Strain Name:
C57BL/6J-Tg(HLA-A2/H2-K)1Scr/Mmnc
Stock Number:
000217-UNC
Citation ID:
RRID:MMRRC_000217-UNC

Gene Information

Transgene: H2-K (Mus musculus (mouse))
Name: histocompatibility 2, K region
Chromosome: 17
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:3040519
Transgene: Tg(HLA-A2/H2-K)1Scr (Mus musculus (mouse))
Name: transgene insertion 1, The Scripps Research Institute; transgene insertion 1, The Scripps Research Institute
Synonyms: CyHLA A2.1/Kb
Chromosome: unknown
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:3050815
Genetic Alterations
Transgenic mouse expresses genomic HLA A2.1 ( alpha 1 + alpha 2 domains) linked to Kb (alpha 3 domain and transmembrane and cytoplasmic domains)
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    N/A
    Phenotype
    Expresses HLA A2.1/Kb chimeric molecule.
    MeSH Terms
    • Amino Acid Sequence
    • Animals
    • Antigen Presentation/genetics
    • Base Sequence
    • Binding Sites/genetics
    • Binding Sites/immunology
    • Epitopes, T-Lymphocyte/genetics
    • Epitopes, T-Lymphocyte/immunology
    • Epitopes, T-Lymphocyte/physiology
    • Genetic Vectors/chemical synthesis
    • Genetic Vectors/immunology
    • HIV-1/genetics
    • HIV-1/immunology
    • HLA Antigens/genetics
    • HLA Antigens/immunology
    • Hepatitis B virus/genetics
    • Hepatitis B virus/immunology
    • Histocompatibility Antigens Class I/genetics
    • Histocompatibility Antigens Class I/immunology
    • Histocompatibility Antigens Class I/metabolism
    • Humans
    • Jurkat Cells
    • Mice
    • Mice, Transgenic
    • Molecular Sequence Data
    • Protein Sorting Signals/immunology
    • T-Lymphocytes, Cytotoxic/immunology
    • Transfection
    • Vaccines, DNA/genetics
    • Vaccines, DNA/immunology
    • Buffers
    • CD8-Positive T-Lymphocytes/immunology
    • CD8-Positive T-Lymphocytes/metabolism
    • DNA/administration & dosage
    • DNA/immunology
    • Drug Contamination
    • Emulsions
    • Epitopes, T-Lymphocyte/administration & dosage
    • Epitopes, T-Lymphocyte/chemistry
    • Freund's Adjuvant/immunology
    • Injections, Intramuscular
    • Injections, Subcutaneous
    • Interferon-gamma/biosynthesis
    • Lymphocyte Activation
    • Mice, Inbred BALB C
    • Peptide Fragments/administration & dosage
    • Peptide Fragments/chemical synthesis
    • Peptide Fragments/immunology
    • Solubility
    • T-Lymphocytes, Helper-Inducer/immunology
    • T-Lymphocytes, Helper-Inducer/metabolism
    • Transgenes/immunology
    • Vaccination/methods
    • Vaccines, Synthetic/administration & dosage
    • Vaccines, Synthetic/immunology
    • Carcinoembryonic Antigen/immunology
    • Gene Products, pol/chemical synthesis
    • Gene Products, pol/immunology
    • HLA-A2 Antigen/immunology
    • HT29 Cells
    • Hepatitis B Core Antigens/immunology
    • Interleukin-10/metabolism
    • Interleukin-5/metabolism
    • Mice, Inbred C57BL
    • Peptide Biosynthesis
    • Peptides/chemistry
    • Peptides/immunology
    • Structure-Activity Relationship
    • Tumor Cells, Cultured
    Suggested Control Mice
    • C57BL/6J
    • Cancer
    • Immunology and Inflammation
    • Models for Human Disease
    • Virology
    Donor
    Linda A. Sherman, Ph.D., The Scripps Research Institute
    Primary Reference
    • Ishioka GY, Fikes J, Hermanson G, Livingston B, Crimi C, Qin M, del Guercio MF, Oseroff C, Dahlberg C, Alexander J, Chesnut RW, Sette A. Utilization of MHC class I transgenic mice for development of minigene DNA vaccines encoding multiple HLA-restricted CTL epitopes. J Immunol. 1999 Apr 1;162(7):3915-25. (Medline PMID: 10201910)
    • Alexander J, Oseroff C, Dahlberg C, Qin M, Ishioka G, Beebe M, Fikes J, Newman M, Chesnut RW, Morton PA, Fok K, Appella E, Sette A. A decaepitope polypeptide primes for multiple CD8+ IFN-gamma and Th lymphocyte responses: evaluation of multiepitope polypeptides as a mode for vaccine delivery. J Immunol. 2002 Jun 15;168(12):6189-98. (Medline PMID: 12055232)
    • Tangri S, Ishioka GY, Huang X, Sidney J, Southwood S, Fikes J, Sette A. Structural features of peptide analogs of human histocompatibility leukocyte antigen class I epitopes that are more potent and immunogenic than wild-type peptide. J Exp Med. 2001 Sep 17;194(6):833-46. PMID: 11560998 (Medline PMID: 11560998)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color
    Black
    Breeding Scheme(s)
    Confirmed immunologic haplotype female x Confirmed immunologic haplotype male
    Generation
    Not available
    Overall Breeding Performance
    Good
    Average litter size
    5 to 6
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    000217-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.