Strain Name:
B6.129S7-Chrnb4tm1Mdb/Mmnc
Stock Number:
000210-UNC
Citation ID:
RRID:MMRRC_000210-UNC

Gene Information

Allele Symbol: Chrnb4tm1Mdb (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, beta polypeptide 4; targeted mutation 1, Mariella De Biasi
Synonyms: beta4 -
Chromosome: 9
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:2158716
Gene Symbol: Chrnb4 (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb-4, Acrb4
Chromosome: 9
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:87892
Genetic Alterations
a puromycin resistance cassette replaces part of intron 4 and most of exon 5
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2
    Phenotype
    These mice are indistinguishable from wild-type littermates in a cage but have autonomic cardiac and intestinal dysfunction. When combined with a b2 nAChR null mutant mouse, they develop megacystis, microcolon and hypoperistalsis.
    Mammalian Phenotype Terms
    Allelic Composition: Chrnb4tm1Mdb/Chrnb4tm1Mdb (Genetic Background: B6.129-Chrnb4tm1Mdb )

    Allelic Composition: Chrnb4tm1Mdb/Chrnb4tm1Mdb (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J )

    Strain Development
    Congenic mice with _4 subunit deficiency (_4_/_) were backcrossed eight generations onto C57BL/6J background and wild-type (WT) control mice. A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6.129S7-Chrnb4tm1Mdb/Mmnc MMRRC:000210-UNC C57BL/6J 129S7/AB2.2 2461 0 115 6
    • Cardiovascular
    • Models for Human Disease
    • Neurobiology
    Donor
    Lisa Marubio, Ph.D., Baylor College of Medicine
    Primary Reference
    Wang N, Orr-Urtreger A, Chapman J, Rabinowitz R, Korczyn AD. Deficiency of nicotinic acetylcholine receptor beta 4 subunit causes autonomic cardiac and intestinal dysfunction. Mol Pharmacol. 2003 Mar;63(3):574-80. (Medline PMID: 12606764)

    Colony and Husbandry Informationon

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Inbred
    Breeding Scheme(s)
    Homozygous female x Homozygous male
    Generation
    N11
    Overall Breeding Performance
    Normal
    Average litter size
    6 - 8
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    000210-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.