Strain Detail Sheet

Strain Name    :

B6;129P2-Pde4dtm1Mct/Mmucd

Stock Number :

034404-UCD

Other Names   :

PDE4D, B6;129P2-Pde4dtm1Mct

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Pde4dtm1Mct
Name: targeted mutation 1, Marco Conti
Alteration at locus: Knockout
Gene Symbol: Pde4d
Name: phosphodiesterase 4D, cAMP specific
Chromosome: 13
Alteration at locus: Knockout

Genetic Alterations:
A 3.2 kb fragment encompassing exons 8 and 9 was replaced with a PGK-hrpt cassette inserted in the opposite orientation by homologous recombination.

Genotype Determination:

ES Cell Line: E14TG2a derived from 129P2/OlaHsd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Decreased body weight, postnatal growth retardation, increased cardiac muscle contractility, abnormal ovulation, reduced female fertility, decreased litter size.

Heterozygous phenotype: None.

Strain of Origin: 129P2/OlaHsd

Strain genetic background: C57BL/6;129P2/OlaHsd mixed backround

Strain Development: The targeting vectors were linearized with NotI and electroporated into E14TG2a embryonic stem (ES) cells. Homologous recombinant ES clones were screened by Southern blot analysis. ES cells from positive clones were microinjected into C57BL/6 blastocysts and implanted into pseudopregnant females. The resulting chimeras were mated to C57BL/6 mice to produce F1 progeny with heterozygous genotype (PDE4D+/−). Further crosses between the PDE4D+/− mice yielded F2 generation homozygous mutants (PDE4D−/−) with a mixed 129P2/Ola and C57BL/6 background.

Suggested Control Mice:

  • Wild-type littermates

Research Applications

  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Immunology and Inflammation
  • Models for Human Disease
  • Neurobiology
  • Reproduction

Strain Origin

Donor: Marco Conti, MD, University of California, San Francisco

Primary Reference:

  • Bruss MD; Richter W; Horner K; Jin SL; Conti M, Critical role of PDE4D in beta2-adrenoceptor-dependent cAMP signaling in mouse embryonic fibroblasts., J Biol Chem 2008 Aug 15;283(33):22430-42 (Medline PMID: 18508768)
  • Xiang Y; Naro F; Zoudilova M; Jin SL; Conti M; Kobilka B, Phosphodiesterase 4D is required for beta2 adrenoceptor subtype-specific signaling in cardiac myocytes., Proc Natl Acad Sci U S A 2005 Jan 18;102(3):909-14 (Medline PMID: 15644445)
  • Lehnart SE; Wehrens XH; Reiken S; Warrier S; Belevych AE; Harvey RD; Richter W; Jin SL; Conti M; Marks AR, Phosphodiesterase 4D deficiency in the ryanodine-receptor complex promotes heart failure and arrhythmias., Cell 2005 Oct 7;123(1):25-35 (Medline PMID: 16213210)

Colony and Husbandry Information

Special Considerations

Please note: Mixed background B6:129P2 mice are not commercially available.  Embryos were preserved using wild-type females from the colony as egg donors.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color:

A litter may contain a variety of colors including agouti, gray, albino, black or tan.

Other:

Breeding

MMRRC Breeding System: Random intra-strain mating or Backcross

Breeding Scheme(s):

  • Heterozygous female x Heterozygous male from the colony
  • Heterozygous female x Wild-type male from the colony or reciprocal mating
  • C57BL/6 female x Heterozygous male or reciprocal mating

Generation:

Overall Breeding Performance: Poor

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: 5 months 10 months

Average litter size: 5

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034404-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034404-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
034404-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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