Strain Detail Sheet

Strain Name    :

B6.Cg-Sod1tm1Cje/Mmmh

Stock Number :

000203-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Sod1tm1Cje
Name: targeted mutation 1, Charles J Epstein
Alteration at locus: Knockout
Gene Symbol: Sod1
Name: superoxide dismutase 1, soluble
Chromosome: 16
Alteration at locus: Knockout

Genetic Alterations:
The 3rd and 4th exons of Sod1 were replaced with CITE-neo.

Genotype Determination:

ES Cell Line: CB1-4Ê derived from C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

The heterozygous mutants have no overt phenotype. The homozygous mutants develop hepatocellular carcinoma by 20 months of age and have an average lifespan of 20 months.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Sod1tm1Cje/Sod1tm1Cje
        involves: C57BL/6J * CD-1
  • cellular phenotype
    • decreased cell proliferation (MGI Ref ID J:42265)
      • cultured fibroblasts from homozygous mice did not grow well in culture and exhibited a growth rate of only 25% of controls
    • increased apoptosis (MGI Ref ID J:42265)
      • cultured fibroblasts from homozygous mice undergo apoptosis at 4-10 times control cultures

Strain of Origin: C57BL/6J x (RB(11.16)2H x Rb(16.17)32Lub)F1

Strain genetic background: C57BL/6

Strain Development: See primary reference.

Suggested Control Mice:

  • Wildtype littermates
  • C57BL/6

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Diabetes/Obesity
  • Models for Human Disease

Strain Origin

Donor: Ting-Ting Huang, Ph.D., Stanford University

Primary Reference:

  • Huang TT, Yasunami M, Carlson EJ, Gillespie AM, Reaume AG, Hoffman EK, Chan PH, Scott RW, Epstein CJ. Superoxide-mediated cytotoxicity in superoxide dismutase-deficient fetal fibroblasts. Arch Biochem Biophys. 1997 Aug 15;344(2):424-32. (Medline PMID: 9264557)
  • Kondo T, Reaume AG, Huang TT, Carlson E, Murakami K, Chen SF, Hoffman EK, Scott RW, Epstein CJ, Chan PH. Reduction of CuZn-superoxide dismutase activity exacerbates neuronal cell injury and edema formation after transient focal cerebral ischemia. J Neurosci. 1997 Jun 1;17(11):4180-9. (Medline PMID: 9151735)
  • Kondo T, Reaume AG, Huang TT, Murakami K, Carlson E, Chen S, Scott RW, Epstein CJ, Chan PH. Edema formation exacerbates neurological and histological outcomes after focal cerebral ischemia in CuZn-superoxide dismutase gene knockout mutant mice. Acta Neurochir Suppl. 1997;70:62-4. (Medline PMID: 9416279)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color:

Other:


Breeding

MMRRC Breeding System: Non inbred

Breeding Scheme(s):

  • Heterozgyous female x C57BL/6 male

Generation: Unknown

Overall Breeding Performance: Normal

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 16 weeks 16 weeks

Average litter size: 6 - 8

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000203-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000203-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
000203-MU-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/23/2014