Strain Detail Sheet

Strain Name    :

FVB/N-Tg(Wap-Tsg101)4389Kuw/Mmmh

Stock Number :

030909-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Wap-Tsg101)4389Kuw
Name: transgene insertion 4389, Kay-Uwe Wagner
Alteration at locus: Transgenic
Promoter: Wap
Name: whey acidic protein
Alteration at locus: Transgenic
Transgene: Tsg101
Name: tumor susceptibility gene 101
Alteration at locus: Transgenic

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: None

Hemizygous phenotype: None, sopradic mammary cancer very late in life in a subset of animals.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Tg(Wap-Tsg101)4389Kuw/-
        FVB/N-Tg(Wap-Tsg101)4389Kuw
  • endocrine/exocrine gland phenotype
    • endocrine/exocrine gland phenotype (MGI Ref ID J:121475)
      • in contrast to cultured transgenic mammary epithelial cells which show susceptibility to toxicity by transgene expression, mammary tissue in lactating transgenic females appears normal when examined at day 16 of pregnancy and day 1 of lactation; mice can nurse and support their litters, similar to wild-type mice
      • abnormal mammary gland development (MGI Ref ID J:121475)
        • in addition to tumors, some females maintaine as parous mice (4/40), develop solid lesions before 18 months of age
        • tumor-free females (19) exhibit mammary gland abnormalities, with inflammatory nodules with squamous metaplasia and ectatic ducts found in almost all abnormal glands
        • atypical hyperplasia and squamous metaplasia are common lesions in mammary glands
        • some mammary glands of wild-type controls exhibit abnormal morphology resembling a mid-pregnant female
  • reproductive system phenotype
    • abnormal mammary gland development (MGI Ref ID J:121475)
      • in addition to tumors, some females maintaine as parous mice (4/40), develop solid lesions before 18 months of age
      • tumor-free females (19) exhibit mammary gland abnormalities, with inflammatory nodules with squamous metaplasia and ectatic ducts found in almost all abnormal glands
      • atypical hyperplasia and squamous metaplasia are common lesions in mammary glands
      • some mammary glands of wild-type controls exhibit abnormal morphology resembling a mid-pregnant female
  • tumorigenesis
    • mammary adenocarcinoma (MGI Ref ID J:121475)
      • some females maintained as parous mice for 12-24 months (3/40; 1 female had 3 tumors in thoracic mammary glands) develop palpable mammary tumors, identified as typical adenocarcinomas and on scirrhous carcinoma compared to only 1 palpable mammary tumor in wild-type controls at 20 months of age
  • integument phenotype
    • abnormal mammary gland development (MGI Ref ID J:121475)
      • in addition to tumors, some females maintaine as parous mice (4/40), develop solid lesions before 18 months of age
      • tumor-free females (19) exhibit mammary gland abnormalities, with inflammatory nodules with squamous metaplasia and ectatic ducts found in almost all abnormal glands
      • atypical hyperplasia and squamous metaplasia are common lesions in mammary glands
      • some mammary glands of wild-type controls exhibit abnormal morphology resembling a mid-pregnant female

Strain of Origin: FVB/N

Strain genetic background: FVB/N

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Cell Biology

Strain Origin

Donor: Kay-Uwe Wagner, Ph.D., University of Nebraska Medical Center

Primary Reference:

Oh KB, Stanton MJ, West WW, Todd GL, Wagner KU. Tsg101 is upregulated in a subset of invasive human breast cancers and its targeted overexpression in transgenic mice reveals weak oncogenic properties for mammary cancer initiation. Oncogene. 2007 Aug 30;26(40):5950-9. Epub 2007 Mar 19. (Medline PMID: 17369844)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030909-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030909-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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