Strain Name:
129S/SvEv-Bex1Gt(OST125186)Lex/Mmnc
Stock Number:
036899-UNC
Citation ID:
RRID:MMRRC_036899-UNC
Other Names:
Bex1-KO, Rex-3

Gene Information

Allele Symbol: Bex1Gt(OST125186)Lex (Mus musculus (mouse))
Name: brain expressed X-linked 1; gene trap OST125186, Lexicon Genetics
Synonyms: Bex1-KO
Chromosome: X
Alteration at locus: Gene Trap
Monarch Initiative (Disease and Phenotype Associations): MGI:3764914
Gene Symbol: Bex1 (Mus musculus (mouse))
Name: brain expressed X-linked 1
Synonyms: Rex3
Chromosome: X
Alteration at locus: Gene Trap
Monarch Initiative (Disease and Phenotype Associations): MGI:1328321
Genetic Alterations
The gene trap vector, OST125186, inserted into intron 2 of Bex1.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Lex-1 derived from 129S5/SvEvBrd
    Phenotype

    Homozygous: Anomalous olfactory transduction, muscle and axonal regeneration deficits.

    Hemizygous: Bex-1 is X-linked so males act as homozygotes.

    Mammalian Phenotype Terms
    Allelic Composition: Bex1Gt(OST125186)Lex/Bex1Gt(OST125186)Lex (Genetic Background: involves: 129S5/SvEvBrd )

    MeSH Terms
    • Animals
    • Male
    • Mice
    • Mice, Knockout
    • Muscle Fatigue/physiology
    • Muscle, Skeletal/physiology
    • Nerve Tissue Proteins/physiology
    • Physical Exertion/physiology
    • Regeneration/physiology
    • Axons/physiology
    • Cells, Cultured
    • Mice, Inbred C57BL
    • Motor Neurons/physiology
    • Myelin-Associated Glycoprotein/antagonists & inhibitors
    • Myelin-Associated Glycoprotein/physiology
    • Nerve Regeneration/physiology
    • Nerve Tissue Proteins/biosynthesis
    • Up-Regulation/physiology
    • Antineoplastic Combined Chemotherapy Protocols/therapeutic use
    • Biomarkers, Tumor/genetics
    • Biomarkers, Tumor/metabolism
    • Breast Neoplasms/drug therapy
    • Breast Neoplasms/metabolism
    • Capecitabine
    • Chemotherapy, Adjuvant
    • Chloride-Bicarbonate Antiporters/genetics
    • Chloride-Bicarbonate Antiporters/metabolism
    • Cyclophosphamide/administration & dosage
    • Deoxycytidine/administration & dosage
    • Deoxycytidine/analogs & derivatives
    • Doxorubicin/administration & dosage
    • Drug Resistance, Neoplasm
    • Female
    • Fluorouracil/administration & dosage
    • Fluorouracil/analogs & derivatives
    • Gene Expression
    • Humans
    • Membrane Proteins/genetics
    • Membrane Proteins/metabolism
    • Neoadjuvant Therapy
    • Nerve Tissue Proteins/genetics
    • Nerve Tissue Proteins/metabolism
    • Oncogene Proteins/genetics
    • Oncogene Proteins/metabolism
    • RNA, Messenger/genetics
    • RNA, Messenger/metabolism
    • Receptor, Angiotensin, Type 1/genetics
    • Receptor, Angiotensin, Type 1/metabolism
    • Receptor, ErbB-2/metabolism
    • Taxoids/administration & dosage
    • Transcortin/genetics
    • Transcortin/metabolism
    • Treatment Outcome
    • Apoptosis/physiology
    • Cell Line, Tumor
    • Down-Regulation
    • Gene Silencing
    • Genes, abl
    • Leukemia/genetics
    • Leukemia/pathology
    • Leukemia/physiopathology
    • RNA, Small Interfering
    • Cell Line
    • Drug Resistance/genetics
    • Gene Library
    • Genetic Techniques
    • Genetic Vectors
    • Hypoxanthine Phosphoribosyltransferase/genetics
    • Molecular Sequence Data
    • Mutagenesis, Insertional
    • Puromycin/pharmacology
    • Sequence Analysis, DNA
    • Stem Cells
    Strain Development
    Lex-1 ES cells (Clone no. OST125186, OmniBank Library, Lexicon Genetics), derived from 129S5/SvEvBrd by the gene trap method, were microinjected into blastocyst. The chimeric mice were bred for several generations to 129S6/SvEvTac mice and then bred to homozygosity. These mice were backcrossed to 129S6/SvEvTac mice.
    Suggested Control Mice
    Wild-type littermates
    • Cancer
    • Cell Biology
    • Models for Human Disease
      • muscle dystrophy
    • Neurobiology
    • Research Tools
    • Sensorineural
    Donor
    Frank Margolis, Ph.D., University of Maryland, Baltimore, School of Medicine.
    Primary Reference
    • Koo JH, Smiley MA, Lovering RM, Margolis FL. Bex1 knock out mice show altered skeletal muscle regeneration. Biochem Biophys Res Commun. 2007 Nov 16;363(2):405-10. (Medline PMID: 17884015)
    • Khazaei MR, Halfter H, Karimzadeh F, Koo JH, Margolis FL, Young P. Bex1 is involved in the regeneration of axons after injury. J Neurochem. 2010 Nov;115(4):910-20. (Medline PMID: 20731761)
    • Bernstein SL, Koo JH, Slater BJ, Guo Y, Margolis FL. Analysis of optic nerve stroke by retinal Bex expression. Mol Vis. 2006 Mar 1;12:147-55. (Medline PMID:16541015)
    • JH Koo. Bex1 Participates in Muscle Regeneration by Regulating Myogenic Satellite Cell Differentiation. Lab. Anim. Res. 2010: 26(2), 145-151
    • Lee CH, Wong TS, Chan JY, Lu SC, Lin P, Cheng AJ, Chen YJ, Chang JS, Hsiao SH, Leu YW, Li CI, Hsiao JR, Chang JY. Epigenetic Regulation of the X-Linked Tumor Suppressors BEX1 and LDOC1 in Oral Squamous Cell Carcinoma. J Pathol. 2013 Jan 29. (Medline PMID:23362108)
    • de Ronde JJ, Lips EH, Mulder L, Vincent AD, Wesseling J, Nieuwland M, Kerkhoven R, Vrancken Peeters MJ, Sonke GS, Rodenhuis S, Wessels LF. SERPINA6, BEX1, AGTR1, SLC26A3, and LAPTM4B are markers of resistance to neoadjuvant chemotherapy in HER2-negative breast cancer. Breast Cancer Res Treat. 2013 Jan;137(1):213-23. (Medline PMID: 23203637)
    • Ding K, Su Y, Pang L, Lu Q, Wang Z, Zhang S, Zheng S, Mao J, Zhu Y. Inhibition of apoptosis by downregulation of hBex1, a novel mechanism, contributes to the chemoresistance of Bcr/Abl+ leukemic cells. Carcinogenesis. 2009 Jan;30(1):35-42. (Medline PMID: 19028701)
    • Zambrowicz BP, Friedrich GA, Buxton EC, Lilleberg SL, Person C, Sands AT. Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells. Nature. 1998 Apr 9;392(6676):608-11. (Medline PMID: 9560157)

    Colony and Husbandry Informationon

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color
    Agouti
    MMRRC Breeding System
    Random intra-strain mating, Gene is X-linked
    Breeding Scheme(s)
    • Homozygous female x Hemizygous male from within the colony
    • Heterozygous female x Hemizygous male from within the colony
    Overall Breeding Performance
    Excellent
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes No Gene is X-linked
    Homozygotes are fertile: Yes No
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: 10 months 10 months
    Average litter size
    5-9
    Recommended wean age
    3-4 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    036899-UNC-SPERM Cryo-preserved spermatozoa $548.00 / Non-Profit Aliquot Approximate quantity2
    036899-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.