Strain Name:
Stock Number:
Citation ID:
Other Names:
Atp12a, colonic H,K-ATPase; non-gastric H,K-ATPase

Gene Information

Name: ATPase, H+/K+ transporting, nongastric, alpha polypeptide; targeted mutation 1, Gary E Shull
Synonyms: cHKA -
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Name: ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Synonyms: Atp1al1, cHKA, HKalpha2, ATPase H+K+-transporting, alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
NCBI: 192113
VEGA: 14
Homologene: 68197
Genetic Alterations
The Atp12a gene was disrupted in 129/SvJ ES cells by inserting the neo gene into exon 20, thus disrupting critical transmembrane domains required for ion transport. 
Genotype Determination
ES Cell Line
Not specified

Homozygous: potassium wasting in colon when subjected to a low potassium or low sodium diet; impaired absorption of sodium in the colon via the epithelial sodium channel, which is critical in diarrhea states; loss of acidification of prostate fluid.

Heterozygous: Normal

Mammalian Phenotype Terms
Allelic Composition: Atp12atm1Ges/Atp12atm1Ges (Genetic Background: involves: Black Swiss )

MeSH Terms
  • Aldosterone/blood
  • Animals
  • Body Weight
  • Colon/enzymology
  • Electrolytes/blood
  • Feces
  • Glomerular Filtration Rate
  • H(+)-K(+)-Exchanging ATPase/biosynthesis
  • H(+)-K(+)-Exchanging ATPase/deficiency
  • H(+)-K(+)-Exchanging ATPase/genetics
  • Kidney/enzymology
  • Kidney/physiology
  • Mice
  • Mice, Knockout
  • Muscle, Skeletal/metabolism
  • Organ Size
  • Potassium/metabolism
  • Potassium/urine
  • RNA, Messenger/biosynthesis
  • Sodium/urine
  • Amiloride/pharmacology
  • Blotting, Western
  • Diet, Sodium-Restricted
  • Electric Conductivity
  • Epithelium/chemistry
  • Epithelium/physiology
  • Feces/chemistry
  • Genotype
  • H(+)-K(+)-Exchanging ATPase/physiology
  • Intestinal Absorption
  • Mice, Inbred C57BL
  • Polymerase Chain Reaction
  • Potassium/blood
  • RNA, Messenger/analysis
  • Sodium/blood
  • Sodium/metabolism
  • Sodium Channels/genetics
  • H(+)-K(+)-Exchanging ATPase/chemistry
  • H(+)-K(+)-Exchanging ATPase/metabolism
  • Hydrogen-Ion Concentration
  • Male
  • Mitochondrial Proton-Translocating ATPases/metabolism
  • Molecular Chaperones/metabolism
  • Prostate/metabolism
  • Proton Pumps/chemistry
  • Proton Pumps/metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Stomach/enzymology
Strain Development
Corrrectly targeted ES cells were intected into C57BL/6J blastocysts. Chimeric males bred with Black Swiss females yielded an animal carrying the disrupted cHKA gene in its germline. This mouse was bred to establish a colony on a NSA (Non Swiss Albino) background. This STOCK was further bred to BALB/c mice backcrossing an additional 10 generations making it congenic on the BALB/c background.
Suggested Control Mice
Wild-type littermates or BALB/c
  • Cancer
  • Internal Organ
  • Other
Gary Shull, Ph.D., University of Cincinnati College of Medicine.
Primary Reference

Meneton P, Schultheis PJ, Greeb J, Nieman ML, Liu LH, Clarke LL, Duffy JJ, Doetschman T, Lorenz JN, Shull GE. Increased sensitivity to K+ deprivation in colonic H,K-ATPase-deficient mice. J Clin Invest. 1998 Feb 1;101(3):536-42. (Medline PMID:9449685).

Spicer Z, Clarke LL, Gawenis LR, Shull GE. Colonic H(+)-K(+)-ATPase in K(+) conservation and electrogenic Na(+) absorption during Na(+) restriction. Am J Physiol Gastrointest Liver Physiol. 2001 Dec;281(6):G1369-77. (Medline PMID:11705741).

Pestov NB, Korneenko TV, Shakhparonov MI, Shull GE, Modyanov NN. Loss of acidification of anterior prostate fluids in Atp12a-null mutant mice indicates that nongastric H-K-ATPase functions as proton pump in vivo. Am J Physiol Cell Physiol. 2006 Aug;291(2):C366-74. (Medline PMID:16525125).

Colony and Husbandry Information

no special requirements; mice appear healthy
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
Coat Color
MMRRC Breeding System
Breeding Scheme(s)

BALB/c female x Heterozygous male or reciprocal mating

At least 10
Overall Breeding Performance
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036713-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
036713-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.