Allele Symbol: Msx1tm1Rilm
Name: targeted mutation 1, Richard Maas
Chromosome: 5; 21.0 cM
Alteration at locus: Knockout
A neo cassette was inserted into exon 2.
ES Cell Line: J1
All Msx1 -/- newborn mice die within 24 hours of birth. All Msx1 -/- manifest a cleft secondary palate, a deficiency of alveolar mandible and maxilla, and a failure of tooth development. These mice also exhibit abnormalities of the nasal, frontal, and parietal bones, and of the malleus in the middle ear.
Founder genetic background: 129S4/SvJae
Strain genetic background: BALB/c from ICR
Strain Development: BALB/c blastocysts injected with ES cells were transferred into pseudopregnant recipients. Male chimaeras, identified by agouti coat color, were mated with BALB/cTac females.
Suggested Control Mice:
Donor: Richard Maas, MD. PhD. Brigham and Women's Hospital / Harvard Medical School
Satokata I, Maas R. Msx1 deficient mice exhibit cleft palate and abnormalities of cranialfacial and tooth development. Nature Genetics. Apr 1994, v6, p348. (Medline PMID: 7914451)
Coat color: White
MMRRC Breeding System: Sib mating
Generation: N10 to Balb/cTac F2 As of: 4/26/2004
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male|
|Homozygotes are viable:||No||No|
|Homozygotes are fertile:||No||No|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||36 weeks||36 weeks|
Average litter size: 8
Recommended wean age: 3 weeks
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email firstname.lastname@example.org.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.)|
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH