Strain Detail Sheet

Strain Name:
C57BL/6-Tg(ITGAL-Ptprca*)dCRasch/Mmucd
Stock Number:
046258-UCD
Citation ID:
RRID:MMRRC_046258-UCD
Other Names:
CD45-minigene transgenic ΔC (Delta C)

Strain Information

Gene Details

Ptprca
Name: protein tyrosine phosphatase receptor type C; a variant
Synonyms: CD45.1, Ly5a, PtprcSJL
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Transgenic
Tg(ITGAL-Ptprca*)dCRasch
Name: transgene insertion dC, William C Raschke
Type: Allele
Species: Multi-species
Chromosome: unknown
Alteration at locus: Transgenic
Ptprc
Name: protein tyrosine phosphatase receptor type C
Synonyms: T200, B220, CD45, Lyt-4, Ly-5
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Transgenic
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
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ITGAL
Name: integrin subunit alpha L
Type: Regulatory Region
Species: Homo sapiens (human)
Chromosome: 16
Alteration at locus: Transgenic
Genetic Alterations
This C57BL/6 strain carries a transgene expressing the full-length CD45 protein with a mutation that truncates the cytosolic domain to 12 post transmembrane amino acids. The mutation kills all CD45 tyrosine phosphatase activity in the protein produced from the transgene. The transgene, when transferred to the CD45 knockout background, will only produce CD45 lacking the functions of the cytosolic domain. The details of the transgene mutation are as follows:

  • gene: “CD45” = murine Ptprc (NCBI Gene ID: 19264)
  • mRNA RefSeq: NM_001111316.2 (longest isoform)
  • nucleic acid level alteration: NM_001111316.2:c.1802T>G
  • amino acid level alteration: NP_001104786.2:p.Leu601* - the minigene was altered to delete the cytosolic domain with only the 12 amino acids immediately carboxyl to the transmembrane domain remaining
Genotype Determination
Genotyping Protocol

Strain Description

Phenotype
CD45-minigene transgenic strain ΔC (DeltaC or "dC") is supplied on the C57BL/6 background and thus expresses both the transgene-encoded CD45 and the endogenous C57BL/6 CD45. When the ΔC transgene is transferred to the CD45 knockout C57BL/6 background (JAX Strain #002725), the mice express only a truncated CD45 protein with a deleted cytosolic domain and therefore no functions conveyed by the CD45 cytosolic domain, including protein tyrosine phosphatase activity. Strain ΔC on the CD 45 knockout background has the same severe immunodeficiency phenotype as the CD45 knockout strain, including T and B cell functional and maturational abnormalities. The ΔC strain on the knockout background also has the below average breeding characteristics of the CD45 knockout strain. Normal hematopoietic lineage and maturation-specific isoform regulation of the transgene product is maintained due to the inclusion of the natural introns between exons 3 and 9 in the transgene DNA sequence. The transgene encodes the CD45.1 allelic sequence which is detected by anti-CD45.1 antibodies.
MeSH Terms
  • Alleles
  • Animals
  • Antigens/metabolism
  • B-Lymphocytes/metabolism
  • Biocatalysis
  • Calcium/metabolism
  • Leukocyte Common Antigens/metabolism
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Models, Biological
  • Phenotype
  • RNA Splicing/genetics
  • Receptors, Antigen, B-Cell/metabolism
  • Sialic Acid Binding Ig-like Lectin 2/metabolism
  • Signal Transduction
  • T-Lymphocytes/metabolism
  • Thymocytes/metabolism
  • Transgenes
  • src-Family Kinases/metabolism
Strain Development
The CD45-minigene transgenic construct 'VGI-0020DC' which encodes a mutation in VGI-0020 (also known as LFAiT200 in reference PMID:12393487) to delete the cytosolic domain with only the 12 amino acids immediately carboxyl to the transmembrane region remaining.
Suggested Control Mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
  • Models for Human Disease

Strain Origin

Donor
William Raschke, Ph.D Virogenics
Primary Reference

Coughlin S, Noviski M, Mueller JL, Chuwonpad A, Raschke WC, Weiss A, ZikhermanJ. An extracatalytic function of CD45 in B cells is mediated by CD22. Proc NatlAcad Sci U S A. 2015 Nov 24;112(47):E6515-24. doi: 10.1073/pnas.1519925112. Epub 2015 Nov 11. (Medline PMID: 26561584)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black
Eye
Black
Other
CD45 minigene strain ΔC (Delta C) is one of a series of transgenic strains prepared with the CD45 minigene (MMRRC:44051, 46054, 46256, 46257, and 46258). The minigene was altered to delete the cytosolic domain with only the 12 amino acids immediately carboxyl to the transmembrane domain remaining. The resulting truncated CD45 protein is protein tyrosine phosphatase inactive. The ΔC strain has proven useful for studies of CD45 extracellular domain functions and molecular interactions with regard to lymphocyte activation and maturation. This strain should be also suitable for similar activation and maturation studies of other hematopoietic lineages which express CD45.

Note: The “ΔC” transgene locus is homozygous and on the C57BL/6 background. Thus, the rest of the entire genome, including the rest of the chromosome in which the transgene integrated, is C57BL/6. This means these mice express the transgene-encoded CD45.1 (PTPRCa) as well as the endogenous CD45.2 (PTPRCb) of C57BL/6. For most experimental purposes the “ΔC” transgene locus will need to be transferred to the CD45-null C57BL/6 background. This strain is not provided on the CD45-null background since these mice are poor breeders due to being immunodeficient. The strain is routinely maintained on the wild-type C57BL/6 background and bred onto the CD45 knockout background for experimental purposes.

Breeding

MMRRC Breeding System
Sib-mating
Generation
F25+
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Bred to Homozygosity
Yes
Average litter size
7-9
Recommended wean age
4 Weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
046258-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.