Strain Detail Sheet

Strain Name    :

STOCK Khdc3tm1Dean/Mmnc

Stock Number :

032880-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Khdc3tm1Dean
Name: targeted mutation 1, Jurrien Dean
Alteration at locus: Knockout
Gene Symbol: Khdc3
Name: KH domain containing 3, subcortical maternal complex member
Chromosome: 9
Alteration at locus: Knockout

Genetic Alterations:
Neo cassette replaced transcription and translational start sites as well as exons 1 and 2 of the coding region.

Genotype Determination:

ES Cell Line: Not specified derived from B6;129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Reduced fecundity due to pre-implantation defects.

Heterozygous phenotype: Normal fecundity.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
2410004A20Riktm1Dean/2410004A20Riktm1Dean
        involves: 129/Sv * C57BL/6
  • other phenotype
    • maternal effect (MGI Ref ID J:148330)
      • significantly fewer embryos reach the morula or blastocyst stage when the mother is homozygous null
      • when the mother is homozygous null, embryonic development is delayed in some embryos as a result of a prolongation of the G2 phase of cell division and many of these delayed embryos display aneuploidy
      • delayed embryos display spindle abnormalities and defects in spindle assembly checkpoint function
  • reproductive system phenotype
    • reproductive system phenotype (MGI Ref ID J:148330)
      • no defects in oogenesis, ovulation or fertilization are detected and the subcortical maternal complex persists
      • decreased litter size (MGI Ref ID J:148330)
        • litter size is reduced when females are crossed to homozygous, heterozygous, or wild-type males
      • reduced female fertility (MGI Ref ID J:148330)
        • during a 6 month span females produced from 0 to 5 litters and the time from mating to delivery was increased to about 30 days (range 20 - 120 days) cmpared to about 20 days in controls

Strain of Origin: 129, B6 mix

Strain genetic background: 129, B6, FVB mix

Strain Development: KO made with 129 ES cells and B6 blastocyst; bred to FVBs to maintain colony.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Reproduction
  • embryology

Strain Origin

Donor: Jurrien Dean, MD, PhD, NIDDK/NIH

Primary Reference:

Zheng P; Dean J, Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis., Proc Natl Acad Sci U S A 2009 May 5;106(18):7473-8. 19376971 (Medline PMID: 19376971)

Colony and Husbandry Information

Special Considerations

Random intra-strain mating. Breeder chow for breeding cages; breeder chow one week for weanlings.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
032880-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
032880-UNC-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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