Strain Detail Sheet

Strain Name    :

129S/SvEv-Bex1Gt(OST125186)Lex/Mmnc

Stock Number :

036899-UNC

Other Names   :

Bex1-KO, Rex-3

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Bex1Gt(OST125186)Lex
Name: gene trap OST125186, Lexicon Genetics
Chromosome: X
Alteration at locus: Gene Trap
Gene Symbol: Bex1
Name: brain expressed gene 1
Chromosome: X
Alteration at locus: Gene Trap

Genetic Alterations:
The gene trap vector, OST125186, inserted into intron 2 of Bex1.

Genotype Determination:

    ES Cell Line: Lex-1 derived from 129S5/SvEvBrd

    Strain Description [Including phenotype, strain background, strain development and suggested control mice]

    Phenotype

    Homozygous: Anomalous olfactory transduction, muscle and axonal regeneration deficits.

    Hemizygous: Bex-1 is X-linked so males act as homozygotes.

    Strain of Origin: 129S5/SvEvBrd

    Strain genetic background: 129S6/SvEvTac

    Strain Development: Lex-1 ES cells (Clone no. OST125186, OmniBank Library, Lexicon Genetics), derived from 129S5/SvEvBrd by the gene trap method, were microinjected into blastocyst. The chimeric mice were bred for several generations to 129S6/SvEvTac mice and then bred to homozygosity. These mice were backcrossed to 129S6/SvEvTac mice.

    Suggested Control Mice: Wild-type littermates

    Research Applications

    • Cancer
    • Cell Biology
    • Models for Human Disease
      • muscle dystrophy
    • Neurobiology
    • Research Tools
    • Sensorineural

    Strain Origin

    Donor: Frank Margolis, Ph.D., University of Maryland, Baltimore, School of Medicine.

    Primary Reference:

    • Koo JH, Smiley MA, Lovering RM, Margolis FL. Bex1 knock out mice show altered skeletal muscle regeneration. Biochem Biophys Res Commun. 2007 Nov 16;363(2):405-10. PMID: 17884015
    • Khazaei MR, Halfter H, Karimzadeh F, Koo JH, Margolis FL, Young P. Bex1 is involved in the regeneration of axons after injury. J Neurochem. 2010 Nov;115(4):910-20. PMID: 20731761
    • Bernstein SL, Koo JH, Slater BJ, Guo Y, Margolis FL. Analysis of optic nerve stroke by retinal Bex expression. Mol Vis. 2006 Mar 1;12:147-55. PMID:16541015
    • JH Koo. Bex1 Participates in Muscle Regeneration by Regulating Myogenic Satellite Cell Differentiation. Lab. Anim. Res. 2010: 26(2), 145-151
    • Lee CH, Wong TS, Chan JY, Lu SC, Lin P, Cheng AJ, Chen YJ, Chang JS, Hsiao SH, Leu YW, Li CI, Hsiao JR, Chang JY. Epigenetic Regulation of the X-Linked Tumor Suppressors BEX1 and LDOC1 in Oral Squamous Cell Carcinoma. J Pathol. 2013 Jan 29. PMID:23362108
    • de Ronde JJ, Lips EH, Mulder L, Vincent AD, Wesseling J, Nieuwland M, Kerkhoven R, Vrancken Peeters MJ, Sonke GS, Rodenhuis S, Wessels LF. SERPINA6, BEX1, AGTR1, SLC26A3, and LAPTM4B are markers of resistance to neoadjuvant chemotherapy in HER2-negative breast cancer. Breast Cancer Res Treat. 2013 Jan;137(1):213-23. PMID: 23203637
    • Ding K, Su Y, Pang L, Lu Q, Wang Z, Zhang S, Zheng S, Mao J, Zhu Y. Inhibition of apoptosis by downregulation of hBex1, a novel mechanism, contributes to the chemoresistance of Bcr/Abl+ leukemic cells. Carcinogenesis. 2009 Jan;30(1):35-42. PMID: 19028701
    • Zambrowicz BP, Friedrich GA, Buxton EC, Lilleberg SL, Person C, Sands AT. Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells. Nature 1998 392;608–611. PMID: 9560157

    Colony and Husbandry Information

    Special Considerations

    None

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat color: Agouti

    Other:

    Breeding

    MMRRC Breeding System: Random intra-strain mating, Gene is X-linked

    Breeding Scheme(s):

    • Homozygous female x Hemizygous male from within the colony
    • Heterozygous female x Hemizygous male from within the colony

    Generation:

    Overall Breeding Performance: Excellent

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

    Viability and Fertility:FemaleMale
    Homozygotes are viable: Yes No Gene is X-linked
    Homozygotes are fertile: Yes No
    Hetero/Hemizygotes are fertile: Yes Yes

    Age Reproductive Decline: 10 months 10 months

    Average litter size: 5-9

    Recommended wean age: 3-4 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    036899-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    036899-UNC-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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    Generated: 03/02/2015