Strain Name:
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Gene Information

Allele Symbol: Pde4dtm1Mct (Mus musculus (mouse))
Name: phosphodiesterase 4D, cAMP specific; targeted mutation 1, Marco Conti
Synonyms: PDE4D<->, 4DKO
Chromosome: 13
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:2451168
Gene Symbol: Pde4d (Mus musculus (mouse))
Name: phosphodiesterase 4D, cAMP specific
Synonyms: 9630011N22Rik, dunce, Dpde3
Chromosome: 13
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:99555
Genetic Alterations
A 3.2 kb fragment encompassing exons 8 and 9 was replaced with a PGK-hrpt cassette inserted in the opposite orientation by homologous recombination.
Genotype Determination
ES Cell Line
E14TG2a derived from 129P2/OlaHsd
Homozygous: Decreased body weight, postnatal growth retardation, increased cardiac muscle contractility, abnormal ovulation, reduced female fertility, decreased litter size.

Heterozygous: No observed phenotype.
Mammalian Phenotype Terms
Allelic Composition: Pde4dtm1Mct/Pde4dtm1Mct (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Pde4dtm1Mct/Pde4dtm1Mct (Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ )

Allelic Composition: Pde4dtm1Mct/Pde4dtm1Mct (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Strain Development
The targeting vectors were linearized with NotI and electroporated into E14TG2a embryonic stem (ES) cells. Homologous recombinant ES clones were screened by Southern blot analysis. ES cells from positive clones were microinjected into C57BL/6 blastocysts and implanted into pseudopregnant females. The resulting chimeras were mated to C57BL/6 mice to produce F1 progeny with heterozygous genotype (PDE4D+/−). Further crosses between the PDE4D+/− mice yielded F2 generation homozygous mutants (PDE4D−/−) with a mixed 129P2/Ola and C57BL/6 background.
Suggested Control Mice
  • Wild-type littermates
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Immunology and Inflammation
  • Models for Human Disease
  • Neurobiology
  • Reproduction
Marco Conti, M.D., University of California, San Francisco
Primary Reference
  • Bruss MD; Richter W; Horner K; Jin SL; Conti M, Critical role of PDE4D in beta2-adrenoceptor-dependent cAMP signaling in mouse embryonic fibroblasts., J Biol Chem. 2008 Aug 15;283(33):22430-42 (Medline PMID: 18508768)
  • Xiang Y; Naro F; Zoudilova M; Jin SL; Conti M; Kobilka B, Phosphodiesterase 4D is required for beta2 adrenoceptor subtype-specific signaling in cardiac myocytes., Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):909-14 (Medline PMID: 15644445)
  • Lehnart SE; Wehrens XH; Reiken S; Warrier S; Belevych AE; Harvey RD; Richter W; Jin SL; Conti M; Marks AR, Phosphodiesterase 4D deficiency in the ryanodine-receptor complex promotes heart failure and arrhythmias., Cell. 2005 Oct 7;123(1):25-35 (Medline PMID: 16213210)

Colony and Husbandry Informationon

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
Coat Color

A litter may contain a variety of colors including agouti, gray, albino, black, or chinchilla.

MMRRC Breeding System
Random intra-strain mating or Backcross
Breeding Scheme(s)
  • Heterozygous female x Heterozygous male from the colony
  • Heterozygous female x Wild-type male from the colony or reciprocal mating
  • Homozygous female x Homozygous male from within the colony
Overall Breeding Performance
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 5 months 10 months
Bred to Homozygosity
Average litter size
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
034404-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
034404-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
034404-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.