Strain Name:
B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax
Stock Number:
034830-JAX
Citation ID:
RRID:MMRRC_034830-JAX
Other Names:

This strain was formerly available as JAX Mice Stock 4807 (B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax  3xTg-AD)

Gene Information

Allele Symbol: Psen1tm1Mpm (Mus musculus (mouse))
Name: presenilin 1; targeted mutation 1, Mark P Mattson
Synonyms: PS1KI, PS1M146V, PS1M146VKI<->, PS-1 M146V KI
Chromosome: 12
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:1930937
Gene Symbol: Psen1 (Mus musculus (mouse))
Name: presenilin 1
Synonyms: S182, presenilin-1, PS-1, Ad3h, PS1
Chromosome: 12
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:1202717
Transgene: Tg(APPSwe,tauP301L)1Lfa (Mus musculus (mouse))
Name: transgene insertion 1, Frank M LaFerla; transgene insertion 1, Frank M LaFerla
Chromosome: unknown
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:2672831
ES Cell Line
N/A
Phenotype

Homozygous: Mice homozygous for all three mutant alleles (3xTg-AD; homozygous for the Psen1 mutation and homozygous for the co-injected APPSwe and tauP301L transgenes (Tg(APPSwe,tauP301L)1Lfa)) are viable, fertile and display no initial gross physical or behavioral abnormalities. Translation of the overexpressed transgenes appears to be restricted to the central nervous system, notably in Alzheimer's disease-relevant areas including the hippocampus and cerebral cortex. A progressive increase in amyloid beta peptide deposition is observed, with intracellular immunoreactivity being detected in some brain regions as early as 3-4 months. Synaptic transmission and long-term potentiation are demonstrably impaired in mice 6 months of age. Between 12-15 months aggregates of conformationally altered and hyperphosphorylated tau are detected in the hippocampus. This mutant mouse exhibits plaque and tangle pathology associated with synaptic dysfunction, traits similar to those observed in Alzheimer's disease patients.

Hemizygous: Hemizygous transgenic phenotype is expected to be similar to homozygote phenotype.

Heterozygous: Heterozygote knockout phenotype is expected to be similar to the wild-type phenotype. 

The donating investigator recently communicated (February 2014) that, in contrast to the initial observations, male transgenic mice may not exhibit the phenotypic traits originally described. No reports of diminished traits in female carriers have been reported.

Mammalian Phenotype Terms
Allelic Composition: Psen1tm1Mpm/Psen1tm1Mpm (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 )

Allelic Composition: Psen1tm1Mpm/Psen1tm1Shn (Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ )

Allelic Composition: Psen1tm1Mpm/Psen1tm1Pcw (Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Alzheimer Disease/drug therapy
  • Alzheimer Disease/genetics
  • Animals
  • Attention/drug effects
  • Attention/physiology
  • Conditioning, Operant/drug effects
  • Conditioning, Operant/physiology
  • Disease Models, Animal
  • Humans
  • Indans/administration & dosage
  • Indans/therapeutic use
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Piperidines/administration & dosage
  • Piperidines/therapeutic use
Strain Development
Single-cell embryos from mice bearing the presenilin PS1M146V knockin mutation on a mixed C7BL/6;129X1/SvJ;129S1/Sv genetic background (B6;129-Psen1tm1Mpm) were co-injected with two independent mutant human transgenes; amyloid beta precursor protein (APPSwe) and microtubule-associated protein tau (tauP30IL). Both transgenes integrated at the same locus and are under the control of the mouse Thy1.2 regulatory element. Founder mice (line B1) were mated to B6;129-Psen1tm1Mpm mice. Offspring from this cross were bred together, resulting in mice homozygous for all three alleles (3xTg-AD; homozygous for the Psen1 mutation and homozygous for the co-injected APPSwe and tauP301L transgenes (Tg(APPSwe,tauP301L)1Lfa)). Both male and female 3xTg-AD mice on the mixed C7BL/6;129X1/SvJ;129S1/Sv genetic background were sent to The Repository and bred together to establish this colony.
Suggested Control Mice
B6129SF2/J is an approximate control
  • Models for Human Disease
  • Neurobiology
Donor
Frank LaFerla, Ph.D., University of California, Irvine
Primary Reference
Romberg C, Mattson MP, Mughal MR, Bussey TJ, Saksida LM. Impaired attention in the 3xTgAD mouse model of Alzheimer's disease: rescue by donepezil (Aricept). J Neurosci. 2011 Mar 2;31(9):3500-7. (Medline PMID: 21368062)

Colony and Husbandry Informationon

When maintaining a live colony, mice that are homozygous for the Psen1 mutation and homozygous for the co-injected APPSwe and tauP301L transgenes (Tg(APPSwe,tauP301L)1Lfa) may be bred together.

Colony's Current Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
034830-JAX-MIX1-F
034830-JAX- MIX1-M
Homozygous Knockout / Homozygous Transgene female
Homozygous Knockout / Homozygous Transgene male
$185.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.