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MMRRC Submission Information

Start Submission Introduction Review Criteria Application Stage Strain Acceptance Strain Transfer Methods Types of Strain Submissions Contract Submissions Benefits of Strain Submission Material Transfer Agreements Special Arrangements Application Submission
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Strain Transfer Methods

The assigned MMRRC Center can accept a mouse strain as live mice, cryopreserved material, and/or embryonic stem (ES) cells.

Live Mice
Preferred transfer method

Coordinate with your strain’s assigned center to schedule the transfer. Mice should be of suitable breeding age.

Action: Contact your assigned center to arrange dates and shipping details.
Cryopreserved Material
Embryos or spermatozoa

Facilities can accept previously cryopreserved embryos and sperm. The Submitting Investigator must coordinate with the assigned MMRRC Center prior to transfer.

Risk: If only cryopreserved material is submitted, resuscitation may fail. The MMRRC cannot assure recoverability until material is successfully resuscitated to fertilized embryos or live mice.
Embryonic Stem (ES) Cells
Gene-targeted ES cells suitable for blastocyst injection

Please provide detailed information, such as:

  • Origin of the ES line and gene-targeting method
  • Passage number since electroporation
  • Genotyping confirming integration of the inserted allele
  • PCR protocol to confirm identity and (if relevant) homologous recombination
  • Nucleotide sequence of the inserted allele
  • Microinjection protocol and germline transmission info (if performed)

The Submitting Investigator must coordinate with the assigned MMRRC Center prior to transfer.

Risk: Conversion of ES cells to live, germline-transmitted mice can fail. Assurance is only possible after successful microinjection, chimera production, and germline transmission.
The assigned MMRRC Center will work with the Submitting Investigator to coordinate the transfer process for the selected method.