Strain Detail Sheet

Strain Name    :

C57BL/6-Tg(CAG-Flpo)1Afst/Mmucd

Stock Number :

032247-UCD

Other Names   :

CAGG's-Flpo, C57BL/6-Tg(CAG-Flpo)1Afst/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(CAG-Flpo)1Afst
Name: transgene insertion 1, A Francis Stewart
Alteration at locus: Transgenic

Genetic Alterations:
JM8 ES cells were electroporated with linearized construct CAGGs-FLPo-IRES-puro.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Mice express Flpo recombinase under the CAGGs promotor

Strain of Origin: C57BL/6N

Strain genetic background: C57BL/6

Strain Development: Correctly targeted mutant cells were injected into 8-cell C57BL/6J albino embryos. Chimeras were mated to C57BL/6J albino mice and pups were screened by Southern Blot for germline transmission. Germline heterozygotes were mated to C57BL/6JOlaHsd mice. Mice were rederived with C57BL/6J and are maintained with random intra-strain mating.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: Konstantinos Anastassiadis, Ph.D., Technische Universitaet Dresden

Primary Reference:

Kranz A, Fu J, Duerschke K, Weidlich S, Naumann R, Stewart AF, Anastassiadis K. An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase. Genesis. 2010 Aug;48(8):512-20. (Medline PMID: 20506501)

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross or Sib-mated

Breeding Scheme(s):

  • C57Bl/6J female x Hemizygous male or reciprocal
  • Wild-type female x Hemizygous male sibling or reciprocal

Generation: Unknown

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Hom/Hemizygotes are viable: Yes Yes
Homozygotes are fertile: Yes  
Het/Hemizygotes are fertile: Yes Yes
 
Age Reproductive Decline: Unknown Unknown

Average litter size: 6

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
032247-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
032247-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
032247-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 04/23/2014