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Gene Information

Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Spyros Artavanis-Tsakonas
Synonyms: WT76
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Knock-In
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrosa26, Gtrgeo26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
NCBI: 14910
Name: notch 3
Type: Gene
Species: Homo sapiens (human)
Chromosome: 19
Alteration at locus: Knock-In
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Homozygous: ROSA NOTCH3-WT76 mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the human NOTCH3 is blocked by an upstream loxP-flanked STOP sequence. When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present, resulting in human NOTCH3 expression and the parallel expression of enhanced green fluorescent protein (GFP) targeted to the nucleus. Expression of NOTCH3 in vascular smooth muscle cells rescues the stroke susceptibility phenotype of Notch3 knockout mice.

Heterozygote: Not evaluated

Mammalian Phenotype Terms
Allelic Composition: Gt(ROSA)26Sortm1(NOTCH3)Sat/Gt(ROSA)26Sor+ (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

MeSH Terms
  • Alleles
  • Animals
  • Arterioles/pathology
  • Brain/blood supply
  • Cerebrovascular Disorders/etiology
  • Disease Models, Animal
  • Humans
  • Ischemia
  • Mice
  • Mutation, Missense
  • Receptor, Notch3
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism
  • Signal Transduction/physiology
  • Transgenes
  • Aorta/pathology
  • Aorta/ultrastructure
  • Arteries/pathology
  • Arteries/ultrastructure
  • Brain/pathology
  • Brain Ischemia/complications
  • Brain Ischemia/metabolism
  • Brain Ischemia/pathology
  • Cell Separation
  • Disease Susceptibility
  • Flow Cytometry
  • Male
  • Mice, Knockout
  • Muscle, Smooth, Vascular/metabolism
  • Muscle, Smooth, Vascular/pathology
  • Myocytes, Smooth Muscle/metabolism
  • Myocytes, Smooth Muscle/pathology
  • Regional Blood Flow
  • Signal Transduction
  • Stroke/complications
  • Stroke/metabolism
  • Stroke/pathology
Strain Development
A targeting vector containing a splice acceptor site, a loxP-flanked PGK neomycin cassette, a human NOTCH3 cDNA and an IRES-nuclear EGFP sequence was inserted into the GT(ROSA)26Sor locus. The construct was introduced into 129S6/SvEvTac -derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting "WT76" chimeric animals were crossed to 129S2/SvEv mice. The mice were crossed to 129S2/SvEv for an unknown number of generations. Upon arrival, mice were bred to 129S1/SvImJ for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease
  • Neurobiology
  • Research Tools
Spyros Artavanis-Tsakonas, Ph.D., Harvard Medical School
Primary Reference
  • Arboleda-Velasquez JF; Manent J; Lee JH; Tikka S; Ospina C; Vanderburg CR; Frosch MP; Rodriguez-Falcon M; Villen J; Gygi S; Lopera F; Kalimo H; Moskowitz MA; Ayata C; Louvi A; Artavanis-Tsakonas S, PNAS Plus: Hypomorphic Notch 3 alleles link Notch signaling to ischemic cerebral small-vessel disease., Proc Natl Acad Sci U S A 2011 May 24;108(21):E128-35 (Medline PMID: 21555590)
  • Arboleda-Velasquez JF; Zhou Z; Shin HK; Louvi A; Kim HH; Savitz SI; Liao JK; Salomone S; Ayata C; Moskowitz MA; Artavanis-Tsakonas S, Linking Notch signaling to ischemic stroke., Proc Natl Acad Sci U S A 2008 Mar 25;105(12):4856-61 (Medline PMID: 18347334)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as homozygotes.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032998-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
032998-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.