Strain Name:
B6N(B6J)-Tg(CAG-Flpo)1Afst/Mmucd
Stock Number:
036512-UCD
Citation ID:
RRID:MMRRC_036512-UCD
Other Names:
CAGG's-Flpo

Gene Information

CAG
Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor
Type: DNA Segment
Species: Multi-species
flpo
Name: mouse codon-optimized FLP recombinase, FLPo
Type: Gene
Species: Mus musculus (mouse)
Tg(CAG-flpo)1Afst
Name: transgene insertion 1, A Francis Stewart
Synonyms: CAGGs-Flpo, Flpo
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations

Random insertion of CAGGs promoter driven, codon-optimized Flp recombinase (Flpo) and an IRES-puromycin sequence.

ES Cell Line
JM8.F6 derived from C57BL/6N
Phenotype
Both homozygous and hemizygous mice express Flpo recombinase under the CAG promotor.
MeSH Terms
  • Animals
  • Base Sequence
  • Binding Sites/genetics
  • Cell Line
  • Cells, Cultured
  • DNA Nucleotidyltransferases/genetics
  • DNA Nucleotidyltransferases/metabolism
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/metabolism
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Female
  • Fibroblasts/cytology
  • Fibroblasts/metabolism
  • Gene Expression Regulation, Developmental
  • Gene Knockout Techniques
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Isoenzymes/genetics
  • Isoenzymes/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Plasmids/genetics
  • Recombination, Genetic
  • Transfection
Strain Development

This strain was developed by backcrossing B6J;B6N-Tg(CAG-Flpo)1Afst/Mmcd (MMRRC:32247) onto C57BL/6NTac. Correctly targeted ES cells (JM8.F6 derived from C57BL/6N) were injected into 8-cell C57BL/6J albino embryos. Chimeras were mated to C57BL/6J albino mice and pups were screened by Southern Blot for germline transmission. Germline heterozygotes were mated to C57BL/6JOlaHsd mice. Mice were rederived with C57BL/6J and are maintained with random intra-strain mating. Upon receipt, the MMRRC backcrossed to C57BL/6NTac to the sixth generation.

Suggested Control Mice
Wild-type littermates
  • Models for Human Disease
  • Research Tools
Donor
Konstantinos Anastassiadis, Ph.D., Technische Universitaet Dresden.
MMRRC at UCD, University of California, Davis.
Francis Stewart, Ph.D., Technische Universitaet Dresden.
Primary Reference
Kranz A, Fu J, Duerschke K, Weidlich S, Naumann R, Stewart AF, Anastassiadis K. An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase. Genesis. 2010 Aug;48(8):512-20. (Medline PMID: 20506501).

Colony and Husbandry Information

Maintaining this strain on C57BL/6N ensures that the Nnt allele is present in full (an absence of exons 7 – 11 is associated with impaired glucose homeostasis control and reduced insulin secretion, which is a mutation found in C57BL/6J mice).

Colony's Current Health Status Report

For more information about this colony's health status contact mmrrc@ucdavis.edu
Coat Color
Black
MMRRC Breeding System
Backcross and sib-mating
Breeding Scheme(s)
  • C57BL/6N female x Hemizygous male or reciprocal
  • Hemizygous female x Hemizygous male
Generation
N6F1 (as of January 2012). The strain was determined to be 99.87% congenic on C57BL/6NTac by SNP analysis.
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@ucdavis.edu for more details.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036512-UCD-HET-F
036512-UCD-HET-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
$272.50 / Non-Profit Per Mouse The mmrrc@ucdavis.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
036512-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
036512-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.