Strain Name:
STOCK Adgra3tm1.1(HBEGF/EGFP,-cre/ERT2)Pac/Mmjax
Stock Number:
068344-JAX
Citation ID:
RRID:MMRRC_068344-JAX
Other Names:
Adgra3-cre/cre

Strain Information

Adgra3tm1.1(HBEGF/EGFP,-cre/ERT2)Pac
Name: adhesion G protein-coupled receptor A3; targeted mutation 1.1, Pam Cowin
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
cre/ERT2
Name: cre tamoxifen-dependent recombinase
Type: Gene
Species: Multi-species
Chromosome:
HBEGF
Name: heparin-binding EGF-like growth factor
Type: Gene
Species: Homo sapiens (human)
Chromosome: 5
Adgra3
Name: adhesion G protein-coupled receptor A3
Synonyms: 3830613O22Rik, Gpr125, Tem5-like
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70693
Homologene: 19235
Genetic Alterations
A cassette comprising the human diphtheria toxin receptor gene (HBEGF) fused to enhanced green fluorescent protein followed by self-cleaving peptide T2A and cre/ERT2 has been inserted immediately after the first methionine codon of the Adgra3 gene. This placed diphtheria toxin receptor, EGFP, and cre/ERT2 under the control of the Adgra3 promoter, and concurrently excised 502 bp encompassing 221 bp of exon 1 and part of the following intron to generate a knockout of Adgra3. The neomycin selection cassette has been removed by mating to an FLP-expressing line.
ES Cell Line
Not applicable
Phenotype
Adgra3cre/cre mice display a prominent eye phenotype with features of dry eye and blepharitis with full penetrance; this is best seen on FVB/N background. They show impairment of pubertal mammary ductal elongation. Approximately 30% of the males are subject to sudden sterility with age (7-8 mths) likely due to testicular stem cell exhaustion; those males may have sired a few litters earlier. 30% of the females show vaginal fusion, which presents around 6-8 weeks as a protrusion; affected females take on a superficial appearance of males due to fluid build-up from estrus secretions without route of escape.
Strain Development
The model was developed by Ingenious Targeting Laboratories: the ES cells were on a hybrid (129S6/SvEvTac x C57BL/6NTac) genetic background. Chimeras were mated to C57BL/6N once, and the neomycin cassette was removed by breeding to B6.Cg-Tg(ACTFLPe)9205Dym/J (RRID:IMSR_JAX:005703). The model was shipped to the donor, who bred the line to FVB/N once, then rederived using eggs from the F1 and sperm from B6-congenic tdTomato males B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (RRID:IMSR_JAX:007914). These were intercrossed to generate homozygotes for both Adgra3cre/cre and tdTomato, and maintained as such for many generations. The line was subsequently bred to Swiss Webster (Tac:SW) and the tdTomato allele was bred out.
Suggested Control Mice
Wild-type siblings, C57BL/6J, FVB/NJ, Swiss Webster
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
Donor
Pamela Cowin, Ph.D., New York University School of Medicine.
Primary Reference
In preparation, submitted, or in press

Colony and Husbandry Information

Homozygous pups are smaller and benefit from diet gel supplements between 2-3 weeks of age and later weaning ~24-28 day. The animals begin to breed a bit later than normal, at ~8-10 weeks. The eye phenotype becomes severe in males with age, and it waxes/wanes in females (likely with the hormonal cycle) becoming prominent during pregnancy. Their eyes do not respond to antibiotics - they have dry-eye that compensates with hyperlacrimation - often with only one eye affected.

In terms of breeding, in the donor's laboratory, Swiss Webster crosses were the best (used for embryo studies), followed by FVB/N (used for mammary studies). C57BL/6 were the least successful in terms of litter regularity and litter size.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Mixed background generate all colors above
Eye
Both
MMRRC Breeding System
Sib-mating
Generation
N?F?
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Reduced Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Variable Variable
Average litter size
7 to 9
Recommended wean age
4 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068344-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
068344-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.