Strain Name:
STOCK Chek2tm1Pjs/Mmnc
Stock Number:
014111-UNC
Citation ID:
RRID:MMRRC_014111-UNC
Major Collection:

Gene Information

Allele Symbol: Chek2tm1Pjs (Mus musculus (mouse))
Name: checkpoint kinase 2; targeted mutation 1, Peter J Stambrook
Synonyms: Chk2*1100delC
Chromosome: 5
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:3812090
Gene Symbol: Chek2 (Mus musculus (mouse))
Name: checkpoint kinase 2
Synonyms: CHK2, Rad53
Chromosome: 5
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:1355321
Genetic Alterations
Floxed neo was inserted into exon 9, removed using Cre Recombinase in EIIa mice, leaving a LoxP site. The cytosine coding in position 1113 of mRNA sequence is deleted. This site is analogous to the human position 1100.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype
    Homozygous: Not characterized

    Heterozygous:
     Not characterized
    Mammalian Phenotype Terms
    Allelic Composition: Chek2tm1Pjs/Chek2tm1Pjs (Genetic Background: involves: 129/Sv * Black Swiss * FVB/N )

    MeSH Terms
    • Alleles
    • Animals
    • Cell Cycle
    • Cells, Cultured
    • Checkpoint Kinase 2
    • DNA Damage
    • Female
    • Genetic Predisposition to Disease
    • Genomic Instability
    • Homozygote
    • Mammary Neoplasms, Experimental/genetics
    • Mice
    • Mice, Transgenic
    • Polymorphism, Genetic
    • Protein-Serine-Threonine Kinases/genetics
    • Protein-Serine-Threonine Kinases/metabolism
    • RNA Stability
    • RNA, Messenger/metabolism
    • 9,10-Dimethyl-1,2-benzanthracene
    • Blotting, Western
    • Fibroblasts/metabolism
    • Gene Deletion
    • Genetic Predisposition to Disease/genetics
    • Genotype
    • Immunohistochemistry
    • Male
    • Neoplasms/chemically induced
    • Neoplasms/genetics
    • Neoplasms/pathology
    • Phosphorylation
    • Polymorphism, Single Nucleotide
    • Risk Factors
    • Sex Factors
    • Time Factors
    • cdc25 Phosphatases/genetics
    • cdc25 Phosphatases/metabolism
    • Breast Neoplasms/epidemiology
    • Breast Neoplasms/genetics
    • Breast Neoplasms/pathology
    • Cell Division
    • Cell Line, Tumor
    • Genetic Variation
    • Humans
    • Mammary Neoplasms, Animal/genetics
    • Mammary Neoplasms, Animal/pathology
    • Receptor Protein-Tyrosine Kinases/genetics
    • Sequence Deletion
    Strain Development
    Mice were crossed with EIIa (mice that express Cre Recombinase in the permanent line) in order to remove the floxed Neomycin resistance gene. Further crosses were with C57BL/6 and Black Swiss mice
    Suggested Control Mice
    • Wildtype littermates
    • Apoptosis
    • Cancer
    • Cell Biology
    • Internal/Organ
    • Models for Human Disease
    Donor
    Peter J. Stambrook, Ph.D., University of Cincinnati
    Primary Reference
    • Bahassi el M; Penner CG; Robbins SB; Tichy E; Feliciano E; Yin M; Liang L; Deng L; Tischfield JA; Stambrook PJ, The breast cancer susceptibility allele CHEK2*1100delC promotes genomic instability in a knock-in mouse model., Mutat Res 2007 Mar 1;616(1-2):201-9 (Medline PMID: 17174984)
    • Bahassi el M; Robbins SB; Yin M; Boivin GP; Kuiper R; van Steeg H; Stambrook PJ, Mice with the CHEK2*1100delC SNP are predisposed to cancer with a strong gender bias., Proc Natl Acad Sci U S A 2009 Oct 6;106(40):17111-6 (Medline PMID: 19805189)
    • Meyer SE; Peace BE; Bahassi el M; Kavanaugh GM; Wagh PK; Robbins SB; Yin M; Wells SI; Zinser GM; Stambrook PJ; Waltz SE, Chk2*1100delC Acts in synergy with the Ron receptor tyrosine kinase to accelerate mammary tumorigenesis in mice., Cancer Lett 2010 Oct 28;296(2):186-93 (Medline PMID: 20434834)

    Colony and Husbandry Informationon

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    014111-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / $2,263.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3 : 20-40 embryos / aliquot
    014111-UNC-SPERM Cryo-preserved spermatozoa $548.00 / $910.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2
    014111-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / $4,696.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.