Strain Detail Sheet

Strain Name:
B6.129-Dnajc6tm1Legr/Mmjax
Stock Number:
036980-JAX
Citation ID:
RRID:MMRRC_036980-JAX
Other Names:
auxilin knockout mouse,

Strain Information

Gene Details

Dnajc6tm1Legr
Name: DnaJ heat shock protein family (Hsp40) member C6; targeted mutation 1, Lois Greene
Synonyms: Dnajc6tm1Pdc
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Dnajc6
Name: DnaJ heat shock protein family (Hsp40) member C6
Synonyms: auxilin, 2810027M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
NCBI: 72685
Homologene: 8865
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Genetic Alterations
The vector targeted the introns surrounding exon3 of Dnaj6. Neomycin was inserted by recombination into this chromosomal locus
Genotype Determination
ES Cell Line
GSI-1 derived from 129X1/SvJ

Strain Description

Phenotype

Homozygous: low birth weight, high mortality at birth, fertility problems

Heterozygous: none

Cre-excised Phenotype: undetermined

Mammalian Phenotype Terms
Allelic Composition: Dnajc6tm1Legr/Dnajc6tm1Legr (Genetic Background: B6.129-Dnajc6tm1Legr )
Allelic Composition: Dnajc6tm1Legr/Dnajc6+ (Genetic Background: B6.129-Dnajc6tm1Legr )
MeSH Terms
  • Animals
  • Auxilins/genetics
  • Auxilins/physiology
  • Clathrin/metabolism
  • Endocytosis
  • Female
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nerve Endings/metabolism
  • Protein-Serine-Threonine Kinases/metabolism
  • Synapses/metabolism
  • Up-Regulation
  • Adolescent
  • Auxilins/metabolism
  • Base Sequence
  • DNA Mutational Analysis/methods
  • Family Health
  • HSP40 Heat-Shock Proteins/genetics
  • HSP40 Heat-Shock Proteins/metabolism
  • Humans
  • Male
  • Molecular Chaperones/genetics
  • Molecular Chaperones/metabolism
  • Mutation
  • Neurons/metabolism
  • Parkinsonian Disorders/genetics
  • Pedigree
Strain Development
Gene targeted GSI 129/SV embryonic stem cells injected into C57BL/6 blastocysts to generate chimeras. Chimeras crossed to C57BL/6 mice to generate heterozygotes, the linearized targeting construct was then introduced into the embryonic stem cell line GSI 129/SV. The transfectants were rst selected by a G418 resistance cassette, followed by Southern blot analysis to obtain the positive clones (Fig. S1B). Genotyping of the offspring was performed by Southern blot analysis or PCR using DNA from tail snips. For the experiments reported in the study, we backcrossed the S129 heterozygote mutant mice with wild-type C57BL/6 mice to completely transfer the genotype from the S129 mice strain to the C57BL/6 strain. After eight backcrossings, heterozygote mice were mated to obtain homozygote auxilin knockout mice.


Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Neurobiology

Strain Origin

Donor
Lois Greene, Ph.D., National Heart, Lung, and Blood Institute (NHLBI).
Primary Reference

Yim YI, Sun T, Wu LG, Raimondi A, De Camilli P, Eisenberg E, Greene LE. Endocytosis and clathrin-uncoating defects at synapses of auxilin knockout mice. Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4412-7. doi: 10.1073/pnas.1000738107. Epub 2010 Feb 16. (Medline PMID: 20160091)

Edvardson S, Cinnamon Y, Ta-Shma A, Shaag A, Yim YI, Zenvirt S, Jalas C, Lesage S, Brice A, Taraboulos A, Kaestner KH, Greene LE, Elpeleg O. A deleterious mutation in DNAJC6 encoding the neuronal-specific clathrin-uncoating co-chaperone auxilin, is associated with juvenile parkinsonism. PLoS One. 2012;7(5):e36458. doi: 10.1371/journal.pone.0036458. Epub 2012 May 1. (Medline PMID: 22563501)

Colony and Husbandry Information

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Sib-mating
Breeding Scheme(s)

use harem breeding because some females have fertility problems

Generation
N20
Overall Breeding Performance
Poor

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Reduced Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 months 10 months
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.