Allele Symbol: Pde4dtm1Mct
Name: targeted mutation 1, Marco Conti
Alteration at locus: Knockout
Gene Symbol: Pde4d
Name: phosphodiesterase 4D, cAMP specific
Alteration at locus: Knockout
ES Cell Line: E14TG2a derived from 129P2/OlaHsd
Homozygous phenotype: Decreased body weight, postnatal growth retardation, increased cardiac muscle contractility, abnormal ovulation, reduced female fertility, decreased litter size.
Heterozygous phenotype: None.
Strain of Origin: 129P2/OlaHsd
Strain genetic background: B6;129P2
Strain Development: The targeting vectors were linearized with NotI and electroporated into E14TG2a embryonic stem (ES) cells. Homologous recombinant ES clones were screened by Southern blot analysis. ES cells from positive clones were microinjected into C57BL/6 blastocysts and implanted into pseudopregnant females. The resulting chimeras were mated to C57BL/6 mice to produce F1 progeny with heterozygous genotype (PDE4D+/−). Further crosses between the PDE4D+/− mice yielded F2 generation homozygous mutants (PDE4D−/−) with a mixed 129P2/Ola and C57BL/6 background.
Suggested Control Mice:
Donor: Marco Conti, MD, University of California, San Francisco
A litter may contain a variety of colors including agouti, gray, albino, black or tan.
MMRRC Breeding System: Random intra-strain mating or Backcross
Overall Breeding Performance: Poor
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||5 months||10 months|
Average litter size: 5
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.