Strain Name:
B6.Cg-Sod1tm1Cje/Mmmh
Stock Number:
000203-MU
Citation ID:
RRID:MMRRC_000203-MU

Strain Information

Sod1tm1Cje
Name: superoxide dismutase 1, soluble; targeted mutation 1, Charles J Epstein
Synonyms: SOD1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
Sod1
Name: superoxide dismutase 1, soluble
Synonyms: Ipo1, Sod-1, CuZnSOD, Cu/Zn-SOD, Cu(2+)-Zn2+ superoxide dismutase, B430204E11Rik, Ipo-1, SODC
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Knockout
NCBI: 20655
VEGA: 16
Homologene: 392
Genetic Alterations
The 3rd and 4th exons of Sod1 were replaced with CITE-neo.
Genotype Determination
ES Cell Line
CB1-4 derived from C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1
Phenotype
The heterozygous mutants have no overt phenotype. The homozygous mutants develop hepatocellular carcinoma by 20 months of age and have an average lifespan of 20 months.
Mammalian Phenotype Terms
Allelic Composition: Sod1tm1Cje/Sod1tm1Cje (Genetic Background: involves: C57BL/6J * CD-1 )

MeSH Terms
  • Animals
  • Apoptosis
  • Blotting, Southern
  • Catalase/metabolism
  • Cell Division
  • Cell Survival/drug effects
  • Cells, Cultured
  • Fibroblasts
  • Gene Targeting
  • Glutathione Peroxidase/metabolism
  • Mice
  • Mice, Knockout
  • Paraquat/pharmacology
  • Sequence Deletion
  • Superoxide Dismutase/deficiency
  • Superoxide Dismutase/genetics
  • Superoxide Dismutase/metabolism
  • Superoxides/toxicity
  • Apoptosis/physiology
  • Biotin
  • Blood-Brain Barrier/physiology
  • Cell Death/physiology
  • Cerebral Infarction/physiopathology
  • DNA Fragmentation
  • Deoxyuracil Nucleotides
  • Edema/physiopathology
  • Evans Blue/pharmacokinetics
  • Ischemic Attack, Transient/enzymology
  • Ischemic Attack, Transient/mortality
  • Ischemic Attack, Transient/physiopathology
  • Mice, Mutant Strains
  • Neurologic Examination
  • Neurons/enzymology
  • Neurons/pathology
  • Oxidative Stress/physiology
  • Prosencephalon/blood supply
  • Prosencephalon/enzymology
  • Prosencephalon/pathology
  • Reactive Oxygen Species/physiology
  • Staining and Labeling
  • Arterial Occlusive Diseases/pathology
  • Brain Edema/genetics
  • Brain Edema/pathology
  • Disease Models, Animal
  • Heterozygote
  • Homozygote
  • Ischemic Attack, Transient/genetics
  • Ischemic Attack, Transient/pathology
  • Superoxides/metabolism
Strain Development
See primary reference.
Suggested Control Mice
  • Wildtype littermates
  • C57BL/6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cell Biology
  • Diabetes/Obesity
  • Models for Human Disease
Donor
Ting-Ting Huang, Ph.D., Stanford University
Primary Reference
  • Huang TT, Yasunami M, Carlson EJ, Gillespie AM, Reaume AG, Hoffman EK, Chan PH, Scott RW, Epstein CJ. Superoxide-mediated cytotoxicity in superoxide dismutase-deficient fetal fibroblasts. Arch Biochem Biophys. 1997 Aug 15;344(2):424-32. (Medline PMID: 9264557)
  • Kondo T, Reaume AG, Huang TT, Carlson E, Murakami K, Chen SF, Hoffman EK, Scott RW, Epstein CJ, Chan PH. Reduction of CuZn-superoxide dismutase activity exacerbates neuronal cell injury and edema formation after transient focal cerebral ischemia. J Neurosci. 1997 Jun 1;17(11):4180-9. (Medline PMID: 9151735)
  • Kondo T, Reaume AG, Huang TT, Murakami K, Carlson E, Chen S, Scott RW, Epstein CJ, Chan PH. Edema formation exacerbates neurological and histological outcomes after focal cerebral ischemia in CuZn-superoxide dismutase gene knockout mutant mice. Acta Neurochir Suppl. 1997;70:62-4. (Medline PMID: 9416279)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
MMRRC Breeding System
Non inbred
Breeding Scheme(s)
Heterozgyous female x C57BL/6 male
Generation
Unknown
Overall Breeding Performance
Normal
Average litter size
6 - 8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000203-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000203-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
000203-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.