Strain Detail Sheet

Strain Name    :

STOCK Tg(tetO-HCV)1Mlch/Mmjax

Stock Number :

036053-JAX

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(tetO-HCV)1Mlch
Name: transgene insertion 1, Ming-Ling Chang
Alteration at locus: Transgenic
Promoter: tetO (Synthetic)
Name: tetracycline operon
Alteration at locus: Transgenic

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype:Not evaluated

Hemizygous transgenic phenotype: To mimic hepatitis C infection in humans, Tg(tetO-HCV)1Mlch hemizygotes are bred to mice expressing the Cebpb-tTA (LaptTA) transgene and maintained on doxycycline through weaning to replicate the timing of hepatitis C infection in humans. Following withdrawal of doxycycline (dox), 50 to 60% of hepatocytes express the HCV core; expression is localized to the cytoplasmic vesicle membrane. HCV-positive hepatocytes are distributed randomly within the liver lobules. Expression is not detected in any of the other tissues tested. Mice do not exhibit significant hepatic inflammation although aminotransferase levels are elevated at one month after dox withdrawal. Hepatic steatosis is observed at two months of age; initially appearing as prominent microvesicular steatosis the pattern evolves to low-level macrovesicular steatosis paralleling the reduction in HCV-core expressing hepatocytes that occurs over time. This mutant mouse strain may be useful in studies of hepatic steatosis and hepatitis C infection.

Strain of Origin: FVB/N

Strain genetic background: C57BL/6; NRMI; FVB/N

Strain Development: The tetO-HCV transgene contains sequence from hepatitis C virus (HCV) core derived from the plasma of an infected patient and cloned into a vector containing a tetracycline response element (TRE). The construct was microinjected into FVB/N fertilized ova. Founder lines were established and crossed to mice carrying the Tg(Cebpb-tTA)5Bjd (also know as LaptTA) transgene on a mixed NMRI, FVB/N and C57BL/6 background. Upon arrival, the Tg(Cebpb-tTA)5Bjd transgene was bred out of the strain.

Suggested Control Mice: Wild-ype littermates

Research Applications

  • Diabetes/Obesity
  • Metabolism
  • Virology

Strain Origin

Donor: Robert Farese, Ph.D., Gladstone Institutes

Primary Reference:

Chang ML; Chen JC; Yeh CT; Sheen IS; Tai DI; Chang MY; Chiu CT; Lin DY; Bissell DM, Topological and evolutional relationships between HCV core protein and hepatic lipid vesicles: studies in vitro and in conditionally transgenic mice., World J Gastroenterol 2007 Jul 7;13(25):3472-7 (Medline PMID: 17659694)

Colony and Husbandry Information

Special Considerations

When maintaining a live colony, mice hemizygous for the transgene may be bred together or to wildtype siblings.

Health Status Report

Colony's Current Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Order Request Information

Availability Level

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Conditions of Distribution [Including applicable technology transfer agreements]

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
036053-JAX-HEMI-FHemizygous female
036053-JAX-HEMI-MHemizygous male
036053-JAX-WT-FWild type female
036053-JAX-WT-MWild type male
$218.00
Non-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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