Strain Name:
B6.129S6-Kcnk2tm1Lex/Mmucd
Stock Number:
036938-UCD
Citation ID:
RRID:MMRRC_036938-UCD
Other Names:
KCNK2 -/-, TREK-1 knockout

Strain Information

Kcnk2tm1Lex
Name: potassium channel, subfamily K, member 2; targeted mutation 1, Lexicon Genetics
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Kcnk2
Name: potassium channel, subfamily K, member 2
Synonyms: TREK-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 16526
HGNC: HGNC:6277
Homologene: 7794
Genetic Alterations
Exon2, intron2, exon3, and the first 23 bp of the intron3 for Kcnk2 gene were replaced by a β-galactosidase/neomycin selection cassette.
Genotype Determination
ES Cell Line
Not specified
Phenotype

Homozygous: Mice show altered electrical communication in the retina, more susceptable to lung injury.

Heterozygous: Not characterized.

Mammalian Phenotype Terms
Allelic Composition: Kcnk2tm1Lex/Kcnk2tm1Lex (Genetic Background: involves: 129 * C57BL/6 )

MeSH Terms
  • Action Potentials
  • Animals
  • Aorta/metabolism
  • Arachidonic Acid/metabolism
  • Basilar Artery/drug effects
  • Basilar Artery/metabolism
  • Cerebrovascular Circulation/drug effects
  • Dose-Response Relationship, Drug
  • Genotype
  • Large-Conductance Calcium-Activated Potassium Channels/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Middle Cerebral Artery/drug effects
  • Middle Cerebral Artery/metabolism
  • Muscle, Smooth, Vascular/metabolism
  • Myocytes, Smooth Muscle/metabolism
  • Nitric Oxide/metabolism
  • Phenotype
  • Potassium/metabolism
  • Potassium Channel Blockers/pharmacology
  • Potassium Channels, Tandem Pore Domain/deficiency
  • Potassium Channels, Tandem Pore Domain/genetics
  • Prostaglandin-Endoperoxide Synthases/metabolism
  • Vasoconstriction
  • Vasoconstrictor Agents/pharmacology
  • Vasodilation/drug effects
  • Vasodilator Agents/pharmacology
  • alpha-Linolenic Acid/metabolism
  • Brain Injuries/metabolism
  • Brain Injuries/pathology
  • Brain Injuries/physiopathology
  • Cell Count
  • Cell Survival
  • Cerebral Cortex/blood supply
  • Hippocampus/pathology
  • Hippocampus/physiopathology
  • Laser-Doppler Flowmetry
  • Male
  • Neurons/pathology
  • Neuroprotective Agents/metabolism
  • Potassium Channels, Tandem Pore Domain/metabolism
  • Regional Blood Flow
  • Amacrine Cells/drug effects
  • Amacrine Cells/metabolism
  • Amacrine Cells/physiology
  • Arachidonic Acid/pharmacology
  • Calcium/metabolism
  • Membrane Potentials
  • Transcription, Genetic
Strain Development
The targeting vector was electroporated into the ES cells derived from the 129S6/SvEvTac. The selected ES cells were introduced into a blastocyst and implanted into a psedopregnant mouse to create a chimeric mouse. The chimeric mouse was bred to C57BL/6 mouse and backcrossed to C57BL/6 for 10 generations.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
    • Depression
    • Brain Injury (neuroprotection)
  • Neurobiology
  • Sensorineural
Donor
Robert Bryan, Ph.D., Baylor College of Medicine.
Primary Reference

Namiranian K, Lloyd EE, Crossland RF, Marrelli SP, Taffet GE, Reddy AK, Hartley CJ and Bryan RM, Jr. Cerebrovascular responses in mice deficient in the potassium channel, TREK-1. Am J Physiol Regul Integr Comp Physiol. 2010 Aug;299(2):R461-9. doi: 10.1152/ajpregu.00057.2010. Epub 2010 Mar 31. (Medline PMID:20357027)

Namiranian K, Brink CD, Goodman JC, Robertson CS and Bryan RM, Jr. Traumatic brain injury in mice lacking the K channel, TREK-1. J Cereb Blood Flow Metab. 2011 Mar;31(3):e1-6. doi: 10.1038/jcbfm.2010.223. Epub 2010 Dec 15. (Medline PMID:21157470)

Ford KJ, Arroyo D, Kay J, Lloyd EE, Bryan RM, Jr., Sanes J and Feller MB. A role for TREK1 in generating the slow afterhyperpolarization in developing starburst amacrine cells. J Neurophysiol. 2013 May;109(9):2250-9. doi: 10.1152/jn.01085.2012. Epub 2013 Feb 6. (Medline PMID:23390312)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)

Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating

Generation
N10+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 12 months 12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036938-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
036938-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
036938-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.