, chromosome 16 at 95,980,044 bp
  • T to A, chromosome 17 at 33,302,504 bp
  • T to C, chromosome 17 at 34,978,155 bp
  • A to C, chromosome 18 at 34,357,131 bp
  • A to G, chromosome 19 at 13,102,381 bp
  • C to G, chromosome X at 9,450,750 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6086 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044427-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.