Strain Detail Sheet
- NIH acquires knockout mice. Nature Genetics. 37:1167 (2005).
- Lexicon Genetics, Inc., "NIH initiative supporting placement of Lexicon Genetics, Inc. mice into public repositories" MGI Direct Data Submission 2005;():
ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.
MMRRC testing of the Lexicon ES Cell lines includes genotyping by PCR for verification of correct target, and mycoplasma and MPV testing.
Estimated timelines: The repository was sent only one or two vials per clone/cell line from the production center. Processing ES cells (including genetic verification) prior to shipping requires 6-8 weeks.
Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 029721-UCD-CELL | Lexicon ES cell line OST15553 | $964.08 / Non-Profit | Vial | Includes genetic confirmation |
| available service | Chromosome counts | $385.17 / Non-Profit | per clone | To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted. |
| additional products | Extra vial of cells for the same clone | $462.69 / Non-Profit | per clone | |
| additional products | Feeder cells - Irradiated | $148.67 / Non-Profit | per cell line |