Name: transgene insertion 1, A Francis Stewart
Alteration at locus: Transgenic
ES Cell Line: Not Applicable
Both homozygous and hemizygous mice express Flpo recombinase under the CAG promotor.
Strain of Origin: C57BL/6N
Strain genetic background: C57BL/6N
Strain Development: This strain was developed by backcrossing C57BL/6-Tg(CAG-Flpo)1Afst/Mmcd, (MMRRC:032247) onto C57BL/6NTac. Originally, correctly targeted mutant cells were injected into 8-cell C57BL/6J albino embryos. Chimeras were mated to C57BL/6J albino mice and pups were screened by Southern Blot for germline transmission. Germline heterozygotes were mated to C57BL/6JOlaHsd mice. Mice were rederived with C57BL/6J and are maintained with random intra-strain mating. Upon receipt, the MMRRC backcrossed to C57BL/6NTac.
Suggested Control Mice: Wild-type littermates
Donor: MMRRC at UCD, University of California, Davis. The original transgenic was donated by Dr. Konstantinos Anastassiadis from Technische Universitaet Dresden.
Kranz A, Fu J, Duerschke K, Weidlich S, Naumann R, Stewart AF, Anastassiadis K. An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase. Genesis. 2010 Aug;48(8):512-20. (Medline PMID: 20506501).
Maintaining this strain on C57BL/6N ensures Nnt allele is present in full (an absence of exons 7 – 11 is associated with impaired glucose homeostasis control and reduced insulin secretion, which is a mutation found in C57BL/6J mice).
Coat color: Black
MMRRC Breeding System: Backcross or Sib-mating
Generation: N6F1 (as of January, 2012). Strain was determined to be 99.87% congenic on C57BL/6NTac by SNP analysis.
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Hetero/Hemizygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size: 6
Recommended wean age: 3 weeks
Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at email@example.com for more details.
Fee/unit (US $)
|Per Mouse||The firstname.lastname@example.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.