Strain Detail Sheet

Strain Name:
FVB/NOve-TgTn(sb-Tyr)Tp1Ove/Mmmh
Stock Number:
065582-MU
Citation ID:
RRID:MMRRC_065582-MU

Strain Information

Gene Details

TgTn(sb-Tyr)Tp1Ove
Name: transgenic transposon concatemer TP1, Paul A Overbeek
Type: Allele
Species: Multi-species
Chromosome: 14
Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22173
Homologene: 30969
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sb
Name: sleeping beauty
Type: Transposon
Species: Multi-species
Chromosome:
Genetic Alterations
Chromosomal inversion on Chr14 during transgene integration leading to loss of Bmp4 expression in embryonic eyes. However, the inversion has not altered any sequences within the transcribed region of the gene. The donor assumes that the inversion disrupted (or positionally separated the promoter from) an enhancer that is required for Bmp4 transcription in the embryonic optic vesicle. In situ hybridization data (available on request - 65582_Data2.pdf) show loss of detectable Bmp4 transcripts in the optic vesicle of a homozygous mutant (-/-) embryo, compared to a +/- heterozygote control.
Genotype Determination

Strain Description

Phenotype
Anophthalmia, dark grey pigmentation. Reduced fertility, particularly for homozygote/homozygote matings. Homozygotes are slightly runted and have a darker coat color than heterozygotes.

Available on request (65582_Data1.jpg): Photograph showing 3 homozygotes (darker coat color and microphthalmia/aphakia/closed eyes) and 2 heterozygotes (lighter and slightly mottled coat color, open eyes of normal size).
Strain Development
FVB/N embryos were microinjected with a construct containing a tyrosinase minigene inserted into a modified version of the Sleeping Beauty transposon.
Suggested Control Mice
FVB/N

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Ocular morphogenesis

Strain Origin

Donor
Paul Overbeek, Ph.D., Baylor College of Medicine.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Homozygotes coat color is darker than that of the hets (see additional information under "Phenotype".

Breeding

MMRRC Breeding System
Sib-mating
Generation
F20
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced homozygous males and females sometimes die as newborns.
Homozygotes are fertile: Reduced Reduced Homozygous females and males are sometimes infertile. Line is best maintained by mating heterozygotes.
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
5 to 9
Recommended wean age
3 weeks
Average Pups Weaned
5 to 9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.