Strain Name:
B6.129S6-Stat5a/Stat5btm2Mam/Mmjax
Stock Number:
032053-JAX
Citation ID:
RRID:MMRRC_032053-JAX

Gene Information

Allele Symbol: Stat5atm2Mam [MGI:3055318] (Mus musculus (mouse))
Name: signal transducer and activator of transcription 5A; targeted mutation 2, Lothar Hennighausen
Synonyms: Stat5fl, Stat5a/Stat5btm2Mam
Alteration at locus: Targeted Mutation
Gene Symbol: Stat5a [MGI:103036] (Mus musculus (mouse))
Name: signal transducer and activator of transcription 5A
Synonyms: STAT5
Chromosome: 11
Alteration at locus: Targeted Mutation
ES Cell Line
TC1/TC-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study cell proliferation, differentiation and survival in in any given cell type.

Heterozygous:
Not evaluated.
MeSH Terms
  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cell Division
  • Cell Survival
  • DNA/genetics
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Epithelial Cells/cytology
  • Epithelial Cells/metabolism
  • Female
  • Gene Targeting
  • Lactation/genetics
  • Lactation/metabolism
  • Mammary Glands, Animal/cytology
  • Mammary Glands, Animal/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Milk Proteins
  • Pregnancy
  • STAT5 Transcription Factor
  • Trans-Activators/deficiency
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
Strain Development
A targeting vector was designed to place Stat5a and Stat5b in a head to toe orientation with no genes between them. A loxP flanked neomycin resistance gene was inserted downstream of Stat5b and a loxP flanked hygromycin resistance gene was inserted downstream of Stat5a. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice for more than 10 generations. Correctly genotyped pups from each generation were selected using marker assisted genome scan analysis (speed congenic). Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Research tool
Donor
Lothar Hennighausen, Ph.D., National Institute of Diabetes and Digestive and Kidney Diseases/National Institutes of Health 
Primary Reference
1: Cui Y, Riedlinger G, Miyoshi K, Tang W, Li C, Deng CX, Robinson GW, Hennighausen L. Inactivation of Stat5 in mouse mammary epithelium during pregnancy reveals distinct functions in cell proliferation, survival, and differentiation. Mol Cell Biol. 2004 Sep;24(18):8037-47. (Medline PMID: 15340066)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as homozygotes.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032053-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.