Strain Name:
B6;129X1-Abca1tm2Jp/Mmmh
Stock Number:
009999-MU
Citation ID:
RRID:MMRRC_009999-MU

Strain Information

Abca1tm2Jp
Name: ATP-binding cassette, sub-family A member 1; targeted mutation 2, John S Parks
Synonyms: Abca1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Abca1
Name: ATP-binding cassette, sub-family A member 1
Synonyms: ABC1
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knockout
NCBI: 11303
HGNC: HGNC:29
Homologene: 21130
Genetic Alterations
A 1.65-kb polymerase chain reaction (PCR) generated fragment containing AbcA1 Exon 16, was inserted between the PGK terminator of the PGKNeo cassette and the double PGKtk cassettes of pPN2T at EcoRI and BarnHI sites. A 12-kb PCR-generated fragment from Intron 22 to Exon 30 was then inserted on the PGK promoter side of the PGKneo at blunted-Xhol and Notl sites to generate the final targeting vector. ESVJ ES cells from Genomesystems were electroporated with Notl Iinearized vector and subjected to G418 (180 ?g/mL) fialuridine (FIAU) (0.2 ?mol/L) selection for 7 days. ES cell colonies were screened for the targeting event by PCR. Correct targeted clones containing a deletion from exon17-22 (encoding nucleotide binding cassette 1) were then confirmed by Southern blot analysis.
Genotype Determination
ES Cell Line
WW6 derived from STOCK 129/Sv and C57BL/6J and SJL
Phenotype
Homozygous: Severe hypocholesterolemic, near complete absence of HDL, reduced levels of LDL and accumulation of foam cells.

Heterozygous: Moderately reduced levels of HDL, LDL
MeSH Terms
  • ATP Binding Cassette Transporter 1
  • ATP-Binding Cassette Transporters/genetics
  • Animals
  • Apolipoprotein A-I/genetics
  • Kidney/metabolism
  • Lipoproteins, HDL/blood
  • Lipoproteins, HDL/metabolism
  • Liver/metabolism
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Phosphatidylcholine-Sterol O-Acyltransferase/blood
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular
  • Models for Human Disease
  • Research Tools
Donor
Edward M. Rubin, M.D., Ph.D., Lawrence Berkeley National Laboratory
Primary Reference
Lee JY, Timmins JM, Mulya A, Smith TL, Zhu Y, Rubin EM, Chisholm JW, Colvin PL, Parks JS. HDLs in apoA-I transgenic Abca1 knockout mice are remodeled normally in plasma but are hypercatabolized by the kidney. J Lipid Res. 2005 Oct;46(10):2233-45. Epub 2005 Jul 16. (Medline PMID: 16024913)

Colony and Husbandry Information

Homozygous females are sterile.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
009999-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
009999-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
009999-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.