Background
Beutler Strains are from the MUTAGENETIX archive of phenotypes and mutations produced in Dr. Bruce Beutler’s Lab (Center for the Genetics of Host Defense, The University of Texas Southwestern Medical Center). The Beutler strains have been produced by random N-ethyl-N-nitrosourea (ENU)-induced germline mutagenesis.
In the Beutler lab, male C57BL/6J mice, termed first generation (G1) mice, are mutagenized with ENU and their exomes sequenced. The G1 mice are subsequently propagated to the second (G2) and third generations (G3), whereby ENU-induced mutations will be homozygous reference (wild-type), heterozygous, or homozygous variant. The G3 mice are actively screened for immunological phenotypes. However, many non-immunological phenotypes (e.g. changes in pigmentation, metabolism, behavior, development morphology, or lethality) are often incidentally identified, and some of them address major biological questions. Mutations are positionally cloned more rapidly than they can be investigated in depth, and in many cases, more rapidly than they can be published in conventional journals. Therefore, all mutations in each G1 mouse are described on the MUTAGENETIX website, as are the essential phenotypic characteristics, when available.
All information on chromosomal locations and critical region intervals are currently based on Ensembl Mouse annotation (release 71). Specific sequence information (gene sequences, positions of mutations, etc.) is based on the NCBI GRCm38 (mm10) mouse assembly. Individual strain records on the MUTAGENETIX website contain links to specific cDNA sequences and/or genomic DNA sequences in the NCBI database.
MMRRC Holdings
Distribution
Strains are distributed using the MMRRC Conditions of Use *(COU). Mice are available only to investigators at non-profit institutions.
* The preceding link is to the COU text only; the COU web form will be provided via email after order submission. The email should be forwarded to your institutional official for completion.
Some Beutler strains were submitted as a sperm collection, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm of varying quality using techniques such as IVF and ICSI and are confident that we will have success in most if not all cases. However, because of the unknown quality of these sperm samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed for additional charges.
Citations
Information excerpted from the MUTAGENETIX website; additional information can be found there.
MUTAGENETIX should be cited in journal articles or on-line publications using the following conventions: {Authors, Science Writers, Beutler B}. Record for {allele name}, updated {date of last update}. MUTAGENETIX (TM), B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX. Accessed {date of download}. World Wide Web URL: http://mutagenetix.utsouthwestern.edu.
Citations should also acknowledge that these mice were received from the MMRRC, and mention RRID:MMRRC:0XXXX, where 0XXXX is replaced by the MMRRC ID number.
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