The biomedical research community is tackling the persistent challenge of reproducibility in animal studies head-on with a suite of new measures designed to enhance scientific rigor. Key among these measures are standardized nomenclature, improved experimental design, transparent reporting, data sharing, and centralized repositories. While the ARRIVE guidelines have made strides in setting documentation standards for laboratory animals, a critical gap remains: incomplete genetic information.

Enter the Laboratory Animal Genetic Reporting (LAG-R) framework. This innovative approach aims to thoroughly document the genetic makeup of animals used in scientific research, providing essential details that ensure experiments can be replicated accurately and models used appropriately. LAG-R focuses on comprehensive documentation of genetic backgrounds, modifications, and validation processes, which are crucial for interpreting and replicating experimental results.

The benefits of LAG-R are substantial. Standardizing genetic information documentation enhances the reproducibility of studies, making peer reviews more reliable and reducing the risks of misinterpretation. Additionally, LAG-R promotes data sharing, fostering collaboration across laboratories and institutions and ensuring consistent use of animal models with well-documented genetic backgrounds.

Furthermore, LAG-R supports the responsible use of research resources. The framework saves valuable time and resources by reducing redundant experiments and improving the ethical management of animal use through precise experimental planning. While verifying every genetic detail of research animals may be impractical, improved reporting and validation efforts will significantly boost research reliability.

This initiative marks a significant step towards ensuring the accuracy and dependability of animal studies, ultimately advancing scientific discovery. By enhancing transparency and thoroughness in genetic reporting, the LAG-R framework will support the development of more reliable and reproducible research models. This is a win for the entire scientific community, paving the way for groundbreaking advancements in biomedical research.


A recent study has made a significant breakthrough in understanding the mechanisms behind migraine headaches. It revealed the pivotal role of specific mouse models provided by the Mutant Mouse Resource & Research Centers (MMRRC). This research identifies the PACAP38-MrgprB2 pathway as a crucial factor in stress-induced migraine.

Study Overview

Migraine headaches, affecting approximately 15% of the global population, remain a poorly understood yet highly disruptive condition. Researchers have now demonstrated that increased pituitary adenylate cyclase-activating polypeptide-38 (PACAP38) due to stress can induce headache-like behaviors in mice. This discovery sheds light on how migraines may serve as a warning signal for stress-induced homeostatic imbalances.

Methodology and Key Findings

The study utilized PAC1 conditional knockout (KO) mice, generated by crossing C57BL/6N-Atm1BrdAdcyap1r1tm1a(KOMP)Wtsi/MbpMmucd mice (RRID:MMRRC_046500-UCD) with B6N(B6J)-Tg(CAG-Flpo)1Afst/Mmucd mice (RRID:MMRRC_036512-UCD). These mice were essential in investigating the PACAP38-MrgprB2 pathway.

Researchers found that increased levels of PACAP38, resulting from repetitive stress, caused MrgprB2-dependent headache behaviors. This effect was mediated by mast cell degranulation, which sensitized trigeminal ganglion neurons. Blocking this pathway successfully prevented the development of headache behaviors in the mice.

Implications for Migraine Treatment

This study highlights the PACAP38-MrgprB2 pathway as a promising target for new migraine treatments, particularly stress-related ones. Understanding this pathway provides critical insights into the biological mechanisms that cause migraines and opens new avenues for therapeutic development.


MMRRC Mice Used:

Contact Information

Customer Service:
Web Support:
US, Canada & Puerto Rico:
Nov 5 to Nov 9, 2024
Denver, CO
Booth TBD

Welcome to the Mutant Mouse Resource & Research Centers (MMRRC) Website

The MMRRC is the nation’s premier national public repository system for mutant mice. Funded by the NIH continuously since 1999, the MMRRC archives and distributes scientifically valuable spontaneous and induced mutant mouse strains and ES cell lines for use by the biomedical research community. The MMRRC consists of a national network of breeding and distribution repositories and an Informatics Coordination and Service Center located at 4 major academic centers across the nation. The MMRRC is committed to upholding the highest standards of experimental design and quality control to optimize the reproducibility of research studies using mutant mice. The MMRRC is supported by the Office of Research Infrastructure Programs (ORIP) in the Office of the Director at NIH. More than 60,000 mutant alleles are maintained as live mice, cryopreserved germplasm, and/or mutant ES cells. Live mice are supplied from a production colony, from a colony recovered from cryopreservation, or via micro-injection of ES cells. An MMRRC facility may offer cryopreserved material for resuscitation at the recipient scientist's institution.