Strain Name:
Stock Number:
Citation ID:
Other Names:
KCNK2 -/-, TREK-1 knockout

Gene Information

Allele Symbol: Kcnk2tm1Lex (Mus musculus (mouse))
Name: potassium channel, subfamily K, member 2; targeted mutation 1, Lexicon Genetics
Chromosome: 1
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:3528830
Gene Symbol: Kcnk2 (Mus musculus (mouse))
Name: potassium channel, subfamily K, member 2
Synonyms: TREK-1
Chromosome: 1
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:109366
Genetic Alterations
Exon2, intron2, exon3, and the first 23 bp of the intron3 for Kcnk2 gene were replaced by a β-galactosidase/neomycin selection cassette.
Genotype Determination
ES Cell Line
Not specified

Homozygous: Mice show altered electrical communication in the retina, more susceptable to lung injury.

Heterozygous: Not characterized.

Mammalian Phenotype Terms
Allelic Composition: Kcnk2tm1Lex/Kcnk2tm1Lex (Genetic Background: involves: 129 * C57BL/6 )

Strain Development
The targeting vector was electroporated into the ES cells derived from the 129S6/SvEvTac. The selected ES cells were introduced into a blastocyst and implanted into a psedopregnant mouse to create a chimeric mouse. The chimeric mouse was bred to C57BL/6 mouse and backcrossed to C57BL/6 for 10 generations.
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
    • Depression
    • Brain Injury (neuroprotection)
  • Neurobiology
  • Sensorineural
Robert Bryan, Ph.D., Baylor College of Medicine.
Primary Reference
  • Namiranian K, Lloyd EE, Crossland RF, Marrelli SP, Taffet GE, Reddy AK, Hartley CJ and Bryan RM, Jr. Cerebrovascular responses in mice deficient in the potassium channel, TREK-1. Am J Physiol Regul Integr Comp Physiol. 2010 Aug;299(2):R461-9. doi: 10.1152/ajpregu.00057.2010. Epub 2010 Mar 31. (Medline PMID:20357027)
  • Namiranian K, Brink CD, Goodman JC, Robertson CS and Bryan RM, Jr. Traumatic brain injury in mice lacking the K channel, TREK-1. J Cereb Blood Flow Metab. 2011 Mar;31(3):e1-6. doi: 10.1038/jcbfm.2010.223. Epub 2010 Dec 15. (Medline PMID:21157470)
  • Ford KJ, Arroyo D, Kay J, Lloyd EE, Bryan RM, Jr., Sanes J and Feller MB. A role for TREK1 in generating the slow afterhyperpolarization in developing starburst amacrine cells. J Neurophysiol. 2013 May;109(9):2250-9. doi: 10.1152/jn.01085.2012. Epub 2013 Feb 6. (Medline PMID:23390312)

Colony and Husbandry Informationon

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
Coat Color
MMRRC Breeding System
Breeding Scheme(s)
  • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
Overall Breeding Performance
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 12 months 12 months
Average litter size
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036938-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
036938-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
036938-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.