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Gene Information

Transgene: Tg(CAG-flpo)1Afst (Mus musculus (mouse))
Name: transgene insertion 1, A Francis Stewart; transgene insertion 1, A Francis Stewart
Synonyms: CAGGs-Flpo, Flpo
Chromosome: unknown
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:4453967
Genetic Alterations
JM8 ES cells were electroporated with linearized construct CAGGs-FLPo-IRES-puro.
Genotype Determination
ES Cell Line
Both homozygous and hemizygous mice express Flpo recombinase under the CAG promotor.
Strain Development
This strain was developed by backcrossing C57BL/6-Tg(CAG-Flpo)1Afst/Mmcd, (MMRRC:032247) onto C57BL/6NTac. Originally, correctly targeted mutant cells were injected into 8-cell C57BL/6J albino embryos. Chimeras were mated to C57BL/6J albino mice and pups were screened by Southern Blot for germline transmission. Germline heterozygotes were mated to C57BL/6JOlaHsd mice. Mice were rederived with C57BL/6J and are maintained with random intra-strain mating. Upon receipt, the MMRRC backcrossed to C57BL/6NTac.
Suggested Control Mice
Wild-type littermates
  • Models for Human Disease
  • Research Tools
MMRRC at UCD, University of California, Davis.
Primary Reference
Kranz A, Fu J, Duerschke K, Weidlich S, Naumann R, Stewart AF, Anastassiadis K. An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase. Genesis. 2010 Aug;48(8):512-20. (Medline PMID: 20506501).

Colony and Husbandry Informationon

Maintaining this strain on C57BL/6N ensures Nnt allele is present in full (an absence of exons 7 – 11 is associated with impaired glucose homeostasis control and reduced insulin secretion, which is a mutation found in C57BL/6J mice).


Colony's Current Health Status Report

For more information about this colony's health status contact
Coat Color
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • C57Bl/6N female x Hemizygous male or reciprocal
  • Hemizygous female x Hemizygous male
N6F1 (as of January, 2012).  Strain was determined to be 99.87% congenic on C57BL/6NTac by SNP analysis.
Overall Breeding Performance
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at for more details.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
$272.50 / Non-Profit Per Mouse The may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
036512-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
036512-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.