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Allele Symbol: Sgcatm575Kcam Name: sarcoglycan, alpha (dystrophin-associated glycoprotein) / (unnamed provisional allele) Alteration at locus: Targeted Mutation |
Genetic Alterations:
Two fragments carrying exon 1 and a portion of 5' and 3' contiguougus inton were
inserted into pPNT. A 9-Kb fragment including a portion of intron 3 and exon
4-exon 9 was inserted into XhoI-NotI cut plasmid. The correct targeting replaced
exon 2, exon 3, 571 bp of intron 1, and 65 bp of intron 3. This included a
neomycin resistance gene in opposite transcriptional orientation.
Genotype Determination:
ES Cell Line: R1
Phenotype:
Homozygote: Sgca-cull mice show phenotype similar to dystrophin-deficient mdx mice.
Pathology includes necrosis, fiber regeneration and central nucleation.
Atrophy, hypertrophy, fiber splitting, and endomysial fibrosis is also present
in Sgca-null muscle. Sgca-null mice older than 8 weeks of age show dystrophic
calcification associated with myocyte necrosis. Fatty infiltration was evident
in the mice.
Heterozygote: Do not show any morphological abnormalities.
Strain Type:
Founder genetic background: 129xC57BL/6
Strain genetic background: C57BL/6J
Strain Development:
Donor: Kevin P. Campbell, Ph.D., University of Iowa
Primary Reference:
Duclos F, Straub V, Moore SA, Venzke DP, Hrstka RF, Crosbie RH, Durbeej M, Lebakken CS, Ettinger AJ, van der Meulen J, Holt KH, Lim LE, Sanes JR, Davidson BL, Faulkner JA, Williamson R, Campbell KP. Progressive muscular dystrophy in alpha-sarcoglycan-deficient mice. J Cell Biol. 1998 Sep 21;142(6):1461-71. (Medline PMID: 9744877)
Sampaolesi M, Torrente Y, Innocenzi A, Tonlorenzi R, D'Antona G, Pellegrino MA, Barresi R, Bresolin N, De Angelis MG, Campbell KP, Bottinelli R, Cossu G. Cell therapy of alpha-sarcoglycan null dystrophic mice through intra-arterial delivery of mesoangioblasts. Science. 2003 Jul 25;301(5632):487-92. Epub 2003 Jul 10. (Medline PMID: 12855815)
Allamand V, Donahue KM, Straub V, Davisson RL, Davidson BL, Campbell KP. Early adenovirus-mediated gene transfer effectively prevents muscular dystrophy in alpha-sarcoglycan-deficient mice. Gene Ther. 2000 Aug;7(16):1385-91. (Medline PMID: 10981665)
Mice with decreased mobility may benefit from gruel as food/water source.
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
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Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 000425-MU-RESUS | Resuscitation from cryopreserved material | $1,963.00 | litter a |
| 000425-MU-SPERM | Cryopreserved spermatozoa | $424.00 | aliquot b |
| 000425-MU-EMBRYO | Cryopreserved embryo | $1,008.00 | aliquot c |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.
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Last Modified: January 11, 2010