Strain Name:
BTBR.B6-Qkiqk-k2/Mmucd
Stock Number:
000056-UCD
Citation ID:
RRID:MMRRC_000056-UCD
Other Names:
Qkk2, Qkqk-k2

Strain Information

Qkiqk-k2
Name: quaking, KH domain containing RNA binding; quaking k2
Synonyms: qkk2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
Qki
Name: quaking, KH domain containing RNA binding
Synonyms: l(17)-1Wis, QkI, l17Wis1, 1110003F05Rik, Qk
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 19317
VEGA: 17
Homologene: 11059
Genetic Alterations
Offspring of an ENU-induced male. The qkk2 lesion is a T to A transversion in the KH(RNA binding) domain which results in a nonconservative amino acid change in a site predicted to be involved in protein-protein interaction.
Genotype Determination
Phenotype
Homozygous lethal at e9.5 - 11.5. due to failure to form large blood vessels due to abnormal smooth muscle differentiation.
Mammalian Phenotype Terms
Allelic Composition: Qkqk-k2/Qkqk-k2 (Genetic Background: involves: C57BL/6 * CBA/Ca )

Allelic Composition: Qke5/Qkqk-k2 (Genetic Background: involves: 101/Rl * C57BL/6J * CBA/Ca )

Allelic Composition: Qkqk-k2/Qkqk-kt1 (Genetic Background: involves: C57BL/6 * CBA/Ca * STOCK tw5 )

  • mortality/aging
Allelic Composition: Qkqk-k2/Qkqk-kt4 (Genetic Background: involves: C57BL/6 * CBA/Ca * STOCK tw5 )

MeSH Terms
  • Animals
  • Blood Vessels/embryology
  • Blood Vessels/physiology
  • Gene Expression Regulation, Developmental
  • Heart/embryology
  • Mice
  • Morphogenesis
  • RNA-Binding Proteins/genetics
  • RNA-Binding Proteins/physiology
  • Yolk Sac/blood supply
  • Yolk Sac/embryology
  • Yolk Sac/physiology
  • Yolk Sac/ultrastructure
Strain Development
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Birth Defects
Donor
Monica J. Justice, PhD, Baylor College of Medicine
Primary Reference
Noveroske JK, Lai L, Gaussin V, Northrop JL, Nakamura H, Hirschi KK, Justice MJ. Quaking is essential for blood vessel development. Genesis. 2002 Mar;32(3):218-30. (Medline PMID: 11892011)

Colony and Husbandry Information

Use high fat diet for mating cages if females start to lose pups due to cannibalism.

Please note: 
C57BL/6J donor females will be used for the recovery of frozen sperm at the listed recovery fee.
Additional fees will be required if BTBR donor females are requested for the recovery of frozen sperm please contact mmrrc@ucdavis.edu to discuss additional fees.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black back and tan underbelly
MMRRC Breeding System

Backcross to BTBRT+tf>/J or Backcross to C57BL/6J or Sib-mating

Please note: C57BL/6J donor females will be used for the recovery of frozen sperm at the listed recovery fee. Additional fees will be required if BTBR donor females are requested for the recovery of frozen sperm. Please contact mmrrc@ucdavis.edu to discuss additional fees.

Breeding Scheme(s)
  • BTBR T+ tf/J female x Heterozyous male or reciprocal mating
  • Heterozygous female x Heterozygous male sibling

or

  • C57BL/6J female x Heterozygous male or reciprocal mating
  • Heterozygous female x Heterozygous male sibling
Generation
N21 to BTBR (JAX 02282)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000056-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
000056-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.