Strain Name: B6.129S2-Dntttm1Gfn/Mmucd
Stock Number: 000045-UCD
Other Names:
- TdT-/-, TdT0 , B6.129S2-Dntttm1Gfn/Mmcd
Gene Details: (provided by MGI)
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Allele Symbol: Dntttm1Gfn Name: targeted mutation 1, Susan Gilfillan Chromosome: 19 Alteration at locus: Knockout |
Genetic Alterations:
A neo cassette was inserted into exon 4.
Genotype Determination:
ES Cell Line: D3
Strain Description
Phenotype
Dntt-/- mice don't express terminal deoxynucleotidyl transferase and therefore lack "N" nucleotide insertions at the junctions of Immunoglobulin (Ig) and T cell receptor (TCR) V(D)J rearrangements; consequently their Ig and TCR repertoires are significantly less diverse than those of wildtype mice.
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Dntttm1Gfn/Dntttm1Gfn
involves: 129S2/SvPas * C57BL/6- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:14407)
- breed well and appear healthy, are of normal size, and do not have increased susceptibility to infection
- no marked abnormalities in the major T or B cell compartments and are capable of mounting T and B cell responses to complex antigens
- variable, diversity and joining segment so lymphocyte receptor genes from adult mutant mice have few template-independent nucleotides showing extensive evidence of homology-directed recombination
- no abnormal phenotype detected (MGI Ref ID J:14407)
Founder genetic background: 129S2/SvPas ES Cell Line
Strain genetic background: C57BL/6
Suggested Control Mice:
- C57BL/6
Research Applications
- Immunoglobulin and T cell receptor V(D)J recombination studies
- Human disease
- Immunology
Strain Origin
Donor: Dr. S. Gilfillan, Washington University School of Medicine
Primary Reference:
Gilfillan, S., A. Dierich, M. Lemeur, C. Benoist, and D. Mathis. Mice lacking TdT: mature animals with an immature lymphocyte repertoire. Science. 1993 Aug 27; 261(5125):1175-8. (Medline: 8356452)
Colony and Husbandry Information
Appearance
Coat color: Black
Other:
Breeding
MMRRC Breeding System: Sib mating
Breeding Scheme(s):
- Homozygous Female x Homozygous Male
Generation: N10+ prior to sibling inbreeding.
Overall Breeding Performance: Good
Reproductive Statistics
| Viability and Fertility: | Female | Male | |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | |
| Homozygotes are fertile: | Yes | Yes | |
| Heterozygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | 6 months | 6 months |
Average litter size: 6
Recommended wean age: 3 weeks
Special Considerations
None
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
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Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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