Strain Name:
STOCK Khdc3tm1Dean/Mmnc
Stock Number:
032880-UNC
Citation ID:
RRID:MMRRC_032880-UNC

Gene Information

Allele Symbol: Khdc3tm1Dean [MGI:3844604] (Mus musculus (mouse))
Name: KH domain containing 3, subcortical maternal complex member; targeted mutation 1, Jurrien Dean
Synonyms: Filiatm
Chromosome: 9
Alteration at locus: Knockout
Gene Symbol: Khdc3 [MGI:1914241] (Mus musculus (mouse))
Name: KH domain containing 3, subcortical maternal complex member
Synonyms: ecat1, 2410004A20Rik, FILIA
Chromosome: 9
Alteration at locus: Knockout
Genetic Alterations
Neo cassette replaced transcription and translational start sites as well as exons 1 and 2 of the coding region.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype
    Homozygous: Reduced fecundity due to pre-implantation defects.

    Heterozygous: Normal fecundity.
    Mammalian Phenotype Terms
    Allelic Composition: Khdc3tm1Dean/Khdc3tm1Dean (Genetic Background: involves: 129/Sv * C57BL/6 )

    MeSH Terms
    • Animals
    • Aurora Kinase A
    • Aurora Kinases
    • Cell Cycle Proteins/metabolism
    • Cleavage Stage, Ovum/cytology
    • Cleavage Stage, Ovum/physiology
    • Embryo, Mammalian/cytology
    • Embryo, Mammalian/metabolism
    • Embryo, Mammalian/physiology
    • Embryonic Development
    • Female
    • Mad2 Proteins
    • Mice
    • Mice, Mutant Strains
    • Ploidies
    • Protein-Serine-Threonine Kinases/metabolism
    • Proteins/genetics
    • Proteins/physiology
    • Proto-Oncogene Proteins/metabolism
    • Spindle Apparatus/metabolism
    • Tubulin/metabolism
    Strain Development
    KO made with 129 ES cells and B6 blastocyst; bred to FVBs to maintain colony.
    Suggested Control Mice
    • Wildtype littermates
    • Developmental Biology
    • Reproduction
    • embryology
    Donor
    Jurrien Dean, M.D., Ph.D, National Institute of Diabetes and Digestive and Kidney Diseases/National Institutes of Health
    Primary Reference
    Zheng P; Dean J, Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis., Proc Natl Acad Sci U S A 2009 May 5;106(18):7473-8. 19376971 (Medline PMID: 19376971)

    Colony and Husbandry Information

    Random intra-strain mating. Breeder chow for breeding cages; breeder chow one week for weanlings.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    032880-UNC-SPERM Cryo-preserved spermatozoa $548.00 / $910.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2
    032880-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / $4,696.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.