Strain Name:
B6.129-Dnajc6tm1Legr/Mmjax
Stock Number:
036980-JAX
Citation ID:
RRID:MMRRC_036980-JAX
Other Names:
auxilin knockout mouse,

Gene Information

Allele Symbol: Dnajc6tm1Legr (Mus musculus (mouse))
Name: DnaJ heat shock protein family (Hsp40) member C6; targeted mutation 1, Lois Greene
Synonyms: Dnajc6
Chromosome: 4
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:4440489
Gene Symbol: Dnajc6 (Mus musculus (mouse))
Name: DnaJ heat shock protein family (Hsp40) member C6
Synonyms: 2810027M23Rik, auxilin
Chromosome: 4
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:1919935
Genetic Alterations
The vector targeted the introns surrounding exon3 of Dnaj6. Neomycin was inserted by recombination into this chromosomal locus
Genotype Determination
ES Cell Line
GSI-1 derived from 129X1/SvJ
Phenotype

Homozygous: low birth weight, high mortality at birth, fertility problems

Heterozygous: none

Cre-excised Phenotype: undetermined

Mammalian Phenotype Terms
Allelic Composition: Dnajc6tm1Legr/Dnajc6tm1Legr (Genetic Background: B6.129-Dnajc6tm1Legr )

Allelic Composition: Dnajc6tm1Legr/Dnajc6+ (Genetic Background: B6.129-Dnajc6tm1Legr )

MeSH Terms
  • Animals
  • Auxilins/genetics
  • Auxilins/physiology
  • Clathrin/metabolism
  • Endocytosis
  • Female
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nerve Endings/metabolism
  • Protein-Serine-Threonine Kinases/metabolism
  • Synapses/metabolism
  • Up-Regulation
  • Adolescent
  • Auxilins/metabolism
  • Base Sequence
  • DNA Mutational Analysis/methods
  • Family Health
  • HSP40 Heat-Shock Proteins/genetics
  • HSP40 Heat-Shock Proteins/metabolism
  • Humans
  • Male
  • Molecular Chaperones/genetics
  • Molecular Chaperones/metabolism
  • Mutation
  • Neurons/metabolism
  • Parkinsonian Disorders/genetics
  • Pedigree
Strain Development
Gene targeted GSI 129/SV embryonic stem cells injected into C57BL/6 blastocysts to generate chimeras. Chimeras crossed to C57BL/6 mice to generate heterozygotes, the linearized targeting construct was then introduced into the embryonic stem cell line GSI 129/SV. The transfectants were rst selected by a G418 resistance cassette, followed by Southern blot analysis to obtain the positive clones (Fig. S1B). Genotyping of the offspring was performed by Southern blot analysis or PCR using DNA from tail snips. For the experiments reported in the study, we backcrossed the S129 heterozygote mutant mice with wild-type C57BL/6 mice to completely transfer the genotype from the S129 mice strain to the C57BL/6 strain. After eight backcrossings, heterozygote mice were mated to obtain homozygote auxilin knockout mice.


Suggested Control Mice
Wild-type littermates
Donor
Lois Greene, Ph.D., National Insitutes of Health.
Primary Reference
  • Yim YI, Sun T, Wu LG, Raimondi A, De Camilli P, Eisenberg E, Greene LE. Endocytosis and clathrin-uncoating defects at synapses of auxilin knockout mice. Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4412-7. doi: 10.1073/pnas.1000738107. Epub 2010 Feb 16. (Medline PMID: 20160091)
  • Edvardson S, Cinnamon Y, Ta-Shma A, Shaag A, Yim YI, Zenvirt S, Jalas C, Lesage S, Brice A, Taraboulos A, Kaestner KH, Greene LE, Elpeleg O. A deleterious mutation in DNAJC6 encoding the neuronal-specific clathrin-uncoating co-chaperone auxilin, is associated with juvenile parkinsonism. PLoS One. 2012;7(5):e36458. doi: 10.1371/journal.pone.0036458. Epub 2012 May 1. (Medline PMID: 22563501)

Colony and Husbandry Informationon

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • use harem breeding because some females have fertility problems
Generation
N20
Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Reduced Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 months 10 months
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036980-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity2
036980-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.