Strain Name:
C57BL/6-Dnajc3tm8663Wcl/Mmmh
Stock Number:
010323-MU
Citation ID:
RRID:MMRRC_010323-MU
Major Collection:

Strain Information

Dnajc3tm8663Wcl
Name: DnaJ heat shock protein family (Hsp40) member C3; targeted mutation 8663, Warren C Ladiges
Synonyms: P58IPK-, P58IPK, Dnajc3tm1Wcl, P58-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Targeted Mutation
Dnajc3
Name: DnaJ heat shock protein family (Hsp40) member C3
Synonyms: p58IPK, mp58, Prkri, Dnajc3, Dnajc3a, Dnajc3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Targeted Mutation
NCBI: 100037258
VEGA: 14
HGNC: HGNC:9439
Homologene: 2486
Genetic Alterations
A mouse C57BL/6 genomic DNA library (Genome Systems, Inc.) in the pBeloBAC11 vector was screened using a restriction fragment probe corresponding to nucleotides -100 to +360 of the mouse P58^IPK cDNA. Based upon preliminary Southern blot analyses, a 15 kb HpaI fragment was subcloned, restriction mapped, and partially sequenced. This sequence was then assembled into an eGFP/ -galactosidase/PKG-Neo/pA targeting vector designed to replace P58^IPK exon 1 containing the translation start site. The targeting vector was transfected into C57BL/6-derived embryonic stem cells.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: Adult male P58^IPK null mice display glucosuria, hyperglycemia, and hypoinsulinemia. P58^IPK null mice show pancreatic cell depletion. Pancreatic cell failure in P58^IPK null mice is associated with increased apoptosis and upregulation of genes associated with apoptosis. P58^IPK null male mice are smaller and can be easily distinguished from heterozygous and wild type mice as early as two weeks of age.

Heterozgyous: Not characterized 
Mammalian Phenotype Terms
Allelic Composition: Dnajc3tm8663Wcl/Dnajc3tm8663Wcl (Genetic Background: C57BL/6-Dnajc3tm8663Wcl/Mmmh )

MeSH Terms
  • Animals
  • Apoptosis/physiology
  • Diabetes Mellitus/genetics
  • Diabetes Mellitus/physiopathology
  • Endoplasmic Reticulum/genetics
  • Endoplasmic Reticulum/physiology
  • Female
  • Glycosuria
  • HSP40 Heat-Shock Proteins
  • Hyperglycemia
  • Insulin/metabolism
  • Islets of Langerhans/physiopathology
  • Male
  • Mice
  • Mice, Knockout
  • Molecular Chaperones/genetics
  • Molecular Chaperones/physiology
  • Mutation
  • Repressor Proteins/genetics
  • Repressor Proteins/physiology
  • Up-Regulation
Strain Development
The contribution of these stem cells to the germline of chimeric mice was assessed by breeding with C57BL/6 females and screening for black offspring. Heterozygous mice were identified by PCR and intercrossed to obtain a Mendelian ratio of homozygous offspring. Routine genotyping for homozygosity was done by PCR using primers specific for an internal targeting deletion sequence (flanking the 59 bp Xmal deletion in the targeted P58^IPK allele) and an external genomic sequence. Mice were 100% C57BL/6 (from a C57BL/6 ES cell line), and were maintained on the C57BL/6 background by alternate generation backcrossing. Tissues and embryonic fibroblasts derived from homozygous, heterozygous, and wild-type littermates were processed and subjected to Western blot analysis. All progeny displayed the expected Mendelian ratio of heterozygous versus homozygous genotypes.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Metabolism
  • Models for Human Disease
  • Research Tools
Donor
Warren C. Ladiges, DVM, University of Washington
Primary Reference
Ladiges WC, Knoblaugh SE, Morton JF, Korth MJ, Sopher BL, Baskin CR, MacAuley A, Goodman AG, LeBoeuf RC, Katze MG. Pancreatic beta-cell failure and diabetes in mice with a deletion mutation of the endoplasmic reticulum molecular chaperone gene P58IPK. Diabetes. 2005 Apr;54(4):1074-81. (Medline PMID: 15793246)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
010323-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
010323-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
010323-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.