Promoter: tetOp (Synthetic)
Name: tetracycline operon
Alteration at locus: Transgenic
Name: transgene insertion 1, Ming-Ling Chang
Alteration at locus: Transgenic
ES Cell Line: Not applicable
Homozygous phenotype:Not evaluated
Hemizygous transgenic phenotype: To mimic hepatitis C infection in humans, Tg(tetO-HCV)1Mlch hemizygotes are bred to mice expressing the Cebpb-tTA (LaptTA) transgene and maintained on doxycycline through weaning to replicate the timing of hepatitis C infection in humans. Following withdrawal of doxycycline (dox), 50 to 60% of hepatocytes express the HCV core; expression is localized to the cytoplasmic vesicle membrane. HCV-positive hepatocytes are distributed randomly within the liver lobules. Expression is not detected in any of the other tissues tested. Mice do not exhibit significant hepatic inflammation although aminotransferase levels are elevated at one month after dox withdrawal. Hepatic steatosis is observed at two months of age; initially appearing as prominent microvesicular steatosis the pattern evolves to low-level macrovesicular steatosis paralleling the reduction in HCV-core expressing hepatocytes that occurs over time. This mutant mouse strain may be useful in studies of hepatic steatosis and hepatitis C infection.
Strain of Origin: FVB/N
Strain genetic background: C57BL/6; NRMI; FVB/N
Strain Development: The tetO-HCV transgene contains sequence from hepatitis C virus (HCV) core derived from the plasma of an infected patient and cloned into a vector containing a tetracycline response element (TRE). The construct was microinjected into FVB/N fertilized ova. Founder lines were established and crossed to mice carrying the Tg(Cebpb-tTA)5Bjd (also know as LaptTA) transgene on a mixed NMRI, FVB/N and C57BL/6 background. Upon arrival, the Tg(Cebpb-tTA)5Bjd transgene was bred out of the strain.
Suggested Control Mice: Wild-ype littermates
Donor: Robert Farese, Ph.D., Gladstone Institutes
Chang ML; Chen JC; Yeh CT; Sheen IS; Tai DI; Chang MY; Chiu CT; Lin DY; Bissell DM, Topological and evolutional relationships between HCV core protein and hepatic lipid vesicles: studies in vitro and in conditionally transgenic mice., World J Gastroenterol 2007 Jul 7;13(25):3472-7 (Medline PMID: 17659694)
When maintaining a live colony, mice hemizygous for the transgene may be bred together or to wildtype siblings.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.