Name: transgene insertion 1, A Francis Stewart
Alteration at locus: Transgenic
ES Cell Line: Not Applicable
Mice express Flpo recombinase under the CAGGs promotor
Strain of Origin: C57BL/6N
Strain genetic background: C57BL/6
Strain Development: Correctly targeted mutant cells were injected into 8-cell C57BL/6J albino embryos. Chimeras were mated to C57BL/6J albino mice and pups were screened by Southern Blot for germline transmission. Germline heterozygotes were mated to C57BL/6JOlaHsd mice. Mice were rederived with C57BL/6J and are maintained with random intra-strain mating.
Suggested Control Mice: Wild-type littermates
Donor: Konstantinos Anastassiadis, Ph.D., Technische Universitaet Dresden
Kranz A, Fu J, Duerschke K, Weidlich S, Naumann R, Stewart AF, Anastassiadis K. An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase. Genesis. 2010 Aug;48(8):512-20. (Medline PMID: 20506501)
Coat color: Black
MMRRC Breeding System: Backcross or Sib-mated
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Hom/Hemizygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes|
|Het/Hemizygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size: 6
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.