Strain Detail Sheet

Strain Name    :

STOCK Chek2tm1Pjs/Mmnc

Stock Number :

014111-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Chek2tm1Pjs
Name: targeted mutation 1, Peter J Stambrook
Alteration at locus: Targeted Mutation
Gene Symbol: Chek2
Name: checkpoint kinase 2
Chromosome: 5
Alteration at locus: Targeted Mutation

Genetic Alterations:
Floxed neo was inserted into exon 9, removed using Cre Recombinase in EIIa mice, leaving a LoxP site. The cytosine coding in position 1113 of mRNA sequence is deleted. This site is analogous to the human position 1100.

Genotype Determination:

  • Donor's Protocol
  • Center protocol and contact for technical support will be shipped with mice.

ES Cell Line: Not specified derived from 129S6/SvEvTac

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: No known phenotype.

Heterozygous phenotype: No known phenotype.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Chek2tm1Pjs/Chek2tm1Pjs
        involves: 129/Sv * Black Swiss * FVB/N
  • cellular phenotype
    • abnormal cell physiology (MGI Ref ID J:118262)
      • mice embryonic fibroblasts exhibit constitutively activated DNA damage response
      • abnormal cell cycle (MGI Ref ID J:118262)
        • mouse embryonic fibroblasts exhibit fewer cells with G1 DNA content, increased number of cells in S-phase and increased cells with G2 DNA content compared to wild-type cells whether or not cells are irradiated
    • polyploidy (MGI Ref ID J:118262)
      • 8% of mouse embryonic fibroblasts are polyploidy

Strain of Origin: 129S6/SvEvTac

Strain genetic background: 129 SVEV/Tac C57/B16 Black Swiss/FVBN

Strain Development: Mice were crossed with EIIa (mice that express Cre Recombinase in the permanent line) in order to remove the floxed Neomycin resistance gene. Further crosses were with C57BL/6 and Black Swiss mice

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Internal/Organ
  • Models for Human Disease

Strain Origin

Donor: Peter J. Stambrook, Ph.D., University of Cincinnati

Primary Reference:

  • Bahassi el M; Penner CG; Robbins SB; Tichy E; Feliciano E; Yin M; Liang L; Deng L; Tischfield JA; Stambrook PJ, The breast cancer susceptibility allele CHEK2*1100delC promotes genomic instability in a knock-in mouse model., Mutat Res 2007 Mar 1;616(1-2):201-9 (Medline PMID: 17174984)
  • Bahassi el M; Robbins SB; Yin M; Boivin GP; Kuiper R; van Steeg H; Stambrook PJ, Mice with the CHEK2*1100delC SNP are predisposed to cancer with a strong gender bias., Proc Natl Acad Sci U S A 2009 Oct 6;106(40):17111-6 (Medline PMID: 19805189)
  • Meyer SE; Peace BE; Bahassi el M; Kavanaugh GM; Wagh PK; Robbins SB; Yin M; Wells SI; Zinser GM; Stambrook PJ; Waltz SE, Chk2*1100delC Acts in synergy with the Ron receptor tyrosine kinase to accelerate mammary tumorigenesis in mice., Cancer Lett 2010 Oct 28;296(2):186-93 (Medline PMID: 20434834)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
014111-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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